دورية أكاديمية
أعرض في EDS

Molecular Characterization and Functional Analysis of the Nattectin-like Toxin from the Venomous Fish Thalassophryne maculosa .

التفاصيل البيبلوغرافية
العنوان: Molecular Characterization and Functional Analysis of the Nattectin-like Toxin from the Venomous Fish Thalassophryne maculosa .
المؤلفون: Lopes-Ferreira M; Immunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP), Butantan Institute, Vital Brasil Avenue, 1500 Butantan, Sao Paulo 05503-009, Brazil., Sosa-Rosales I; Escuela de Ciências Aplicadas del Mar, Universidad de Oriente, Boca de Rio 6304, Venezuela., Silva Junior PI; Protein Chemistry Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP), Butantan Institute, Vital Brasil Avenue, 1500 Butantan, Sao Paulo 05503-009, Brazil., Conceicao K; Peptide Biochemistry Laboratory, UNIFESP, Sao Jose dos Campos 12247-014, Brazil., Maleski ALA; Immunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP), Butantan Institute, Vital Brasil Avenue, 1500 Butantan, Sao Paulo 05503-009, Brazil.; Post-Graduation Program of Toxinology, Butantan Institute, Vital Brasil Avenue, 1500 Butantan, Sao Paulo 05503-009, Brazil., Balan-Lima L; Immunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP), Butantan Institute, Vital Brasil Avenue, 1500 Butantan, Sao Paulo 05503-009, Brazil., Disner GR; Immunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP), Butantan Institute, Vital Brasil Avenue, 1500 Butantan, Sao Paulo 05503-009, Brazil., Lima C; Immunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP), Butantan Institute, Vital Brasil Avenue, 1500 Butantan, Sao Paulo 05503-009, Brazil.
المصدر: Toxins [Toxins (Basel)] 2021 Dec 21; Vol. 14 (1). Date of Electronic Publication: 2021 Dec 21.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Batrachoidiformes*, Fish Venoms/*chemistry , Lectins, C-Type/*chemistry , Marine Toxins/*chemistry, Amino Acid Sequence ; Animals ; Fish Venoms/pharmacology ; Marine Toxins/pharmacology
مستخلص: TmC4-47.2 is a toxin with myotoxic activity found in the venom of Thalassophryne maculosa , a venomous fish commonly found in Latin America whose envenomation produces an injury characterized by delayed neutrophil migration, production of major pro-inflammatory cytokines, and necrosis at the wound site, as well as a specific systemic immune response. However, there are few studies on the protein structure and functions associated with it. Here, the toxin was identified from the crude venom by chromatography and protein purification systems. TmC4-47.2 shows high homology with the Nattectin from Thalassophryne nattereri venom, with 6 cysteines and QPD domain for binding to galactose. We confirm its hemagglutinating and microbicide abilities independent of carbohydrate binding, supporting its classification as a nattectin-like lectin. After performing the characterization of TmC4-47.2, we verified its ability to induce an increase in the rolling and adherence of leukocytes in cremaster post-capillary venules dependent on the α5β1 integrin. Finally, we could observe the inflammatory activity of TmC4-47.2 through the production of IL-6 and eotaxin in the peritoneal cavity with sustained recruitment of eosinophils and neutrophils up to 24 h. Together, our study characterized a nattectin-like protein from T. maculosa , pointing to its role as a molecule involved in the carbohydrate-independent agglutination response and modulation of eosinophilic and neutrophilic inflammation.
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فهرسة مساهمة: Keywords: MALDI-ToF; Thalassophryne; animal toxins; antibacterial activity; hemagglutinating activity; inflammation; nattectin; reverse-phase HPLC; toxinology
المشرفين على المادة: 0 (Fish Venoms)
0 (Lectins, C-Type)
0 (Marine Toxins)
تواريخ الأحداث: Date Created: 20220120 Date Completed: 20220301 Latest Revision: 20220301
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8778695
DOI: 10.3390/toxins14010002
PMID: 35050979
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6651
DOI:10.3390/toxins14010002