دورية أكاديمية

Nanoribbon Biosensor in the Detection of miRNAs Associated with Colorectal Cancer.

التفاصيل البيبلوغرافية
العنوان: Nanoribbon Biosensor in the Detection of miRNAs Associated with Colorectal Cancer.
المؤلفون: Ivanov YD; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia., Goldaeva KV; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia., Malsagova KA; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia., Pleshakova TO; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia., Galiullin RA; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia., Popov VP; Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia., Kushlinskii NE; N.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia., Alferov AA; N.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia., Enikeev DV; Institute of Urology and Reproductive Health, Sechenov University, 119992 Moscow, Russia., Potoldykova NV; Institute of Urology and Reproductive Health, Sechenov University, 119992 Moscow, Russia., Archakov AI; Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia.
المصدر: Micromachines [Micromachines (Basel)] 2021 Dec 18; Vol. 12 (12). Date of Electronic Publication: 2021 Dec 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101640903 Publication Model: Electronic Cited Medium: Print ISSN: 2072-666X (Print) Linking ISSN: 2072666X NLM ISO Abbreviation: Micromachines (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2010]-
مستخلص: A nanoribbon biosensor (NRBS) was developed to register synthetic DNAs that simulate and are analogous to miRNA-17-3p associated with colorectal cancer. Using this nanoribbon biosensor, the ability to detect miRNA-17-3p in the blood plasma of a patient diagnosed with colorectal cancer has been demonstrated. The sensing element of the NRBS was a nanochip based on a silicon-on-insulator (SOI) nanostructure. The nanochip included an array of 10 nanoribbons and was designed with the implementation of top-down technology. For biospecific recognition of miRNA-17-3p, the nanochip was modified with DNA probes specific for miRNA-17-3p. The performance of the nanochip was preliminarily tested on model DNA oligonucleotides, which are synthetic analogues of miRNA-17-3p, and a detection limit of ~10 -17 M was achieved. The results of this work can be used in the development of serological diagnostic systems for early detection of colorectal cancer.
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معلومات مُعتمدة: Agreement No. 075-15-2021-993, unique project ID: RF00121X0004 The Ministry of Education and Science of the Russian Federation
فهرسة مساهمة: Keywords: cancer; colorectal cancer; miRNA; nanoribbon; nanoribbon biosensor; silicon-on-insulator
تواريخ الأحداث: Date Created: 20211224 Latest Revision: 20240404
رمز التحديث: 20240404
مُعرف محوري في PubMed: PMC8705149
DOI: 10.3390/mi12121581
PMID: 34945431
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-666X
DOI:10.3390/mi12121581