دورية أكاديمية
A competitive assay for the detection of a 16-mer peptide from α1 chain of human collagen XI.
| العنوان: | A competitive assay for the detection of a 16-mer peptide from α1 chain of human collagen XI. |
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| المؤلفون: | Lorenzo-Gómez R; Departamento de Química Física y Analítica. Facultad de Química, Universidad de Oviedo, Av. Julián Clavería 8, 33006, Oviedo, Spain; Instituto de Investigación Sanitaria del Principado de Asturias, Av. de Roma s/n, 33011, Oviedo, Spain. Electronic address: lorenzoramon@uniovi.es., Casero-Álvarez A; Departamento de Química Física y Analítica. Facultad de Química, Universidad de Oviedo, Av. Julián Clavería 8, 33006, Oviedo, Spain. Electronic address: uo246417@uniovi.es., Miranda-Castro R; Departamento de Química Física y Analítica. Facultad de Química, Universidad de Oviedo, Av. Julián Clavería 8, 33006, Oviedo, Spain; Instituto de Investigación Sanitaria del Principado de Asturias, Av. de Roma s/n, 33011, Oviedo, Spain. Electronic address: mirandarebeca@uniovi.es., García-Ocaña M; Unidad de Biotecnología y Ensayos Biomédicos. Servicios Científico Técnicos, Universidad de Oviedo, Oviedo, Spain. Electronic address: garciaomarcos@uniovi.es., de Los Toyos JR; Instituto de Investigación Sanitaria del Principado de Asturias, Av. de Roma s/n, 33011, Oviedo, Spain; Área de Inmunología, Facultad de Medicina y Ciencias de la Salud, Universidad de Oviedo, Av. Julián Clavería 6, 33006, Oviedo, Spain. Electronic address: jrtoyos@uniovi.es., de-Los-Santos-Alvarez N; Departamento de Química Física y Analítica. Facultad de Química, Universidad de Oviedo, Av. Julián Clavería 8, 33006, Oviedo, Spain; Instituto de Investigación Sanitaria del Principado de Asturias, Av. de Roma s/n, 33011, Oviedo, Spain. Electronic address: santosnoemi@uniovi.es., Lobo-Castañón MJ; Departamento de Química Física y Analítica. Facultad de Química, Universidad de Oviedo, Av. Julián Clavería 8, 33006, Oviedo, Spain; Instituto de Investigación Sanitaria del Principado de Asturias, Av. de Roma s/n, 33011, Oviedo, Spain. Electronic address: mjlc@uniovi.es. |
| المصدر: | Talanta [Talanta] 2022 Apr 01; Vol. 240, pp. 123196. Date of Electronic Publication: 2021 Dec 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| مواضيع طبية MeSH: | Collagen* , Neoplasms*, Biomarkers, Tumor ; Extracellular Matrix ; Humans ; Peptides |
| مستخلص: | Characterization of extracellular matrix (ECM) is becoming more and more important to decipher cancer progression. Constant remodeling results in ECM components degradation or unusual ECM accumulation that releases short fragments to the body fluids. These fragments might be potential cancer biomarkers but to detect them specific receptors are needed. In response to this demand, we present the first electrochemical aptamer-based competitive assay for the minor collagen XI, dysregulated in several carcinomas. It was performed on magnetic beads using enzymatic labeling. First, we selected the most appropriate tag for the aptamer (biotin or 6-carboxyfluorescein). The former yielded higher currents by chronoamperometry and it was used for the competitive assay. The collagen fragment, a 16mer peptide used as the target, was detected from 52 to 1000 nM with an RSD of about 5%. The LOD of the assay was estimated as 24 nM (44 ng/mL). The performance of the assay in serum diluted 1:2 was equivalent to the assay in PBS. The detection of α1 chain of human collagen XI was also possible in cell lysates and confirmed by aptacytofluorescence, which is promising as a new tool to validate this fragment as a cancer biomarker. (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 16-Mer telopeptide; Aptamer; Collagen XI alpha 1 chain; ECM component; Electrochemical competitive assay; Neoepitope |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (Peptides) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20220108 Date Completed: 20220127 Latest Revision: 20220127 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.talanta.2021.123196 |
| PMID: | 34998145 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3573 |
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| DOI: | 10.1016/j.talanta.2021.123196 |