دورية أكاديمية
Diagnosis, risk factors analysis and first molecular characterization of Cryptosporidium spp. in horses from Rio de Janeiro, Brazil.
| العنوان: | Diagnosis, risk factors analysis and first molecular characterization of Cryptosporidium spp. in horses from Rio de Janeiro, Brazil. |
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| المؤلفون: | Ribeiro DSC; Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói, Rio de Janeiro 24210130, Brazil., Martins AV; Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói, Rio de Janeiro 24210130, Brazil; Centro Universitário Serra dos Órgãos, Faculdade de Medicina Veterinária, Campus Quinta do Paraíso, Estrada Wenceslau José de Medeiros, 1045, Prata, Teresópolis, Rio de Janeiro, 25976-340, Brazil., Lobão LF; Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói, Rio de Janeiro 24210130, Brazil., Ribeiro MS; Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói, Rio de Janeiro 24210130, Brazil., Palmer JPS; Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói, Rio de Janeiro 24210130, Brazil., Corrêa LL; Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói, Rio de Janeiro 24210130, Brazil., Uchôa CMA; Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói, Rio de Janeiro 24210130, Brazil., da Silva S; Fundação Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Parasitologia, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro 21045900, Brazil., Meireles MV; Universidade Estadual Paulista, Campus Araçatuba, Rua Clóvis Pestana, 793, Bairro Dona Amélia, CEP 16050-680, Araçatuba, SP, Brazil., Amendoeira MRR; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Toxoplasmose e outras Protozoooses, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro 21045900, Brazil., Barbosa ADS; Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Rua Hernani de Mello, 101, São Domingos, Niterói, Rio de Janeiro 24210130, Brazil; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Toxoplasmose e outras Protozoooses, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro 21045900, Brazil. Electronic address: alynnedsb@gmail.com. |
| المصدر: | Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2022 Jan; Vol. 27, pp. 100665. Date of Electronic Publication: 2021 Nov 25. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101680410 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2405-9390 (Electronic) Linking ISSN: 24059390 NLM ISO Abbreviation: Vet Parasitol Reg Stud Reports Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier, [2015]- |
| مواضيع طبية MeSH: | Cryptosporidiosis*/diagnosis , Cryptosporidiosis*/epidemiology , Cryptosporidium*/genetics , Cryptosporidium parvum*/genetics , Horse Diseases*/diagnosis , Horse Diseases*/epidemiology, Animals ; Brazil/epidemiology ; Horses ; Risk Factors ; United States |
| مستخلص: | An analysis was made of the frequency of Cryptosporidium spp. in fecal samples from horses raised on farms in the Teresópolis city, state of Rio de Janeiro, Brazil, and the risk factors that favored this infection. Between 2019 and 2020, 314 samples of equine feces were collected, 287 of which came from English Thoroughbred horses and 27 from ponies. Information on the horses and their management were retrieved from a stud book and forms filled out by trainers. The fecal samples were subjected to macroscopic analysis, modified Sheather's and Lutz parasitological techniques, safranin staining, and to enzyme-linked immunosorbent assay (ELISA) for the detection of coproantigens. All the samples that tested positive by these techniques underwent partial sequence analysis of the 18S rRNA gene to characterize the protozoan species. Cryptosporidium spp. was identified in 35 (11.1%) of the samples, 34 from English Thoroughbred horses and one from a pony. Based on a logistic regression model, it was found that the presence of dogs and small ruminants on the farms, and drinking water from a spring, were significantly associated with the animals' infection by the protozoan (p < 0.05). Eight of the English Thoroughbred horse samples underwent molecular characterization, which revealed the presence of Cryptosporidium felis in one sample and Cryptosporidium parvum in seven. The seven samples containing C. parvum were subjected to gp60 gene analysis, based on which nucleotide sequences typical of the IIa family were identified, which are usually transmitted from animals to humans. In addition, the genotype IIaA15G2R1, which is considered to have the highest profile of zoonotic transmissibility, was identified in one Thoroughbred horse. This is the first study conducted in the state of Rio de Janeiro that molecularly characterized Cryptosporidium spp. in horses, and the first on the American continent to detect C. felis in the feces of these animals. (Copyright © 2021. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Cryptosporidiosis; Gastrointestinal protozoan; Horses; Rio de Janeiro |
| تواريخ الأحداث: | Date Created: 20220111 Date Completed: 20220228 Latest Revision: 20220531 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.vprsr.2021.100665 |
| PMID: | 35012721 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2405-9390 |
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| DOI: | 10.1016/j.vprsr.2021.100665 |