دورية أكاديمية
أعرض في EDS

Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.

التفاصيل البيبلوغرافية
العنوان: Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
المؤلفون: Facchetti D; Engineering Department, Lancaster University, Lancaster LA1 4YW, UK.; Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London W12 0BZ, UK., Hempel U; Institute of Physiological Chemistry, Technische Universität Dresden, 01307 Dresden, Germany., Martocq L; Engineering Department, Lancaster University, Lancaster LA1 4YW, UK., Smith AM; Department of Pharmacy, School of Applied Sciences, University of Huddersfield, Huddersfield HD1 3DR, UK., Koptyug A; Department of Quality Technology, Mechanical Engineering & Mathematics, Mid Sweden University, 831 25 Ostersund, Sweden., Surmenev RA; Physical Materials Science and Composite Materials Centre, Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.; Piezo- and Magnetoelectric Materials Research & Development Centre, Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia., Surmeneva MA; Physical Materials Science and Composite Materials Centre, Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.; Piezo- and Magnetoelectric Materials Research & Development Centre, Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia., Douglas TEL; Engineering Department, Lancaster University, Lancaster LA1 4YW, UK.; Materials Science Institute (MSI), Lancaster University, Lancaster, UK.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2021 Dec 23; Vol. 23 (1). Date of Electronic Publication: 2021 Dec 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Alloys/*pharmacology , Cell Differentiation/*drug effects , Cell Proliferation/*drug effects , Heparin/*pharmacology , Hydrophobic and Hydrophilic Interactions/*drug effects , Mesenchymal Stem Cells/*drug effects , Titanium/*pharmacology , Whey Proteins/*pharmacology, Adult ; Alkaline Phosphatase/metabolism ; Biocompatible Materials/pharmacology ; Bone and Bones/drug effects ; Bone and Bones/metabolism ; Cells, Cultured ; Coated Materials, Biocompatible/pharmacology ; Humans ; Male ; Mesenchymal Stem Cells/metabolism ; Osteogenesis/drug effects
مستخلص: Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion. The success of Ti6Al4V implants depends on bone formation on the implant surface. Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable. One coating strategy is by adsorption of biomacromolecules. In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells. WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC). Indeed, WPI fibrils were resistant to sterilization and were stable during storage. This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity. Finally, the coating increased the hydrophilicity of the material. The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments. Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
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فهرسة مساهمة: Keywords: Ti6Al4V; WPI fibrils; additive manufacturing; coating; enriched; heparin; osseointegration; osteoblast differentiation; tinzaparin
المشرفين على المادة: 0 (Alloys)
0 (Biocompatible Materials)
0 (Coated Materials, Biocompatible)
0 (Whey Proteins)
12743-70-3 (titanium alloy (TiAl6V4))
9005-49-6 (Heparin)
D1JT611TNE (Titanium)
EC 3.1.3.1 (Alkaline Phosphatase)
تواريخ الأحداث: Date Created: 20220111 Date Completed: 20220201 Latest Revision: 20220201
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8745389
DOI: 10.3390/ijms23010139
PMID: 35008562
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms23010139