دورية أكاديمية
Study of the inhibitory effect of STAT1 on PDCoV infection.
| العنوان: | Study of the inhibitory effect of STAT1 on PDCoV infection. |
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| المؤلفون: | Qu H; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: 18428387343@163.com., Wen Y; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: wenyimin666@163.com., Hu J; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: Hu_Jingfei0127@163.com., Xiao D; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: 15838585575@163.com., Li S; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: lishiqian95@126.com., Zhang L; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: zlw1655468752@qq.com., Liao Y; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: liaoyijie819@qq.com., Chen R; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: 18102341339@163.com., Zhao Y; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: zhaoyujia2015@163.com., Wen Y; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: yueliang5189@163.com., Wu R; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: wurui1977@163.com., Zhao Q; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: zhao.qin@sicau.edu.cn., Du S; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: Senyandu@163.com., Yan Q; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: yanqigui@126.com., Wen X; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: xintian3211@126.com., Cao S; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Sichuan Science-observation Experiment Station of Veterinary Drugs and Veterinary Diagnostic Technology, Ministry of Agriculture, Chengdu, 611130, China; National Animal Experiments Teaching Demonstration Center, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: csanjie@sicau.edu.cn., Huang X; Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Sichuan Science-observation Experiment Station of Veterinary Drugs and Veterinary Diagnostic Technology, Ministry of Agriculture, Chengdu, 611130, China; National Animal Experiments Teaching Demonstration Center, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: rsghb110@126.com. |
| المصدر: | Veterinary microbiology [Vet Microbiol] 2022 Mar; Vol. 266, pp. 109333. Date of Electronic Publication: 2022 Jan 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| مواضيع طبية MeSH: | Coronavirus Infections*/veterinary , Swine Diseases*, Animals ; Interferons ; LLC-PK1 Cells ; Swine ; Virus Internalization |
| مستخلص: | Porcine deltacoronavirus (PDCoV) is an enteropathogen found in many pig producing countries. It can cause acute diarrhea, vomiting, dehydration, and death in newborn piglets, seriously affecting the development of pig breeding industries. To date, our knowledge of the pathogenesis of PDCoV and its interactions with host cell factors remains incomplete. Using Co-IP coupled with LC/MS-MS, we identified 67 proteins that potentially interact with PDCoV in LLC-PK1 cells; five of the identified proteins were chosen for further evaluation (IMMT, STAT1, XPO5, PIK3AP1, and TMPRSS11E). Five LLC-PK1 cell lines, each with one of the genes of interest knocked down, were constructed using CRISPR/cas9. In these knockdown cells lines, only STAT1 KD resulted in a significantly greater virus yield. Knockdown of the remaining four genes resulted, to varying degrees, in a lower virus yield that wild-type LLC-PK1 cells. The absence of STAT1 did not significantly affect the attachment of PDCoV to cells, but did result in increased viral internalization. Additionally, PDCoV infection stimulated expression of interferon stimulated genes (ISGs) downstream of STAT1 (IFIT1, IFIT2, RADS2, ISG15, MX1, and OAS1) while knockdown of STAT1 resulted in a greater than 80 % decrease in the expression of all six ISGs. Our findings show that STAT1 interacts with PDCoV, and plays a negative regulatory role in PDCoV infection. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Gene knockdown; Immunoprecipitation; Inhibition; Interaction; PDCoV; STAT1 |
| المشرفين على المادة: | 9008-11-1 (Interferons) |
| تواريخ الأحداث: | Date Created: 20220116 Date Completed: 20220406 Latest Revision: 20220406 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.vetmic.2022.109333 |
| PMID: | 35033844 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2542 |
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| DOI: | 10.1016/j.vetmic.2022.109333 |