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Transcriptome-Wide m6A Analysis Provides Novel Insights Into Testicular Development and Spermatogenesis in Xia-Nan Cattle.

التفاصيل البيبلوغرافية
العنوان: Transcriptome-Wide m6A Analysis Provides Novel Insights Into Testicular Development and Spermatogenesis in Xia-Nan Cattle.
المؤلفون: Liu SH; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Ma XY; Guangxi Provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, China., Yue TT; Henan Dairy Herd Improvement Co., Ltd, Zhengzhou, China., Wang ZC; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Qi KL; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Li JC; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Lin F; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Rushdi HE; Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt., Gao YY; Henan Dingyuan Cattle Breeding Co., Ltd., Wuhan, China., Fu T; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Li M; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Gao TY; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Yang LG; China Ministry of Education, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China., Han XL; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China., Deng TX; Guangxi Provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, China.
المصدر: Frontiers in cell and developmental biology [Front Cell Dev Biol] 2021 Dec 22; Vol. 9, pp. 791221. Date of Electronic Publication: 2021 Dec 22 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101630250 Publication Model: eCollection Cited Medium: Print ISSN: 2296-634X (Print) Linking ISSN: 2296634X NLM ISO Abbreviation: Front Cell Dev Biol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2013]-
مستخلص: Testis is the primary organ of the male reproductive tract in mammals that plays a substantial role in spermatogenesis. Improvement of our knowledge regarding the molecular mechanisms in testicular development and spermatogenesis will be reflected in producing spermatozoa of superior fertility. Evidence showed that N6-Methyladenosine (m6A) plays a dynamic role in post-transcription gene expression regulation and is strongly associated with production traits. However, the role of m6A in bovine testis has not been investigated yet. In this study, we conducted MeRIP-Seq analysis to explore the expression profiles of the m6A and its potential mechanism underlying spermatogenesis in nine bovine testes at three developmental stages (prepuberty, puberty and postpuberty). The experimental animals with triplicate in each stage were chosen based on their semen volume and sperm motility except for the prepuberty bulls and used for testes collection. By applying MeRIP-Seq analysis, a total of 8,774 m6A peaks and 6,206 m6A genes among the studied groups were identified. All the detected peaks were found to be mainly enriched in the coding region and 3'- untranslated regions. The cross-analysis of m6A and mRNA expression exhibited 502 genes with concomitant changes in the mRNA expression and m6A modification. Notably, 30 candidate genes were located in the largest network of protein-protein interactions. Interestingly, four key node genes ( PLK4, PTEN, EGR1 , and PSME4 ) were associated with the regulation of mammal testis development and spermatogenesis. This study is the first to present a map of RNA m6A modification in bovine testes at distinct ages, and provides new insights into m6A topology and related molecular mechanisms underlying bovine spermatogenesis, and establishes a basis for further studies on spermatogenesis in mammals.
Competing Interests: T-tY was employed by Henan Dairy Herd Improvement Co., Ltd., Y-yG was employed by Henan Dingyuan Cattle Breeding Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Liu, Ma, Yue, Wang, Qi, Li, Lin, Rushdi, Gao, Fu, Li, Gao, Yang, Han and Deng.)
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فهرسة مساهمة: Keywords: cattle; m6A modification; semen quality; sequencing; spermatogenesis; testis
تواريخ الأحداث: Date Created: 20220110 Latest Revision: 20231105
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8728086
DOI: 10.3389/fcell.2021.791221
PMID: 35004687
قاعدة البيانات: MEDLINE
الوصف
تدمد:2296-634X
DOI:10.3389/fcell.2021.791221