دورية أكاديمية
أعرض في EDS

(-)-α-Bisabolol as a protective agent against epithelial renal cytotoxicity induced by amphotericin B.

التفاصيل البيبلوغرافية
العنوان: (-)-α-Bisabolol as a protective agent against epithelial renal cytotoxicity induced by amphotericin B.
المؤلفون: Magalhães EP; Postgraduate Program in Pharmaceutical Sciences, Federal University of Ceará, Fortaleza, CE, Brazil., Silva BP; Postgraduate Program in Pharmaceutical Sciences, Federal University of Ceará, Fortaleza, CE, Brazil., Aires NL; Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, CE, Brazil., Ribeiro LR; Postgraduate Program in Pharmaceutical Sciences, Federal University of Ceará, Fortaleza, CE, Brazil., Ali A; Postgraduate Program in Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Cavalcanti MM; Postgraduate Program in Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Nunes JVS; Postgraduate Program in Pharmaceutical Sciences, Federal University of Ceará, Fortaleza, CE, Brazil., Sampaio TL; Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, CE, Brazil., de Menezes RRPPB; Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, CE, Brazil. Electronic address: ramonppessoa@ufc.br., Martins AMC; Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, CE, Brazil.
المصدر: Life sciences [Life Sci] 2022 Feb 15; Vol. 291, pp. 120271. Date of Electronic Publication: 2021 Dec 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: <2008->: Amsterdam : Elsevier
Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press.
مواضيع طبية MeSH: Cell Survival/*drug effects , Kidney Tubules, Proximal/*drug effects , Monocyclic Sesquiterpenes/*pharmacology, Amphotericin B/pharmacology ; Amphotericin B/toxicity ; Animals ; Antifungal Agents/pharmacology ; Antioxidants/pharmacology ; Apoptosis/drug effects ; Cell Line ; Hepatitis A Virus Cellular Receptor 1/metabolism ; Kidney/metabolism ; Kidney Tubules, Proximal/metabolism ; Macaca mulatta ; Monocyclic Sesquiterpenes/metabolism ; Oxidative Stress/drug effects ; Protective Agents/pharmacology
مستخلص: Introduction: Amphotericin B (AmB), used for systemic fungal infections, has a limited clinical application because of its high nephrotoxicity. Natural antioxidant and anti-inflammatory substances have been widely studied for protection against drug-induced nephrotoxicity. α-Bisabolol (BIS) has demonstrated a nephroprotective effect on both in vitro and in vivo models.
Aims: The aim of this work was to evaluate the effect of BIS against AmB-induced nephrotoxicity in vitro.
Material and Methods: LLC-MK2 cells were pre- and post-treated with non-toxic BIS concentrations and/or AmB IC50 (13.97 μM). Cell viability was assessed by MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] assay. Flow cytometry analyses were used to assess cell death mechanism, production of reactive oxidative stress (ROS) and mitochondrial transmembrane potential. Kidney Injury Molecule-1 (KIM-1) levels were measured via ELISA.
Key Findings: The present work showed that BIS pretreatment (125; 62.5 and 31.25 μM) increased cell viability when compared to the group treated only with AmB IC50. AmB treatment induced both necrosis (7-AAD-labeled cells) and late apoptosis (AnxV-labeled). BIS was able to prevent the occurrence of these events. These effects were associated with a decrease of ROS accumulation, improving transmembrane mitochondrial potential and protecting against tubular cell damage, highlighted by the inhibition of KIM-1 release after BIS treatment.
Significance: BIS presented a potential effect on model of renal cytotoxicity induced by AmB, bringing perspectives for the research of new nephroprotective agents.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: KIM-1; Natural products; Necrosis; Nephrotoxicity; Oxidative stress; Terpenes
المشرفين على المادة: 0 (Antifungal Agents)
0 (Antioxidants)
0 (Hepatitis A Virus Cellular Receptor 1)
0 (Monocyclic Sesquiterpenes)
0 (Protective Agents)
24WE03BX2T (bisabolol)
7XU7A7DROE (Amphotericin B)
تواريخ الأحداث: Date Created: 20220102 Date Completed: 20220217 Latest Revision: 20220217
رمز التحديث: 20240628
DOI: 10.1016/j.lfs.2021.120271
PMID: 34974077
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0631
DOI:10.1016/j.lfs.2021.120271