دورية أكاديمية
Membrane Remodeling and Matrix Dispersal Intermediates During Mammalian Acrosomal Exocytosis.
العنوان: | Membrane Remodeling and Matrix Dispersal Intermediates During Mammalian Acrosomal Exocytosis. |
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المؤلفون: | Leung MR; Bijvoet Centre for Biomolecular Research, Utrecht University, Utrecht, Netherlands.; The Division of Structural Biology, Wellcome Centre for Human Genetics, The University of Oxford, Oxford, United Kingdom., Ravi RT; Bijvoet Centre for Biomolecular Research, Utrecht University, Utrecht, Netherlands., Gadella BM; Department of Farm and Animal Health and Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., Zeev-Ben-Mordehai T; Bijvoet Centre for Biomolecular Research, Utrecht University, Utrecht, Netherlands.; The Division of Structural Biology, Wellcome Centre for Human Genetics, The University of Oxford, Oxford, United Kingdom. |
المصدر: | Frontiers in cell and developmental biology [Front Cell Dev Biol] 2021 Dec 10; Vol. 9, pp. 765673. Date of Electronic Publication: 2021 Dec 10 (Print Publication: 2021). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101630250 Publication Model: eCollection Cited Medium: Print ISSN: 2296-634X (Print) Linking ISSN: 2296634X NLM ISO Abbreviation: Front Cell Dev Biol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2013]- |
مستخلص: | To become fertilization-competent, mammalian sperm must undergo a complex series of biochemical and morphological changes in the female reproductive tract. These changes, collectively called capacitation, culminate in the exocytosis of the acrosome, a large vesicle overlying the nucleus. Acrosomal exocytosis is not an all-or-nothing event but rather a regulated process in which vesicle cargo disperses gradually. However, the structural mechanisms underlying this controlled release remain undefined. In addition, unlike other exocytotic events, fusing membranes are shed as vesicles; the cell thus loses the entire anterior two-thirds of its plasma membrane and yet remains intact, while the remaining nonvesiculated plasma membrane becomes fusogenic. Precisely how cell integrity is maintained throughout this drastic vesiculation process is unclear, as is how it ultimately leads to the acquisition of fusion competence. Here, we use cryoelectron tomography to visualize these processes in unfixed, unstained, fully hydrated sperm. We show that paracrystalline structures within the acrosome disassemble during capacitation and acrosomal exocytosis, representing a plausible mechanism for gradual dispersal of the acrosomal matrix. We find that the architecture of the sperm head supports an atypical membrane fission-fusion pathway that maintains cell integrity. Finally, we detail how the acrosome reaction transforms both the micron-scale topography and the nanoscale protein landscape of the sperm surface, thus priming the sperm for fertilization. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Leung, Ravi, Gadella and Zeev-Ben-Mordehai.) |
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فهرسة مساهمة: | Keywords: acrosome reaction; cryo-elecron tomography; fertilization; mammalian sperm; membrane fusion |
تواريخ الأحداث: | Date Created: 20211227 Latest Revision: 20211228 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC8708559 |
DOI: | 10.3389/fcell.2021.765673 |
PMID: | 34957098 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2296-634X |
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DOI: | 10.3389/fcell.2021.765673 |