دورية أكاديمية
Crystal structure of Tam41 cytidine diphosphate diacylglycerol synthase from a Firmicutes bacterium.
| العنوان: | Crystal structure of Tam41 cytidine diphosphate diacylglycerol synthase from a Firmicutes bacterium. |
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| المؤلفون: | Kimura K; Graduate School of Global Symbiotic Sciences, Yamagata University, 1-4-12 Kojirakawa-machi, Yamagata 990-8560, Japan., Kawai F; Faculty of Science, Yamagata University, 1-4-12 Kojirakawa-machi, Yamagata 990-8560, Japan., Kubota-Kawai H; Faculty of Science, Yamagata University, 1-4-12 Kojirakawa-machi, Yamagata 990-8560, Japan., Watanabe Y; Faculty of Science, Yamagata University, 1-4-12 Kojirakawa-machi, Yamagata 990-8560, Japan., Tomii K; Artificial Intelligence Research Center (AIRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan., Kojima R; Faculty of Science, Yamagata University, 1-4-12 Kojirakawa-machi, Yamagata 990-8560, Japan.; Research Center for Drug Development and Quality Control, Toyama Prefectural Institute for Pharmaceutical Research, 17-1, Nakataikouyama, Imizu, Toyama 939-0363, Japan., Hirata K; Advanced Photon Technology Division, Research Infrastructure Group, SR Life Science Instrumentation Unit, RIKEN/SPring-8 Center, Hyogo 679-5148, Japan., Yamamori Y; Artificial Intelligence Research Center (AIRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan., Endo T; Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-motoyama, Kyoto, 603-8555, Japan.; Institute for Protein Dynamics, Kyoto Sangyo University, Kamigamo-motoyama, Kyoto, 603-8555, Japan., Tamura Y; Faculty of Science, Yamagata University, 1-4-12 Kojirakawa-machi, Yamagata 990-8560, Japan. |
| المصدر: | Journal of biochemistry [J Biochem] 2022 Mar 31; Vol. 171 (4), pp. 429-441. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0376600 Publication Model: Print Cited Medium: Internet ISSN: 1756-2651 (Electronic) Linking ISSN: 0021924X NLM ISO Abbreviation: J Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: Tokyo : Japanese Biochemical Society |
| مواضيع طبية MeSH: | Cytidine Diphosphate* , Diacylglycerol Cholinephosphotransferase*/genetics , Diacylglycerol Cholinephosphotransferase*/metabolism, Cardiolipins ; Diglycerides ; Firmicutes/metabolism |
| مستخلص: | Translocator assembly and maintenance 41 (Tam41) catalyses the synthesis of cytidine diphosphate diacylglycerol (CDP-DAG), which is a high-energy intermediate phospholipid critical for generating cardiolipin in mitochondria. Although Tam41 is present almost exclusively in eukaryotic cells, a Firmicutes bacterium contains the gene encoding Tam41-type CDP-DAG synthase (FbTam41). FbTam41 converted phosphatidic acid (PA) to CDP-DAG using a ternary complex mechanism in vitro. Additionally, FbTam41 functionally substituted yeast Tam41 in vivo. These results demonstrate that Tam41-type CDP-DAG synthase functions in some prokaryotic cells. We determined the crystal structure of FbTam41 lacking the C-terminal 18 residues in the cytidine triphosphate (CTP)-Mg2+ bound form at a resolution of 2.6 Å. The crystal structure showed that FbTam41 contained a positively charged pocket that specifically accommodated CTP-Mg2+ and PA in close proximity. By using this structure, we constructed a model for the full-length structure of FbTam41 containing the last a-helix, which was missing in the crystal structure. Based on this model, we propose a molecular mechanism for CDP-DAG synthesis in bacterial cells and mitochondria. (© The Author(s) 2022. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Firmicutes bacterium; Tam41; cardiolipin; mitochondria; phospholipid |
| المشرفين على المادة: | 0 (Cardiolipins) 0 (Diglycerides) 63-38-7 (Cytidine Diphosphate) EC 2.7.8.2 (Diacylglycerol Cholinephosphotransferase) |
| تواريخ الأحداث: | Date Created: 20211229 Date Completed: 20220404 Latest Revision: 20220405 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/jb/mvab154 |
| PMID: | 34964897 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1756-2651 |
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| DOI: | 10.1093/jb/mvab154 |