دورية أكاديمية
أعرض في EDS

USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3/COL6A5 stabilisation.

التفاصيل البيبلوغرافية
العنوان: USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3/COL6A5 stabilisation.
المؤلفون: Wu X; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Wang H; Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.; Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Guangzhou, 510515, China., Zhu D; Department of Medical Examination, Rocket Army Guangzhou Special Service Convalescent Center, Guangzhou, 510515, China., Chai Y; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Wang J; Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China., Dai W; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Xiao Y; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Tang W; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Li J; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Hong L; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Pei M; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Zhang J; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Lin Z; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China., Wang J; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. jidewang55@163.com.; Department of Gastroenterology, Longgang District People's Hospital, Shenzhen, 518172, China. jidewang55@163.com., Li A; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. lam0725@163.com.; Department of Gastroenterology, Longgang District People's Hospital, Shenzhen, 518172, China. lam0725@163.com., Liu S; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. liuside2011@163.com.; Department of Gastroenterology, Longgang District People's Hospital, Shenzhen, 518172, China. liuside2011@163.com.
المصدر: Cell death & disease [Cell Death Dis] 2021 Dec 20; Vol. 13 (1), pp. 10. Date of Electronic Publication: 2021 Dec 20.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101524092 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-4889 (Electronic) NLM ISO Abbreviation: Cell Death Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Pub. Group
مواضيع طبية MeSH: Carcinogenesis/*metabolism , Collagen Type IX/*metabolism , Collagen Type VI/*metabolism , Lung Neoplasms/*secondary , Signal Transduction/*genetics , Stomach Neoplasms/*metabolism , Stomach Neoplasms/*pathology , Ubiquitin-Specific Proteases/*metabolism , Ubiquitination/*genetics, Animals ; Carcinogenesis/genetics ; Cell Line, Tumor ; Collagen Type IX/genetics ; Collagen Type VI/genetics ; Disease Models, Animal ; Heterografts ; Humans ; Lung Neoplasms/metabolism ; Mice ; Mice, Nude ; Neoplasm Transplantation/methods ; RNA Interference ; Transfection/methods ; Ubiquitin-Specific Proteases/genetics
مستخلص: As an important regulator of intracellular protein degradation, the mechanism of the deubiquitinating enzyme family in tumour metastasis has received increasing attention. Our previous study revealed that USP3 promotes tumour progression and is highly expressed in gastric cancer (GC). Herein, we report two critical targets, COL9A3 and COL6A5, downstream of USP3, via the isobaric tags for relative and absolute quantification technique. Mechanistically, we observed that USP3 interacted with and stabilised COL9A3 and COL6A5 via deubiquitination in GC. Importantly, we found that COL9A3 and COL6A5 were essential mediators of USP3-modulated oncogenic activity in vitro and in vivo. Examination of clinical samples confirmed that elevated expression of USP3, concomitant with increased COL9A3 and COL6A5 abundance, correlates with human GC progression. These data suggest that USP3 promotes GC progression and metastasis by deubiquitinating COL9A3 and COL6A5. These findings identify a mechanism of GC metastasis regarding USP3-mediated deubiquitinating enzyme activity and suggest potential therapeutic targets for GC management.
(© 2021. The Author(s).)
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المشرفين على المادة: 0 (COL6A5 protein, human)
0 (COL9A3 protein, human)
0 (Collagen Type IX)
0 (Collagen Type VI)
EC 3.4.19.12 (USP3 protein, human)
EC 3.4.19.12 (Ubiquitin-Specific Proteases)
تواريخ الأحداث: Date Created: 20211221 Date Completed: 20220325 Latest Revision: 20231108
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8688524
DOI: 10.1038/s41419-021-04460-7
PMID: 34930901
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-4889
DOI:10.1038/s41419-021-04460-7