دورية أكاديمية
Rewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transection.
| العنوان: | Rewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transection. |
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| المؤلفون: | Nogueira-Rodrigues J; Nerve Regeneration Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Graduate Program in Molecular and Cell Biology, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal., Leite SC; Nerve Regeneration Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal., Pinto-Costa R; Nerve Regeneration Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal., Sousa SC; Nerve Regeneration Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Graduate Program in Molecular and Cell Biology, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal., Luz LL; Neuronal Networks Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal., Sintra MA; Nerve Regeneration Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal., Oliveira R; Translational NeuroUrology Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Department of Biomedicine, Experimental Biology Unit, Faculty of Medicine of Porto, University of Porto, 4200-319 Porto, Portugal; Regeneration Group, Wolfson Centre for Age-Related Diseases, Institute of Psychiatry, Psychology and Neuroscience, King's College London WC2R 2LS, London, UK., Monteiro AC; Nerve Regeneration Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal., Pinheiro GG; Molecular & Regenerative Medicine Laboratory, Centro de Ciências do Mar (CCMAR), University of Algarve, 8005-139 Faro, Portugal; Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal., Vitorino M; Molecular & Regenerative Medicine Laboratory, Centro de Ciências do Mar (CCMAR), University of Algarve, 8005-139 Faro, Portugal; Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal., Silva JA; Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal., Simão S; Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal; Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, 8005-139 Faro, Portugal., Fernandes VE; Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal; Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, 8005-139 Faro, Portugal., Provazník J; Genomics Core Facility, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany., Benes V; Genomics Core Facility, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany., Cruz CD; Translational NeuroUrology Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Department of Biomedicine, Experimental Biology Unit, Faculty of Medicine of Porto, University of Porto, 4200-319 Porto, Portugal., Safronov BV; Neuronal Networks Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal., Magalhães A; Glycobiology in Cancer Group, Institute of Molecular Pathology and Immunology, IPATIMUP), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Department of Molecular Biology, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal., Reis CA; Glycobiology in Cancer Group, Institute of Molecular Pathology and Immunology, IPATIMUP), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Department of Molecular Biology, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal; Department of Pathology, Faculty of Medicine of Porto, University of Porto, 4200-319 Porto, Portugal., Vieira J; Phenotypic Evolution Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal., Vieira CP; Phenotypic Evolution Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal., Tiscórnia G; Molecular & Regenerative Medicine Laboratory, Centro de Ciências do Mar (CCMAR), University of Algarve, 8005-139 Faro, Portugal; Clinica Eugin, Research and Development, 08006 Barcelona, Spain., Araújo IM; Faculty of Medicine and Biomedical Sciences, University of Algarve, 8005-139 Faro, Portugal; Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, 8005-139 Faro, Portugal; Champalimaud Research Program, Champalimaud Center for the Unknown, 1400-038 Lisbon, Portugal., Sousa MM; Nerve Regeneration Group, Instituto de Biologia Molecular e Celular (IBMC), Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal. Electronic address: msousa@ibmc.up.pt. |
| المصدر: | Developmental cell [Dev Cell] 2022 Feb 28; Vol. 57 (4), pp. 440-450.e7. Date of Electronic Publication: 2022 Jan 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101120028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1551 (Electronic) Linking ISSN: 15345807 NLM ISO Abbreviation: Dev Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, Mass. : Cell Press, c2001- |
| مواضيع طبية MeSH: | Axons/*physiology , Nerve Regeneration/*physiology , Recovery of Function/*physiology , Spinal Cord Injuries/*pathology, Animals ; Axons/pathology ; Disease Models, Animal ; Glycosylation ; Mice ; Spinal Cord/physiology ; Spinal Cord/physiopathology ; Spinal Cord Injuries/physiopathology ; Spine/physiopathology |
| مستخلص: | Regeneration of adult mammalian central nervous system (CNS) axons is abortive, resulting in inability to recover function after CNS lesion, including spinal cord injury (SCI). Here, we show that the spiny mouse (Acomys) is an exception to other mammals, being capable of spontaneous and fast restoration of function after severe SCI, re-establishing hind limb coordination. Remarkably, Acomys assembles a scarless pro-regenerative tissue at the injury site, providing a unique structural continuity of the initial spinal cord geometry. The Acomys SCI site shows robust axon regeneration of multiple tracts, synapse formation, and electrophysiological signal propagation. Transcriptomic analysis of the spinal cord following transcriptome reconstruction revealed that Acomys rewires glycosylation biosynthetic pathways, culminating in a specific pro-regenerative proteoglycan signature at SCI site. Our work uncovers that a glycosylation switch is critical for axon regeneration after SCI and identifies β3gnt7, a crucial enzyme of keratan sulfate biosynthesis, as an enhancer of axon growth. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| التعليقات: | Comment in: Nat Rev Neurosci. 2022 Mar;23(3):131. (PMID: 35091699) Comment in: Dev Cell. 2022 Feb 28;57(4):415-416. (PMID: 35231422) |
| فهرسة مساهمة: | Keywords: Acomys cahirinus; acetylglucosaminyltransferase; axon regeneration; fibrotic scar; glial scar; glycosaminoglycan; glycosylation; proteoglycan; spinal cord injury; spiny mouse |
| تواريخ الأحداث: | Date Created: 20220105 Date Completed: 20220311 Latest Revision: 20220321 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.devcel.2021.12.008 |
| PMID: | 34986324 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1878-1551 |
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| DOI: | 10.1016/j.devcel.2021.12.008 |