دورية أكاديمية
أعرض في EDS

Towards Biohybrid Lung Development-Fibronectin-Coating Bestows Hemocompatibility of Gas Exchange Hollow Fiber Membranes by Improving Flow-Resistant Endothelialization.

التفاصيل البيبلوغرافية
العنوان: Towards Biohybrid Lung Development-Fibronectin-Coating Bestows Hemocompatibility of Gas Exchange Hollow Fiber Membranes by Improving Flow-Resistant Endothelialization.
المؤلفون: Pflaum M; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany., Jurmann S; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany., Katsirntaki K; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany., Mälzer M; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany., Haverich A; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany.; BREATH (Biomedical Research in Endstage and Obstructive Lung Disease Hannover), German Center for Lung Research (DZL), Carl-Neuberg Str. 1, 30625 Hannover, Germany., Wiegmann B; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany.; BREATH (Biomedical Research in Endstage and Obstructive Lung Disease Hannover), German Center for Lung Research (DZL), Carl-Neuberg Str. 1, 30625 Hannover, Germany.
المصدر: Membranes [Membranes (Basel)] 2021 Dec 27; Vol. 12 (1). Date of Electronic Publication: 2021 Dec 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101577807 Publication Model: Electronic Cited Medium: Print ISSN: 2077-0375 (Print) Linking ISSN: 20770375 NLM ISO Abbreviation: Membranes (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Publishing
مستخلص: To provide an alternative treatment option for patients with end-stage lung disease, we aim for biohybrid lung development (BHL) based on hollow fiber membrane (HFM) technology used in extracorporeal membrane oxygenators. For long-term BHL application, complete hemocompatibility of all blood-contacting surfaces is indispensable and can be achieved by their endothelialization. Indeed, albumin/heparin (AH) coated HFM enables initial endothelialization, but as inexplicable cell loss under flow conditions was seen, we assessed an alternative HFM coating using fibronectin (FN). Therefore, endothelial cell (EC) adherence and viability on both coated HFM were analyzed by fluorescence-based staining. Functional leukocyte and thrombocyte adhesion assays were performed to evaluate hemocompatibility, also in comparison to blood plasma coated HFM as a clinically relevant control. To assess monolayer resistance and EC behavior under clinically relevant flow conditions, a mock circulation setup was established, which also facilitates imitation of lung-disease specific blood gas settings. Besides quantification of flow-associated cell loss, endothelial responses towards external stimuli, like flow exposure or TNFα stimulation, were analyzed by qRT-PCR, focusing on inflammation, thrombus formation and extracellular matrix production. Under static conditions, both coated HFM enabled the generation of a viable, confluent, non-inflammatory and anti-thrombogenic monolayer. However, by means of homogenous FN coating, cell retention and physiologic gene regulation towards an improved hemocompatible-and extracellular matrix producing phenotype, was significantly superior compared to the inhomogeneous AH coating. In summary, our adaptable in-house FN coating secures the endothelial requirements for long-term BHL application and may promote monolayer establishment on all other blood contacting surfaces of the BHL (e.g., cannulae).
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معلومات مُعتمدة: 347346497 Deutsche Forschungsgemeinschaft; 82DZL00201 German Center for Lung Research
فهرسة مساهمة: Keywords: biohybrid lung; endothelialization; hemocompatible surface
تواريخ الأحداث: Date Created: 20220121 Latest Revision: 20220128
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8779364
DOI: 10.3390/membranes12010035
PMID: 35054561
قاعدة البيانات: MEDLINE
الوصف
تدمد:2077-0375
DOI:10.3390/membranes12010035