دورية أكاديمية
أعرض في EDS

Comparative analysis between cytomorphology and flow cytometry methods in central nervous system infiltration assessment in oncohematological patients.

التفاصيل البيبلوغرافية
العنوان: Comparative analysis between cytomorphology and flow cytometry methods in central nervous system infiltration assessment in oncohematological patients.
المؤلفون: Rocha JMC; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. Electronic address: camargos.juliana@gmail.com., Murao M; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Cancela CSP; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., de Oliveira LP; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Perim LP; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Santos JP; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., de Oliveira BM; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
المصدر: Hematology, transfusion and cell therapy [Hematol Transfus Cell Ther] 2023 Apr-Jun; Vol. 45 (2), pp. 188-195. Date of Electronic Publication: 2021 Dec 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Editora Ltda Country of Publication: Brazil NLM ID: 101725732 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2531-1387 (Electronic) Linking ISSN: 25311379 NLM ISO Abbreviation: Hematol Transfus Cell Ther Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Rio de Janeiro, Brazil] : Elsevier Editora Ltda, [2018]-
مستخلص: Introduction: Oncohematological patients require the evaluation for possible infiltration of the central nervous system (CNS) by neoplastic cells at diagnosis and/or during the monitoring of the chemotherapeutic treatment. Morphological analysis using conventional microscopy is considered the method of choice to evaluate the cerebrospinal fluid (CSF) samples, despite technical limitations.
Objective: This study aimed to compare the performance of the cytomorphology and flow cytometric immunophenotyping (FC) in the detection of CNS infiltration.
Method: We evaluated 520 CSF samples collected from 287 oncohematological patients for whom the detection of neoplastic cells was simultaneously requested by cytomorphology and FC.
Results: Laboratory analyses revealed 435/520 (83.7%) conclusive results by the two methods evaluated, among which 385 (88.5%) were concordant. Discordance between the methods was observed in 50/435 (11.5%) samples, 45 (90%) being positive by FC. Furthermore, the FC defined the results in 69/72 (95.8%) inconclusive samples by cytomorphology. The positivity of FC was particularly higher among hypocellular samples. Among 431 samples with a cell count of < 5/μL, the FC identified neoplastic cells in 75 (17.4%), while the cytomorphology reported positive results in 26 (6%). Among the samples that presented adequate cell recovery for evaluation by both methods (506/520), the comparative analysis between FC and cytomorphology revealed a Kappa coefficient of 0.45 (CI: 0.37-0.52), interpreted as a moderate agreement.
Conclusion: The data showed that the CSF analysis by FC helps in the definition of CNS infiltration by neoplastic cells, particularly in the cases with dubious morphological analysis or in the evaluation of samples with low cellularity.
Competing Interests: Conflicts of interest The authors certify that they have no financial interest in the subject matter or materials discussed in this manuscript.
(Copyright © 2021 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier España, S.L.U. All rights reserved.)
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فهرسة مساهمة: Keywords: Cell morphology; Central nervous system; Cerebrospinal fluid; Flow cytometry; Hematological neoplasms
تواريخ الأحداث: Date Created: 20211224 Latest Revision: 20230610
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10244250
DOI: 10.1016/j.htct.2021.09.016
PMID: 34949559
قاعدة البيانات: MEDLINE
الوصف
تدمد:2531-1387
DOI:10.1016/j.htct.2021.09.016