دورية أكاديمية
أعرض في EDS

Ancient DNA Methods Improve Forensic DNA Profiling of Korean War and World War II Unknowns.

التفاصيل البيبلوغرافية
العنوان: Ancient DNA Methods Improve Forensic DNA Profiling of Korean War and World War II Unknowns.
المؤلفون: Zavala EI; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany., Thomas JT; Armed Forces Medical Examiner System's Armed Forces DNA Identification Laboratory (AFMES-AFDIL), Dover Air Force Base, Dover, DE 19902, USA.; SNA International, Contractor Supporting the Armed Forces Medical Examiner System, Alexandria, VA 22314, USA., Sturk-Andreaggi K; Armed Forces Medical Examiner System's Armed Forces DNA Identification Laboratory (AFMES-AFDIL), Dover Air Force Base, Dover, DE 19902, USA.; SNA International, Contractor Supporting the Armed Forces Medical Examiner System, Alexandria, VA 22314, USA.; Department of Immunology, Genetics and Pathology, Uppsala University, SE-751 08 Uppsala, Sweden., Daniels-Higginbotham J; Armed Forces Medical Examiner System's Armed Forces DNA Identification Laboratory (AFMES-AFDIL), Dover Air Force Base, Dover, DE 19902, USA.; SNA International, Contractor Supporting the Armed Forces Medical Examiner System, Alexandria, VA 22314, USA., Meyers KK; Armed Forces Medical Examiner System's Armed Forces DNA Identification Laboratory (AFMES-AFDIL), Dover Air Force Base, Dover, DE 19902, USA.; SNA International, Contractor Supporting the Armed Forces Medical Examiner System, Alexandria, VA 22314, USA., Barrit-Ross S; Armed Forces Medical Examiner System's Armed Forces DNA Identification Laboratory (AFMES-AFDIL), Dover Air Force Base, Dover, DE 19902, USA., Aximu-Petri A; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany., Richter J; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany., Nickel B; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany., Berg GE; Defense Personnel Accounting Agency, Central Identification Laboratory, Hickam Air Force Base, Oahu, HI 96853, USA., McMahon TP; Armed Forces Medical Examiner System's Armed Forces DNA Identification Laboratory (AFMES-AFDIL), Dover Air Force Base, Dover, DE 19902, USA.; SNA International, Contractor Supporting the Armed Forces Medical Examiner System, Alexandria, VA 22314, USA., Meyer M; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany., Marshall C; Armed Forces Medical Examiner System's Armed Forces DNA Identification Laboratory (AFMES-AFDIL), Dover Air Force Base, Dover, DE 19902, USA.; SNA International, Contractor Supporting the Armed Forces Medical Examiner System, Alexandria, VA 22314, USA.; Forensic Science Program, Pennsylvania State University, State College, PA 16802, USA.
المصدر: Genes [Genes (Basel)] 2022 Jan 11; Vol. 13 (1). Date of Electronic Publication: 2022 Jan 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Forensic Genetics* , Polymorphism, Single Nucleotide*, Body Remains/*metabolism , DNA Fingerprinting/*methods , DNA, Ancient/*analysis , High-Throughput Nucleotide Sequencing/*methods , Sequence Analysis, DNA/*methods, DNA, Ancient/isolation & purification ; Humans ; Korean War ; World War II
مستخلص: The integration of massively parallel sequencing (MPS) technology into forensic casework has been of particular benefit to the identification of unknown military service members. However, highly degraded or chemically treated skeletal remains often fail to provide usable DNA profiles, even with sensitive mitochondrial (mt) DNA capture and MPS methods. In parallel, the ancient DNA field has developed workflows specifically for degraded DNA, resulting in the successful recovery of nuclear DNA and mtDNA from skeletal remains as well as sediment over 100,000 years old. In this study we use a set of disinterred skeletal remains from the Korean War and World War II to test if ancient DNA extraction and library preparation methods improve forensic DNA profiling. We identified an ancient DNA extraction protocol that resulted in the recovery of significantly more human mtDNA fragments than protocols previously used in casework. In addition, utilizing single-stranded rather than double-stranded library preparation resulted in increased attainment of reportable mtDNA profiles. This study emphasizes that the combination of ancient DNA extraction and library preparation methods evaluated here increases the success rate of DNA profiling, and likelihood of identifying historical remains.
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فهرسة مساهمة: Keywords: ancient DNA; degraded DNA; forensic DNA profiling; human identification; massively parallel sequencing (MPS); mitochondrial DNA
المشرفين على المادة: 0 (DNA, Ancient)
تواريخ الأحداث: Date Created: 20220121 Date Completed: 20220222 Latest Revision: 20220222
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8774965
DOI: 10.3390/genes13010129
PMID: 35052469
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4425
DOI:10.3390/genes13010129