دورية أكاديمية
Assessment of the utility of measuring Thyroglobulin mRNA levels by Quantitative Real-Time PCR in the follow-up of differentiated thyroid cancer patients.
| العنوان: | Assessment of the utility of measuring Thyroglobulin mRNA levels by Quantitative Real-Time PCR in the follow-up of differentiated thyroid cancer patients. |
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| المؤلفون: | Russo-Picasso MF; Servicio de Endocrinología, Metabolismo y Medicina Nuclear, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: maria.russopicasso@hospitalitaliano.org.ar., Serra MP; Laboratorio de Biología Molecular, Servicio de Endocrinología, Metabolismo y Medicina Nuclear, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina., Viale ML; Laboratorio de Biología Molecular, Servicio de Endocrinología, Metabolismo y Medicina Nuclear, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina., Puga MC; Sección Investigación no esponsoreada, Departamento de Investigación, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina., Terrasa S; Sección Investigación no esponsoreada, Departamento de Investigación, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina., Kozak AE; Laboratorio Hormonal, Servicio de Endocrinología, Metabolismo y Medicina Nuclear, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina., Califano I; Servicio de Endocrinología, Instituto de Oncología Ángel H. Roffo, Ciudad Autónoma de Buenos Aires, Argentina., Boccalatte LA; Sección de Cabeza y Cuello, Servicio de Cirugía General, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina., Stigliano A; Laboratorio de Biología Molecular, Servicio de Endocrinología, Metabolismo y Medicina Nuclear, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina., Fainstein-Day P; Servicio de Endocrinología, Metabolismo y Medicina Nuclear, Hospital Italiano de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina. |
| المصدر: | Endocrinologia, diabetes y nutricion [Endocrinol Diabetes Nutr (Engl Ed)] 2021 Dec; Vol. 68 (10), pp. 680-688. Date of Electronic Publication: 2021 Dec 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Country of Publication: Spain NLM ID: 101717565 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2530-0180 (Electronic) Linking ISSN: 25300180 NLM ISO Abbreviation: Endocrinol Diabetes Nutr (Engl Ed) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Barcelona] : [Elsevier España], 2017- |
| مواضيع طبية MeSH: | Adenocarcinoma, Follicular* , Thyroid Neoplasms*/diagnosis , Thyroid Neoplasms*/genetics, Follow-Up Studies ; Humans ; Prospective Studies ; RNA, Messenger/genetics ; Real-Time Polymerase Chain Reaction ; Thyroglobulin/genetics |
| مستخلص: | Introduction: The determination of thyroglobulin levels by immunoassay and imaging studies is subject to interference by antithyroglobulin antibodies in up to 30% of cases, suggesting a need to find alternative methods for the follow-up of a significant number of thyroid cancer patients. Objectives: Assess the sensitivity, specificity, and predictive values of thyroglobulin messenger RNA levels measured by quantitative Real Time-PCR (qRT-PCR) in the blood of patients followed for differentiated thyroid cancer. Methods: This is a prospective study of Tg-mRNA levels measured with qRT-PCR. A peripheral blood sample was taken in patients with excellent response (69) and with structural incomplete response to treatment (23). Results were analysed using the Unity Real-Time program and expressed as fg/μg RNA. A Receiver Operating Characteristic curve was constructed to assess Tg-mRNA cut-off values. Results: Tg-mRNA levels were not significantly different between the group with excellent response [0.10 fg/μg RNA (0.08-0.17)] and the group with incomplete structural response [0.133 fg/μg RNA (0.07-0.33)] (P < .06). Test sensitivity was 69.6%, specificity was 59.4%, negative predictive value was 85.4% and positive predictive value 36.4% CONCLUSIONS: Our experience shows that this technique could be useful as a rule-out test in selected cases, but its low sensitivity and specificity preclude its usefulness as a first-line test. (Copyright © 2021 SEEN and SED. Published by Elsevier España, S.L.U. All rights reserved.) |
| فهرسة مساهمة: | Keywords: ARN mensajero de tiroglobulina; Cancer follow-up; Cáncer de tiroides; Cáncer diferenciado de tiroides; Differentiated thyroid cancer; RT-PCR; Seguimiento cáncer; Thyroglobulin; Thyroglobulin messenger RNA; Thyroid cancer; Tiroglobulina |
| المشرفين على المادة: | 0 (RNA, Messenger) 9010-34-8 (Thyroglobulin) |
| تواريخ الأحداث: | Date Created: 20211220 Date Completed: 20220117 Latest Revision: 20220117 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.endien.2021.12.001 |
| PMID: | 34924156 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2530-0180 |
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| DOI: | 10.1016/j.endien.2021.12.001 |