Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimen.
| العنوان: | Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimen. |
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| المؤلفون: | Nayrac M; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Dubé M; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Sannier G; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Nicolas A; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Marchitto L; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Tastet O; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Tauzin A; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Brassard N; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Beaudoin-Bussières G; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Vézina D; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Gong SY; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Department of Microbiology and Immunology, McGill University, Montreal, QC, H3A 2B4, Canada., Benlarbi M; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Gasser R; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Laumaea A; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Bourassa C; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Gendron-Lepage G; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Medjahed H; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Goyette G; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Ortega-Delgado GG; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Laporte M; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Niessl J; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada.; Consortium for HIV/AIDS Vaccine Development (CHAVD), La Jolla, CA, USA., Gokool L; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Morrisseau C; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Arlotto P; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada., Richard J; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Tremblay C; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Martel-Laferrière V; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada., Finzi A; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, H2X 0A9, Canada.; Department of Microbiology and Immunology, McGill University, Montreal, QC, H3A 2B4, Canada., Kaufmann DE; Centre de Recherche du CHUM, Montréal, QC, H2X 0A9 Canada.; Consortium for HIV/AIDS Vaccine Development (CHAVD), La Jolla, CA, USA.; Département de Médecine, Université de Montréal, Montreal, QC, H3T 1J4, Canada. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2021 Dec 21. Date of Electronic Publication: 2021 Dec 21. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Spacing of the BNT162b2 mRNA doses beyond 3 weeks raised concerns about vaccine efficacy. We longitudinally analyzed B cell, T cell and humoral responses to two BNT162b2 mRNA doses administered 16 weeks apart in 53 SARS-CoV-2 naïve and previously-infected donors. This regimen elicited robust RBD-specific B cell responses whose kinetics differed between cohorts, the second dose leading to increased magnitude in naïve participants only. While boosting did not increase magnitude of CD4 + T cell responses further compared to the first dose, unsupervised clustering analyses of single-cell features revealed phenotypic and functional shifts over time and between cohorts. Integrated analysis showed longitudinal immune component-specific associations, with early Thelper responses post-first dose correlating with B cell responses after the second dose, and memory Thelper generated between doses correlating with CD8 T cell responses after boosting. Therefore, boosting elicits a robust cellular recall response after the 16-week interval, indicating functional immune memory. |
| التعليقات: | Update in: Cell Rep. 2022 Jun 28;39(13):111013. (PMID: 35732172) |
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| معلومات مُعتمدة: | UM1 AI144462 United States AI NIAID NIH HHS |
| تواريخ الأحداث: | Date Created: 20220104 Latest Revision: 20240405 |
| رمز التحديث: | 20240405 |
| مُعرف محوري في PubMed: | PMC8722583 |
| DOI: | 10.1101/2021.12.18.473317 |
| PMID: | 34981046 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2021.12.18.473317 |
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