دورية أكاديمية
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Size and macromolecule stabilizer-dependent performance of gold colloids in immuno-PCR.

التفاصيل البيبلوغرافية
العنوان: Size and macromolecule stabilizer-dependent performance of gold colloids in immuno-PCR.
المؤلفون: Tabatabaei MS; Department of Chemistry, University of Prince Edward Island, Prince Edward Island, Charlottetown, C1A 4P3, Canada., Islam R; Somru Bioscience, Charlottetown, PE, Canada., Ahmed M; Department of Chemistry, University of Prince Edward Island, Prince Edward Island, Charlottetown, C1A 4P3, Canada. marahmed@upei.ca.; Faculty of Sustainable Design Engineering, University of Prince Edward Island, Prince Edward Island, Charlottetown, C1A 4P3, Canada. marahmed@upei.ca.
المصدر: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2022 Mar; Vol. 414 (6), pp. 2205-2217. Date of Electronic Publication: 2022 Jan 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Heidelberg : Springer-Verlag, 2002-
مواضيع طبية MeSH: Gold*/chemistry , Metal Nanoparticles*/chemistry, Gold Colloid ; Immunoassay ; Particle Size ; Polymerase Chain Reaction/methods
مستخلص: Gold nanoparticles (GNPs) are well-documented for their size and surface chemistry-dependent electronic and optical properties that are extensively utilized to develop highly sensitive immunoassays. GNP-based immuno-polymerase chain reaction (immuno-PCR) is especially interesting due to the facile loading of biomolecules on the surface of GNP probes and has been utilized to develop analyte-specific assays. In this study, the role of size and surface chemistry of GNPs is explored in detail to develop a highly sensitive and reproducible immuno-PCR assay for specific detection of biotinylated analytes. Our results indicate that smaller-sized gold nanoparticles outperform the larger ones in terms of their sensitivity in immuno-PCR assay and show superior loading of proteins and oligonucleotides on the surface of nanoparticles. Furthermore, the role of different macromolecular stabilizers (such as polyethylene glycol (PEG), bovine serum albumin (BSA), and PEGylated BSA) was compared to optimize the loading of biomolecules and to improve the signal-to-noise ratio of GNP probes. mPEG-BSA-functionalized GNP probes of 15 nm were found to be highly sensitive at low concentrations of analytes and significantly (~ 30 fold) improve the limit of detection of analytes in comparison with ELISA assay.
(© 2022. Springer-Verlag GmbH Germany, part of Springer Nature.)
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فهرسة مساهمة: Keywords: GNP-based immuno-PCR; High sensitivity; Macromolecule stabilizers; Reproducibility; Size-dependent loading of biomolecules
المشرفين على المادة: 0 (Gold Colloid)
7440-57-5 (Gold)
تواريخ الأحداث: Date Created: 20220116 Date Completed: 20220331 Latest Revision: 20220401
رمز التحديث: 20231215
DOI: 10.1007/s00216-021-03857-9
PMID: 35034157
قاعدة البيانات: MEDLINE
الوصف
تدمد:1618-2650
DOI:10.1007/s00216-021-03857-9