دورية أكاديمية
Isolation, toxinotyping and antimicrobial susceptibility testing of Clostridium perfringens isolated from Pakistan poultry.
| العنوان: | Isolation, toxinotyping and antimicrobial susceptibility testing of Clostridium perfringens isolated from Pakistan poultry. |
|---|---|
| المؤلفون: | Haider Z; Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: zulquernainh@gmail.com., Ali T; Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: tanveer.alituri93@gmail.com., Ullah A; Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: asimsamdani4@gmail.com., Basit A; Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: basitbch@gmail.com., Tahir H; Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: hamzatahir1974@gmail.com., Tariq H; Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: hafsa11994@gmail.com., Ilyas SZ; Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: zainabilyas@ymail.com., Hayat Z; Department of Animal Sciences, College of Agriculture, University of Sarghoda, Sarghoda, Pakistan; Department of Animal Sciences, CVAS- Jhang Campus, University of Veterinary & Animal Sciences, Lahore, Pakistan. Electronic address: zafar.hayat@uos.edu.pk., Rehman SU; Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. Electronic address: shafiq.mmg@pu.edu.pk. |
| المصدر: | Anaerobe [Anaerobe] 2022 Feb; Vol. 73, pp. 102499. Date of Electronic Publication: 2021 Dec 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 9505216 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8274 (Electronic) Linking ISSN: 10759964 NLM ISO Abbreviation: Anaerobe Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Academic Press Original Publication: London ; San Diego : Academic Press, c1995- |
| مواضيع طبية MeSH: | Bacterial Toxins*/analysis , Bacterial Toxins*/genetics , Clostridium Infections*/epidemiology , Clostridium Infections*/veterinary , Enteritis*/veterinary , Poultry Diseases*/epidemiology, Animals ; Anti-Bacterial Agents/pharmacology ; Chickens ; Clostridium perfringens/genetics ; Enterotoxins/genetics ; Pakistan ; Poultry |
| مستخلص: | Background and Objectives: Clostridium perfringens is a causative agent of enteric infections in animals including poultry by producing twenty different types of toxins. A single strain produces only a subset of these toxins, which form the basis of its classification into seven toxinotypes (A-G). C. perfringens toxinotype A is a widespread cause of necrotic enteritis (NE) in poultry. The current study was conducted to determine the prevalence of different toxins and antimicrobial susceptibility of C. perfringens isolated from Pakistan NE affected poultry. Methods: A total of 134 intestinal samples of the diseased birds were collected postmortem and processed for isolation of C. perfringens using tryptose sulphite cycloserine (TSC) agar supplemented with d-cycloserine. Isolates were confirmed by Gram's staining, biochemical and molecular analyses. Toxinotyping was performed by multiplex PCR. Antimicrobial susceptibility profile of isolates was performed by Kirby Bauer disc diffusion method. Results: A total of 34 strains of C. perfringens were isolated from 134 samples with prevalence rate of 25.37%. All the isolated strains were toxinotype A, as they were positive for alpha toxin (CPA) and negative for other tested toxins such as beta (CPB), epsilon (ETX), iota (ITX), enterotoxin (CPE), toxin perfringens large (TpeL) and necrotic B-like toxin (NetB). Interestingly, all the isolated strains of C. perfringens were multidrug resistant. The highest resistance was observed against Neomycin, Trimethoprim, Tetracycline and Lincomycin which are routinely used at Pakistan poultry production. Conclusion: C. perfringens toxinotype A is prevalent in Pakistan poultry. Incidence of C. perfringens with prevalence rate of 25.37% can pose serious threat to Pakistan's poultry industry given that all the isolated strains were multidrug resistant. Our findings highlight the need for new antibiotics and antibiotic alternatives to overcome multidrug resistance. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2021 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Alpha toxin; Antimicrobial susceptibility; Clostridium perfringens; Multilocus sequence typing; Multiplex PCR; Necrotic enteritis (NE); Toxinotyping |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Bacterial Toxins) 0 (Enterotoxins) |
| تواريخ الأحداث: | Date Created: 20211210 Date Completed: 20220316 Latest Revision: 20220316 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.anaerobe.2021.102499 |
| PMID: | 34890812 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-8274 |
|---|---|
| DOI: | 10.1016/j.anaerobe.2021.102499 |