دورية أكاديمية
Atto 465 Derivative Is a Nuclear Stain with Unique Excitation and Emission Spectra Useful for Multiplex Immunofluorescence Histochemistry.
| العنوان: | Atto 465 Derivative Is a Nuclear Stain with Unique Excitation and Emission Spectra Useful for Multiplex Immunofluorescence Histochemistry. |
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| المؤلفون: | Dodge JT; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland., Doyle AD; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland., Costa-da-Silva AC; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland., Hogden CT; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland., Mezey E; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland., Mays JW; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland. |
| المصدر: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society [J Histochem Cytochem] 2022 Mar; Vol. 70 (3), pp. 211-223. Date of Electronic Publication: 2022 Jan 07. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Intramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: SAGE Publications Country of Publication: United States NLM ID: 9815334 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1551-5044 (Electronic) Linking ISSN: 00221554 NLM ISO Abbreviation: J Histochem Cytochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2011->: Thousand Oaks, CA : SAGE Publications Original Publication: Baltimore : Williams & Wilkins Co., c1953- |
| مواضيع طبية MeSH: | Cell Nucleus/*chemistry , Proflavine/*analogs & derivatives, Aniline Compounds/chemistry ; Animals ; Female ; Fluorescent Antibody Technique ; Fluorescent Dyes/chemistry ; Fluorobenzenes/chemistry ; Fluorocarbons/chemistry ; Histocytochemistry ; Mice ; Mice, Inbred BALB C ; Proflavine/analysis |
| مستخلص: | Multiplex immunofluorescence (mIF) is an effective technique for the maximal visualization of multiple target proteins in situ. This powerful tool is mainly limited by the spectral overlap of the currently available synthetic fluorescent dyes. The fluorescence excitation wavelengths ranging between 405 and 488 nm are rarely used in mIF imaging and serve as a logical additional slot for a fluorescent probe. In the present study, we demonstrate that the addition of 2,3,4,5,6-pentafluoroaniline to Atto 465 NHS ester, creating Atto 465-pentafluoroaniline (Atto 465-p), generates a bright nuclear stain in the violet-blue region of the visible spectrum. This allows the 405 nm excitation and emission, classically used for nuclear counterstains, to be used for the detection of another target protein. This increases the flexibility of the mIF panel and, with appropriate staining and microscopy, enables the quantitative analysis of at least six targets in one tissue section. (J Histochem Cytochem XX: XXX-XXX, XXXX) . |
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| معلومات مُعتمدة: | ZIA DE000714 United States ImNIH Intramural NIH HHS; ZIA DE000747 United States ImNIH Intramural NIH HHS; ZIC DE000750 United States ImNIH Intramural NIH HHS |
| فهرسة مساهمة: | Keywords: Atto 465; SP8; immunofluorescence; immunohistochemistry; multiplex; nuclear stain; photoconversion; photostability; proflavine; tyramide |
| المشرفين على المادة: | 0 (ATTO 465) 0 (Aniline Compounds) 0 (Fluorescent Dyes) 0 (Fluorobenzenes) 0 (Fluorocarbons) 771-60-8 (2,3,4,5,6-pentafluoroaniline) CY3RNB3K4T (Proflavine) |
| تواريخ الأحداث: | Date Created: 20220107 Date Completed: 20220221 Latest Revision: 20230302 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8832627 |
| DOI: | 10.1369/00221554211064942 |
| PMID: | 34994225 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1551-5044 |
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| DOI: | 10.1369/00221554211064942 |