دورية أكاديمية
Megakaryocyte-stromal cell interactions: Effect on megakaryocyte proliferation, proplatelet production, and survival.
| العنوان: | Megakaryocyte-stromal cell interactions: Effect on megakaryocyte proliferation, proplatelet production, and survival. |
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| المؤلفون: | Goette NP; Institute of Medical Research Alfredo Lanari, University of Buenos Aires, Buenos Aires, Argentina., Borzone FR; Laboratory of Immunohematology, Institute of Biology and Experimental Medicine, National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina., Discianni Lupi AD; Institute of Medical Research Alfredo Lanari, University of Buenos Aires, Buenos Aires, Argentina., Chasseing NA; Laboratory of Immunohematology, Institute of Biology and Experimental Medicine, National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina., Rubio MF; Institute of Medical Research Alfredo Lanari, University of Buenos Aires, Buenos Aires, Argentina; Department of Molecular Biology and Apoptosis, Institute of Medical Research (IDIM), National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina., Costas MA; Institute of Medical Research Alfredo Lanari, University of Buenos Aires, Buenos Aires, Argentina; Department of Molecular Biology and Apoptosis, Institute of Medical Research (IDIM), National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina., Heller PG; Institute of Medical Research Alfredo Lanari, University of Buenos Aires, Buenos Aires, Argentina; Department of Experimental Hematology, Institute of Medical Research (IDIM), National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina., Marta RF; Institute of Medical Research Alfredo Lanari, University of Buenos Aires, Buenos Aires, Argentina; Department of Experimental Hematology, Institute of Medical Research (IDIM), National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina., Lev PR; Institute of Medical Research Alfredo Lanari, University of Buenos Aires, Buenos Aires, Argentina; Department of Experimental Hematology, Institute of Medical Research (IDIM), National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina. Electronic address: paolal2002@gmail.com. |
| المصدر: | Experimental hematology [Exp Hematol] 2022 Mar; Vol. 107, pp. 24-37. Date of Electronic Publication: 2022 Jan 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Inc Country of Publication: Netherlands NLM ID: 0402313 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2399 (Electronic) Linking ISSN: 0301472X NLM ISO Abbreviation: Exp Hematol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : Amsterdam : Elsevier Science Inc. Original Publication: Copenhagen, Munksgaard. |
| مواضيع طبية MeSH: | Blood Platelets* , Megakaryocytes*, Cell Proliferation ; Stromal Cells |
| مستخلص: | Bone marrow stromal cells provide a proper environment for the development of hematologic lineages. The incorporation of different stromal cells into in vitro culture systems would be an attractive model to study megakaryopoiesis and thrombopoiesis. Our objective was to evaluate the participation of different types of stromal cells in in vitro megakaryopoiesis, thrombopoiesis, and megakaryocyte (MK) survival. CD34-positive progenitors from umbilical cord blood were differentiated into MK precursors and then cocultured with umbilical vein endothelial cells (HUVECs), bone marrow mesenchymal stem cells (MSCs), skin fibroblasts (SFs) (all human), or the mouse fibroblast cell line L929. The number of MKs (CD61-positive cells) was increased in the presence of HUVECs and SFs, whereas L929 cells decreased total and mature MK counts. With respect to thrombopoiesis, HUVECs increased proplatelet (PP)-producing MKs, while MSCs, L929 cells, and SFs had the opposite effect (immunofluorescence staining and microscopic analysis). MK survival was enhanced in MSC and SF co-cultures, as assessed by evaluation of pyknotic nuclei. However, HUVECs and L929 did not prevent apoptosis of MKs. Reciprocally, the cloning efficiency of MSCs was decreased in the presence of MKs, while the ability of stromal cells (either MSCs or SFs) to produce the extracellular matrix proteins type III collagen, fibronectin, dermatan sulfate, heparan sulfate, and prolyl 4-hydroxylase subunit β was preserved. These data indicate that each stromal cell type performs distinctive functions that differentially modulate MK growth and platelet production and, at the same time, that MKs also modify stromal cell behavior. Overall, our results highlight the relevance of considering the influence of stromal cells in MK research as well as the close interplay of different cell types within the bone marrow milieu. Competing Interests: Conflict of interest disclosure All authors declare no competing interests. |
| تواريخ الأحداث: | Date Created: 20220115 Date Completed: 20220505 Latest Revision: 20220610 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.exphem.2022.01.002 |
| PMID: | 35032592 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1873-2399 |
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| DOI: | 10.1016/j.exphem.2022.01.002 |