دورية أكاديمية
أعرض في EDS

A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders.

التفاصيل البيبلوغرافية
العنوان: A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders.
المؤلفون: Lopes JIF; Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio, Rua Frei Caneca, 8, Centro, Rio de Janeiro CEP 20211-030, Brazil.; Laboratório de Desenvolvimento Tecnológico em Virologia, Oswaldo Cruz Institute/Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro CEP 21040-900, Brazil., da Costa Silva CA; Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio, Rua Frei Caneca, 8, Centro, Rio de Janeiro CEP 20211-030, Brazil., Cunha RG; Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio, Rua Frei Caneca, 8, Centro, Rio de Janeiro CEP 20211-030, Brazil., Soares AM; Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio, Rua Frei Caneca, 8, Centro, Rio de Janeiro CEP 20211-030, Brazil., Lopes MED; Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio, Rua Frei Caneca, 8, Centro, Rio de Janeiro CEP 20211-030, Brazil., da Conceição Neto OC; Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio, Rua Frei Caneca, 8, Centro, Rio de Janeiro CEP 20211-030, Brazil., Alves ADR; Laboratório de Desenvolvimento Tecnológico em Virologia, Oswaldo Cruz Institute/Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro CEP 21040-900, Brazil., da Costa Nunes Pimentel Coelho WL; Laboratório de Desenvolvimento Tecnológico em Virologia, Oswaldo Cruz Institute/Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro CEP 21040-900, Brazil., Amorim Filho L; Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio, Rua Frei Caneca, 8, Centro, Rio de Janeiro CEP 20211-030, Brazil., Amado Leon LA; Laboratório de Desenvolvimento Tecnológico em Virologia, Oswaldo Cruz Institute/Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro CEP 21040-900, Brazil.
المصدر: Viruses [Viruses] 2021 Nov 25; Vol. 13 (12). Date of Electronic Publication: 2021 Nov 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Blood Coagulation Disorders/*complications , COVID-19/*complications , COVID-19/*diagnosis , Hemorrhagic Disorders/*complications , SARS-CoV-2/*isolation & purification, Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19/virology ; COVID-19 Testing ; Child ; Child, Preschool ; Cohort Studies ; Diagnostic Tests, Routine ; Female ; Health Personnel ; Humans ; Infant ; Male ; Middle Aged ; Saliva ; Viral Load ; Young Adult
مستخلص: Diagnosis of SARS-CoV-2 infections is mostly based on the nasopharyngeal swabs (NPS). However, this collection is invasive and uncomfortable, especially for children and patients with coagulopathies, whose NPS collection often causes bleeding. Thus, the aim of this study was to evaluate the usefulness and accuracy of saliva for the diagnosis of COVID-19 in patients presenting bleeding disorders. Samples of NPS, oropharyngeal swabs (OPS), and saliva were collected simultaneously from 1159 hospitalized patients with hematological diseases and from 524 healthcare workers, both symptomatic and asymptomatic for SARS-CoV-2. All samples were evaluated for SARS-CoV-2 by qRT-PCR. SARS-CoV-2 was detected in NPS, OPS and saliva from 16.9%, 14.4% and 15.6% individuals, respectively. Tests in saliva showed sensitivity, specificity, and overall agreement of 73.3%, 96.9% and 92.7% (=0.74), respectively. Salivary tests had good accuracy (AUC = 0.7) for discriminating negative and positive qRT-PCR for SARS-CoV-2. Higher sensitivity was observed in symptomatic than in non-symptomatic patients, as well as in healthy subjects than in patients with hematological disease, in both OPS and saliva. The mean viral load in NPS was significantly higher than in OPS and in saliva samples ( p < 0.001). Saliva is a good diagnostic tool to detect SARS-CoV-2, especially among patients symptomatic for COVID-19, and is a valuable specimen for mass screening of hospitalized patients with hematological diseases, especially for those that with bleeding disorders.
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فهرسة مساهمة: Keywords: COVID-19; SARS-CoV-2; bleeding disorders; diagnosis; hematological disease; saliva
تواريخ الأحداث: Date Created: 20211228 Date Completed: 20220118 Latest Revision: 20220118
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8707508
DOI: 10.3390/v13122361
PMID: 34960630
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v13122361