دورية أكاديمية
Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein.
العنوان: | Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein. |
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المؤلفون: | Pereira LR; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Alves RPDS; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Sales NS; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Andreata-Santos R; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Venceslau-Carvalho AA; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Pereira SS; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Castro-Amarante MF; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Rodrigues-Jesus MJ; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Favaro MTP; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Chura-Chambi RM; Biotechnology Center, Institute of Energy and Nuclear Research (IPEN), São Paulo, Brazil., Morganti L; Biotechnology Center, Institute of Energy and Nuclear Research (IPEN), São Paulo, Brazil., Ferreira LCS; Laboratory of Vaccine Development, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. |
المصدر: | Frontiers in medical technology [Front Med Technol] 2020 Dec 03; Vol. 2, pp. 604160. Date of Electronic Publication: 2020 Dec 03 (Print Publication: 2020). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Country of Publication: Switzerland NLM ID: 101772626 Publication Model: eCollection Cited Medium: Internet ISSN: 2673-3129 (Electronic) Linking ISSN: 26733129 NLM ISO Abbreviation: Front Med Technol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne, Switzerland : Frontiers, [2019]- |
مستخلص: | Zika virus (ZIKV) is a globally-distributed flavivirus transmitted to humans by Aedes mosquitoes, usually causing mild symptoms that may evolve to severe conditions, including neurological alterations, such as neonatal microcephaly and Guillain-Barré syndrome. Due to the absence of specific and effective preventive methods, we designed a new subunit vaccine based on a DNA vector (pgDNS1-ZIKV) encoding the non-structural protein 1 (NS1) genetically fused to the Herpes Simplex Virus (HSV) glycoprotein D (gD) protein. Recombinant plasmids were replicated in Escherichia coli and the expression of the target protein was confirmed in transfected HEK293 cells. C57BL/6 and AB6 (IFNAR1-/-) mice were i.m. immunized by electroporation in order to evaluate pgDNS1-ZIKV immunogenicity. After two doses, high NS1-specific IgG antibody titers were measured in serum samples collected from pgDNS1-ZIKV-immunized mice. The NS1-specific antibodies were capable to bind the native protein expressed in infected mammalian cells. Immunization with pgDNS1-ZIKV increased both humoral and cellular immune responses regarding mice immunized with a ZIKV NS1 encoding vaccine. Immunization with pgDNS1-ZIKV reduced viremia and morbidity scores leading to enhanced survival of immunodeficient AB6 mice challenged with a lethal virus load. These results give support to the use of ZIKV NS1 as a target antigen and further demonstrate the relevant adjuvant effects of HSV-1 gD. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2020 Pereira, Alves, Sales, Andreata-Santos, Venceslau-Carvalho, Pereira, Castro-Amarante, Rodrigues-Jesus, Favaro, Chura-Chambi, Morganti and Ferreira.) |
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فهرسة مساهمة: | Keywords: DNA vaccine; HSV-1; NS1 protein; Zika virus; flavivirus; gD protein |
تواريخ الأحداث: | Date Created: 20220120 Latest Revision: 20221229 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC8757838 |
DOI: | 10.3389/fmedt.2020.604160 |
PMID: | 35047887 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2673-3129 |
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DOI: | 10.3389/fmedt.2020.604160 |