دورية أكاديمية
Identification of mitochondrial RNA polymerase as a potential therapeutic target of osteosarcoma.
العنوان: | Identification of mitochondrial RNA polymerase as a potential therapeutic target of osteosarcoma. |
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المؤلفون: | Han QC; Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China., Zhang XY; Department of Orthopaedics, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China., Yan PH; Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China., Chen SF; Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China., Liu FF; Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China., Zhu YR; Department of Orthopedics, Affiliated Jiangyin Hospital of Medical College of Southeast University, Jiangyin, China. zyr13579@hotmail.com., Tian Q; Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China. jaytian2010@126.com. |
المصدر: | Cell death discovery [Cell Death Discov] 2021 Dec 14; Vol. 7 (1), pp. 393. Date of Electronic Publication: 2021 Dec 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 101665035 Publication Model: Electronic Cited Medium: Print ISSN: 2058-7716 (Print) Linking ISSN: 20587716 NLM ISO Abbreviation: Cell Death Discov Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [New York, NY] : Nature Publishing Group, [2015]- |
مستخلص: | POLRMT (RNA polymerase mitochondrial) is essential for transcription of mitochondrial genome encoding components of oxidative phosphorylation process. The current study tested POLRMT expression and its potential function in osteosarcoma (OS). The Cancer Genome Atlas (TCGA) cohorts and Gene Expression Profiling Interactive Analysis (GEPIA) database both show that POLRMT transcripts are elevated in OS tissues. In addition, POLRMT mRNA and protein levels were upregulated in local OS tissues as well as in established and primary human OS cells. In different OS cells, shRNA-induced stable knockdown of POLRMT decreased cell viability, proliferation, migration, and invasion, whiling inducing apoptosis activation. CRISPR/Cas9-induced POLRMT knockout induced potent anti-OS cell activity as well. Conversely, in primary OS cells ectopic POLRMT overexpression accelerated cell proliferation and migration. In vivo, intratumoral injection of adeno-associated virus-packed POLRMT shRNA potently inhibited U2OS xenograft growth in nude mice. Importantly, levels of mitochondrial DNA, mitochondrial transcripts and expression of respiratory chain complex subunits were significantly decreased in U2OS xenografts with POLRMT shRNA virus injection. Together, POLRMT is overexpressed in human OS, promoting cell growth in vitro and in vivo. POLRMT could be a novel therapeutic target for OS. (© 2021. The Author(s).) |
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معلومات مُعتمدة: | 82002850 National Natural Science Foundation of China (National Science Foundation of China) |
تواريخ الأحداث: | Date Created: 20211215 Latest Revision: 20230210 |
رمز التحديث: | 20230211 |
مُعرف محوري في PubMed: | PMC8671410 |
DOI: | 10.1038/s41420-021-00780-x |
PMID: | 34907167 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2058-7716 |
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DOI: | 10.1038/s41420-021-00780-x |