دورية أكاديمية
أعرض في EDS

β-Aminoisobutyric acid, L-BAIBA, protects PC12 cells from hydrogen peroxide-induced oxidative stress and apoptosis via activation of the AMPK and PI3K/Akt pathway.

التفاصيل البيبلوغرافية
العنوان: β-Aminoisobutyric acid, L-BAIBA, protects PC12 cells from hydrogen peroxide-induced oxidative stress and apoptosis via activation of the AMPK and PI3K/Akt pathway.
المؤلفون: Minato T; Department of Clinical Laboratory, Aichi Gakuin University Dental Hospital, Nagoya, Japan., Nakamura N; Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan., Saiki T; Department of Pharmacy, Aichi Gakuin University Dental Hospital, Nagoya, Japan., Miyabe M; Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan., Ito M; Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan., Matsubara T; Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan., Naruse K; Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
المصدر: IBRO neuroscience reports [IBRO Neurosci Rep] 2021 Dec 07; Vol. 12, pp. 65-72. Date of Electronic Publication: 2021 Dec 07 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101775148 Publication Model: eCollection Cited Medium: Internet ISSN: 2667-2421 (Electronic) Linking ISSN: 26672421 NLM ISO Abbreviation: IBRO Neurosci Rep Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2021]-
مستخلص: β-Aminoisobutyric acid (BAIBA) is a myokine that is secreted from skeletal muscles by the exercise. Recently, increasing evidence has suggested the multifocal physiological activities of BAIBA. In this study, we investigated whether L-BAIBA has protective effects on rat pheochromocytoma (PC12) cells. Cultured PC12 cells were stimulated with L-BAIBA. Western blot analyses revealed that L-BAIBA stimulation significantly increased the phosphorylation of AMPK and Akt. In contrast, no effect was observed on neurite outgrowth by L-BAIBA. To investigate the effects of L-BAIBA on oxidative stress, PC 12 cells were exposed to hydrogen peroxide (H 2 O 2 ) with and without L-BAIBA. Hydrogen peroxide significantly increased reactive oxygen species (ROS) production and apoptosis in PC12 cells. Pretreatment with L-BAIBA suppressed H 2 O 2 -induced ROS production and apoptosis, which was abolished by the inhibition of AMPK by compound C. On the other hand, the inhibitory effects of L-BAIBA on oxidative stress-induced apoptosis were abolished by the inhibition of both AMPK and PI3K/Akt. In conclusion, we demonstrated that L-BAIBA confers protection against oxidative stress in PC12 cells by activating the AMPK and PI3K/Akt pathways. These results suggest that L-BAIBA may play a crucial role on protection of neuron-like cells and become a pharmacological agent to treat neuronal diseases.
Competing Interests: The authors declare they have no conflict of interest.
(© 2021 The Authors.)
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فهرسة مساهمة: Keywords: AMPK; BAIBA, β-Aminoisobutyric acid; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; FITC, fluorescein isothiocyanate; GPCR, G protein-coupled receptor; Hydrogen peroxide; MRGPRD, Mas-related G protein-coupled receptor type D; Neuron; Oxidative stress; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator-1-alpha; PI3K/Akt; PPARγ, peroxisome proliferator-activated receptor gamma; ROS, reactive oxygen species; TUNEL, TdT-mediated dUTP nick-end labeling; β-Aminoisobutyric acid (BAIBA)
تواريخ الأحداث: Date Created: 20220113 Latest Revision: 20220115
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8724974
DOI: 10.1016/j.ibneur.2021.12.001
PMID: 35024688
قاعدة البيانات: MEDLINE
الوصف
تدمد:2667-2421
DOI:10.1016/j.ibneur.2021.12.001