دورية أكاديمية
أعرض في EDS

Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins.

التفاصيل البيبلوغرافية
العنوان: Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins.
المؤلفون: Zhang D; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore., Wang Z; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore., Hu S; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore., Lescar J; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore., Tam JP; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore., Liu CF; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2021 Dec 31; Vol. 23 (1). Date of Electronic Publication: 2021 Dec 31.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Ligases*/chemistry , Ligases*/metabolism, Biotechnology/*methods , Proteins/*metabolism, Humans ; MCF-7 Cells ; Protein Processing, Post-Translational
مستخلص: The last two decades have seen an increasing demand for new protein-modification methods from the biotech industry and biomedical research communities. Owing to their mild aqueous reaction conditions, enzymatic methods based on the use of peptide ligases are particularly desirable. In this regard, the recently discovered peptidyl Asx-specific ligases (PALs) have emerged as powerful biotechnological tools in recent years. However, as a new class of peptide ligases, their scope and application remain underexplored. Herein, we report the use of a new PAL, VyPAL2, for a diverse range of protein modifications. We successfully showed that VyPAL2 was an efficient biocatalyst for protein labelling, inter-protein ligation, and protein cyclization. The labelled or cyclized protein ligands remained functionally active in binding to their target receptors. We also demonstrated on-cell labelling of protein ligands pre-bound to cellular receptors and cell-surface engineering via modifying a covalently anchored peptide substrate pre-installed on cell-surface glycans. Together, these examples firmly establish Asx-specific ligases, such as VyPAL2, as the biocatalysts of the future for site-specific protein modification, with a myriad of applications in basic research and drug discovery.
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معلومات مُعتمدة: MOE2016-T3-1-003; 2019-T1-002-100 Ministry of Education
فهرسة مساهمة: Keywords: VyPAL2; biocatalysts; cell surface labeling; protein cyclization; protein labeling
المشرفين على المادة: 0 (Proteins)
EC 6.- (Ligases)
تواريخ الأحداث: Date Created: 20220111 Date Completed: 20220211 Latest Revision: 20220211
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8745061
DOI: 10.3390/ijms23010458
PMID: 35008882
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms23010458