دورية أكاديمية
Comparison of novel rapid diagnostic of blood culture identification and antimicrobial susceptibility testing by Accelerate Pheno system and BioFire FilmArray Blood Culture Identification and BioFire FilmArray Blood Culture Identification 2 panels.
العنوان: | Comparison of novel rapid diagnostic of blood culture identification and antimicrobial susceptibility testing by Accelerate Pheno system and BioFire FilmArray Blood Culture Identification and BioFire FilmArray Blood Culture Identification 2 panels. |
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المؤلفون: | Sze DTT; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China., Lau CCY; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China., Chan TM; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China., Ma ESK; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China., Tang BSF; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China. bonetang@hksh.com.; Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong, Special Administrative Region, China. bonetang@hksh.com. |
المصدر: | BMC microbiology [BMC Microbiol] 2021 Dec 18; Vol. 21 (1), pp. 350. Date of Electronic Publication: 2021 Dec 18. |
نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : BioMed Central, [2001- |
مواضيع طبية MeSH: | Blood Culture/*methods , Microbial Sensitivity Tests/*methods, Anti-Bacterial Agents/pharmacology ; Bacteria/classification ; Bacteria/drug effects ; Bacteria/isolation & purification ; Blood Culture/standards ; Drug Resistance, Microbial/drug effects ; Drug Resistance, Microbial/genetics ; Fungi/classification ; Fungi/drug effects ; Fungi/isolation & purification ; Genotype ; Humans ; Microbial Sensitivity Tests/standards ; Phenotype ; Sensitivity and Specificity ; Sepsis/diagnosis ; Sepsis/microbiology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Time Factors ; Workflow |
مستخلص: | Background: Conventional turnaround time (TAT) for positive blood culture (PBC) identification (ID) and antimicrobial susceptibility testing (AST) is 2-3 days. We evaluated the TAT and ID/AST performance using clinical and seeded samples directly from PBC bottles with different commercial approaches: (1) Accelerate Pheno® system (Pheno) for ID/AST; (2) BioFire® FilmArray® Blood Culture Identification (BCID) Panel and/ or BCID2 for ID; (3) direct AST by VITEK® 2 (direct AST); and (4) overnight culture using VITEK® 2 colony AST. Results: A total of 141 PBC samples were included in this evaluation. Using MALDI-TOF (Bruker MALDI Biotyper) as the reference method for ID, the overall monomicrobial ID sensitivity/specificity are as follows: Pheno 97.9/99.9%; BCID 100/100%; and BCID2 100/100%, respectively. For AST performance, broth microdilution (BMD) was used as the reference method. For gram-negatives, overall categorical and essential agreements (CA/EA) for each method were: Pheno 90.3/93.2%; direct AST 92.6/88.5%; colony AST 94.4/89.5%, respectively. For gram-positives, the overall CA/EAs were as follows: Pheno 97.2/98.89%; direct AST 97.2/100%; colony AST 97.2/100%, respectively. The BCID/BCID2 and direct AST TATs were around 9-20 h (1/9-19 h for ID with resistance markers/AST), with 15 min/sample hands-on time. In comparison, Pheno TATs were around 8-10 h (1.5/7 h for ID/AST) with 2 min/sample hands-on time, maintains a clinically relevant fast report of antibiotic minimal inhibitory concentration (MIC) and allows for less TAT and hands-on time. Conclusion: In conclusion, to the best of our knowledge, this is the first study conducted as such in Asia; all studied approaches achieved satisfactory performance, factors such as TAT, panel of antibiotics choices and hands-on time should be considered for the selection of appropriate rapid ID and AST of PBC methods in different laboratory settings. (© 2021. The Author(s).) |
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فهرسة مساهمة: | Keywords: Antimicrobial resistance; Blood culture; Bloodstream infections; Filmarray; Identification; Pheno; Rapid tests; Susceptibility testing |
المشرفين على المادة: | 0 (Anti-Bacterial Agents) |
تواريخ الأحداث: | Date Created: 20211219 Date Completed: 20220127 Latest Revision: 20220127 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8684256 |
DOI: | 10.1186/s12866-021-02403-y |
PMID: | 34922463 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1471-2180 |
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DOI: | 10.1186/s12866-021-02403-y |