دورية أكاديمية
Colorimetric and Fluorometric Profiling of Advanced Glycation End Products.
| العنوان: | Colorimetric and Fluorometric Profiling of Advanced Glycation End Products. |
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| المؤلفون: | Ammanath G; Centre for Biomimetic Sensor Science, Nanyang Technological University, 637553 Singapore, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, 639798 Singapore, Singapore., Delachi CG; Centre for Biomimetic Sensor Science, Nanyang Technological University, 637553 Singapore, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, 639798 Singapore, Singapore., Karabacak S; Department of Chemistry, Izmir Institute of Technology, Urla, 35430 Izmir, Turkey., Ali Y; Lee Kong Chian School of Medicine, Nanyang Technological University, 308232 Singapore, Singapore., Boehm BO; Lee Kong Chian School of Medicine, Nanyang Technological University, 308232 Singapore, Singapore., Yildiz UH; Department of Chemistry, Izmir Institute of Technology, Urla, 35430 Izmir, Turkey., Alagappan P; Centre for Biomimetic Sensor Science, Nanyang Technological University, 637553 Singapore, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, 639798 Singapore, Singapore., Liedberg B; Centre for Biomimetic Sensor Science, Nanyang Technological University, 637553 Singapore, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, 639798 Singapore, Singapore. |
| المصدر: | ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2022 Jan 12; Vol. 14 (1), pp. 94-103. Date of Electronic Publication: 2021 Dec 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society |
| مواضيع طبية MeSH: | Colorimetry/*methods , Glycation End Products, Advanced/*blood , Spectrometry, Fluorescence/*methods, Aptamers, Nucleotide/chemistry ; Biomarkers/blood ; Biomarkers/chemistry ; Fluorescent Dyes/chemistry ; Glycation End Products, Advanced/chemistry ; Humans ; Limit of Detection ; Polymers/chemistry ; Thiophenes/chemistry |
| مستخلص: | Profiling of advanced glycation end products (AGEs) is an emerging area of clinical significance for disease diagnosis and prognosis. Typically, concentrations of AGEs are estimated in laboratories by trained personnel using sophisticated equipment. Herein, a facile approach for colorimetric and fluorometric profiling of AGEs is reported for rapid and on-site analysis. The concentrations of AGE levels in plasma are estimated via changes in optical properties of polythiophenes (PTs) upon interaction with aptamers (Apts) in the presence and in the absence of AGEs. To validate the proposed approach, glyceraldehyde-derived AGEs (AGE class 1 [AGE1]), the biomarker associated with cardiovascular diseases and diabetes, are used as a model system. Colorimetric analysis yielded linear responses for AGE1 for clinically relevant concentration ranges between 1.5 and 300 μg/mL with a limit of detection (LOD) of ∼1.3 μg/mL. Subsequently, an approach utilizing PTs with four different pendant groups in conjunction with four different Apts is demonstrated for qualitative colorimetric profiling and for quantitative fluorometric profiling of up to four AGEs in clinical matrices. Principal component analysis (PCA) of fluorometric responses of AGE-spiked samples yielded distinct responses for the different AGEs tested. Thus, the proposed approach ascertains rapid profiling of spiked AGEs in plasma samples without the requirement of preanalytical processing and advanced instrumentation, thereby facilitating on-site diagnosis. |
| فهرسة مساهمة: | Keywords: advanced glycation end products (AGEs); aptamers; plasma; point of care (POC); polythiophenes; sensor array |
| المشرفين على المادة: | 0 (Aptamers, Nucleotide) 0 (Biomarkers) 0 (Fluorescent Dyes) 0 (Glycation End Products, Advanced) 0 (Polymers) 0 (Thiophenes) 25233-34-5 (polythiophene) |
| تواريخ الأحداث: | Date Created: 20211229 Date Completed: 20220204 Latest Revision: 20220204 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1021/acsami.1c16261 |
| PMID: | 34964349 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1944-8252 |
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| DOI: | 10.1021/acsami.1c16261 |