دورية أكاديمية
Building an embryo: An auxin gene toolkit for zygotic and somatic embryogenesis in Brazilian pine.
| العنوان: | Building an embryo: An auxin gene toolkit for zygotic and somatic embryogenesis in Brazilian pine. |
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| المؤلفون: | Elbl PM; Laboratory of Plant Cell Biology, Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão, 277, São Paulo, SP, Brazil. Electronic address: paulaelbl@gmail.com., de Souza DT; Laboratory of Plant Cell Biology, Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão, 277, São Paulo, SP, Brazil; Department of Computer Science, Institute of Mathematics and Statistics, University of São Paulo, São Paulo, SP, Brazil., Rosado D; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, United States., de Oliveira LF; Laboratory of Plant Cell Biology, Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão, 277, São Paulo, SP, Brazil., Navarro BV; Laboratory of Plant Cell Biology, Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão, 277, São Paulo, SP, Brazil; Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, SP, Brazil., Matioli SR; Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, SP, Brazil., Floh EIS; Laboratory of Plant Cell Biology, Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão, 277, São Paulo, SP, Brazil. |
| المصدر: | Gene [Gene] 2022 Apr 05; Vol. 817, pp. 146168. Date of Electronic Publication: 2022 Jan 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Plant Somatic Embryogenesis Techniques*, Araucaria/*embryology , Araucaria/*genetics , Indoleacetic Acids/*metabolism , Seeds/*growth & development, Arabidopsis/genetics ; Body Patterning ; Evolution, Molecular ; Phylogeny ; Plant Development/genetics |
| مستخلص: | Many studies in the model species Arabidopsis thaliana characterized genes involved in embryo formation. However, much remains to be learned about the portfolio of genes that are involved in signal transduction and transcriptional regulation during plant embryo development in other species, particularly in an evolutionary context, especially considering that some genes involved in embryo patterning are not exclusive of land plants. This study, used a combination of domain architecture phylostratigraphy and phylogenetic reconstruction to investigate the evolutionary history of embryo patterning and auxin metabolism (EPAM) genes in Viridiplantae. This approach shed light on the co-optation of auxin metabolism and other molecular mechanisms that contributed to the radiation of land plants, and specifically to embryo formation. These results have potential to assist conservation programs, by directing the development of tools for obtaining somatic embryos. In this context, we employed this methodology with critically endangered and non-model species Araucaria angustifolia, the Brazilian pine, which is current focus of conservation efforts using somatic embryogenesis. So far, this approach had little success since somatic embryos fail to completely develop. By profiling the expression of genes that we identified as necessary for the emergence of land-plant embryos, we found striking differences between zygotic and somatic embryos that might explain the developmental arrest and be used to improve A. angustifolia somatic culture. (Copyright © 2021. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Auxin; Conifers; Conservation; Embryogenesis; Phylogenetic reconstruction; Phylostratigraphy |
| المشرفين على المادة: | 0 (Indoleacetic Acids) |
| تواريخ الأحداث: | Date Created: 20220107 Date Completed: 20220223 Latest Revision: 20220223 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.gene.2021.146168 |
| PMID: | 34995731 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0038 |
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| DOI: | 10.1016/j.gene.2021.146168 |