دورية أكاديمية
أعرض في EDS

Characterization and Tissue Tropism of Newly Identified Iflavirus and Negeviruses in Glossina morsitans morsitans Tsetse Flies.

التفاصيل البيبلوغرافية
العنوان: Characterization and Tissue Tropism of Newly Identified Iflavirus and Negeviruses in Glossina morsitans morsitans Tsetse Flies.
المؤلفون: Meki IK; Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria., Huditz HI; Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria.; Laboratory of Virology, Wageningen University and Research, 6708 PB Wageningen, The Netherlands., Strunov A; Lab Genome Dynamics, Department Cell & Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstraße 17, 1090 Vienna, Austria., van der Vlugt RAA; Laboratory of Virology, Wageningen University and Research, 6708 PB Wageningen, The Netherlands., Kariithi HM; Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria.; Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, Agricultural Research Service, USDA-ARS, Athens, GA 30605, USA.; Biotechnology Research Center, Kenya Agricultural and Livestock Research Organization, Nairobi P.O. Box 57811-00200, Kenya., Rezapanah M; Iranian Research Institute of Plant Protection (IRIPP), Agricultural Research Education and Extension Organization (AREEO), Tehran 19395, Iran., Miller WJ; Lab Genome Dynamics, Department Cell & Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstraße 17, 1090 Vienna, Austria., Vlak JM; Laboratory of Virology, Wageningen University and Research, 6708 PB Wageningen, The Netherlands., van Oers MM; Laboratory of Virology, Wageningen University and Research, 6708 PB Wageningen, The Netherlands., Abd-Alla AMM; Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria.
المصدر: Viruses [Viruses] 2021 Dec 10; Vol. 13 (12). Date of Electronic Publication: 2021 Dec 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Viral Tropism*, Insect Viruses/*physiology , Positive-Strand RNA Viruses/*physiology , Tsetse Flies/*virology, Animals ; Brain/virology ; Digestive System/virology ; Fat Body/virology ; Female ; Genitalia/virology ; Genome, Viral ; Insect Viruses/classification ; Insect Viruses/genetics ; Insect Viruses/isolation & purification ; Male ; Phylogeny ; Positive-Strand RNA Viruses/classification ; Positive-Strand RNA Viruses/genetics ; Positive-Strand RNA Viruses/isolation & purification ; Salivary Glands/virology ; Virus Replication
مستخلص: Tsetse flies cause major health and economic problems as they transmit trypanosomes causing sleeping sickness in humans (Human African Trypanosomosis, HAT) and nagana in animals (African Animal Trypanosomosis, AAT). A solution to control the spread of these flies and their associated diseases is the implementation of the Sterile Insect Technique (SIT). For successful application of SIT, it is important to establish and maintain healthy insect colonies and produce flies with competitive fitness. However, mass production of tsetse is threatened by covert virus infections, such as the Glossina pallidipes salivary gland hypertrophy virus (GpSGHV). This virus infection can switch from a covert asymptomatic to an overt symptomatic state and cause the collapse of an entire fly colony. Although the effects of GpSGHV infections can be mitigated, the presence of other covert viruses threaten tsetse mass production. Here we demonstrated the presence of two single-stranded RNA viruses isolated from Glossina morsitans morsitans originating from a colony at the Seibersdorf rearing facility. The genome organization and the phylogenetic analysis based on the RNA-dependent RNA polymerase (RdRp) revealed that the two viruses belong to the genera Iflavirus and Negevirus , respectively. The names proposed for the two viruses are Glossina morsitans morsitans iflavirus (GmmIV) and Glossina morsitans morsitans negevirus (GmmNegeV). The GmmIV genome is 9685 nucleotides long with a poly(A) tail and encodes a single polyprotein processed into structural and non-structural viral proteins. The GmmNegeV genome consists of 8140 nucleotides and contains two major overlapping open reading frames (ORF1 and ORF2). ORF1 encodes the largest protein which includes a methyltransferase domain, a ribosomal RNA methyltransferase domain, a helicase domain and a RdRp domain. In this study, a selective RT-qPCR assay to detect the presence of the negative RNA strand for both GmmIV and GmmNegeV viruses proved that both viruses replicate in G. m. morsitans . We analyzed the tissue tropism of these viruses in G. m. morsitans by RNA-FISH to decipher their mode of transmission. Our results demonstrate that both viruses can be found not only in the host's brain and fat bodies but also in their reproductive organs, and in milk and salivary glands. These findings suggest a potential horizontal viral transmission during feeding and/or a vertically viral transmission from parent to offspring. Although the impact of GmmIV and GmmNegeV in tsetse rearing facilities is still unknown, none of the currently infected tsetse species show any signs of disease from these viruses.
References: Infect Genet Evol. 2009 Jan;9(1):124-41. (PMID: 18992846)
Sci Rep. 2017 Jul 5;7(1):4699. (PMID: 28680117)
Viruses. 2014 Nov 12;6(11):4346-57. (PMID: 25398046)
Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):12133-8. (PMID: 19587241)
Infect Genet Evol. 2015 Dec;36:184-189. (PMID: 26391183)
Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6814-9. (PMID: 10359795)
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15. (PMID: 12824337)
J Virol. 2013 Mar;87(5):2475-88. (PMID: 23255793)
Nucleic Acids Res. 2001 Dec 15;29(24):5079-89. (PMID: 11812840)
Proc Biol Sci. 2010 Aug 7;277(1692):2389-97. (PMID: 20356887)
Med Vet Entomol. 1993 Oct;7(4):377-83. (PMID: 8268495)
BMC Microbiol. 2018 Nov 23;18(Suppl 1):183. (PMID: 30470186)
Appl Environ Microbiol. 2008 Oct;74(19):5965-74. (PMID: 18689507)
Mutat Res. 2002 Jun;511(2):113-32. (PMID: 12052430)
Algorithms Mol Biol. 2011 Nov 24;6:26. (PMID: 22115189)
FEBS Lett. 2007 Jun 26;581(16):3120-6. (PMID: 17568583)
PLoS One. 2017 May 9;12(5):e0175157. (PMID: 28486521)
Mol Biol Evol. 2013 Dec;30(12):2725-9. (PMID: 24132122)
Virology. 2017 Apr;504:152-167. (PMID: 28193550)
J Gen Virol. 2006 Dec;87(Pt 12):3667-3677. (PMID: 17098984)
Front Microbiol. 2018 Apr 09;9:701. (PMID: 29686664)
Virus Res. 2010 Jun;150(1-2):103-10. (PMID: 20214934)
BMC Microbiol. 2018 Nov 23;18(Suppl 1):140. (PMID: 30470185)
J Gen Virol. 2006 Nov;87(Pt 11):3397-3407. (PMID: 17030876)
J Virol. 2008 May;82(9):4595-611. (PMID: 18272583)
Proc Natl Acad Sci U S A. 2020 Sep 29;117(39):24475-24483. (PMID: 32913052)
PLoS Negl Trop Dis. 2011 Aug;5(8):e1220. (PMID: 21912708)
Virology. 2015 Sep;483:318-28. (PMID: 26057025)
PeerJ. 2016 Mar 03;4:e1687. (PMID: 26966651)
Ital J Food Saf. 2015 Sep 25;4(3):5364. (PMID: 27800411)
Proc Natl Acad Sci U S A. 2018 Nov 13;115(46):11790-11795. (PMID: 30373823)
Nature. 2016 Dec 22;540(7634):539-543. (PMID: 27880757)
PLoS One. 2013 Apr 22;8(4):e61150. (PMID: 23613801)
فهرسة مساهمة: Keywords: FISH; Iflaviridae; RNA viruses; mass rearing; stellaris probes; sterile insect technique
تواريخ الأحداث: Date Created: 20211228 Date Completed: 20220131 Latest Revision: 20220131
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8704047
DOI: 10.3390/v13122472
PMID: 34960741
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v13122472