دورية أكاديمية
Towards Biohybrid Lung Development-Fibronectin-Coating Bestows Hemocompatibility of Gas Exchange Hollow Fiber Membranes by Improving Flow-Resistant Endothelialization.
العنوان: | Towards Biohybrid Lung Development-Fibronectin-Coating Bestows Hemocompatibility of Gas Exchange Hollow Fiber Membranes by Improving Flow-Resistant Endothelialization. |
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المؤلفون: | Pflaum M; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany., Jurmann S; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany., Katsirntaki K; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany., Mälzer M; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany., Haverich A; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany.; BREATH (Biomedical Research in Endstage and Obstructive Lung Disease Hannover), German Center for Lung Research (DZL), Carl-Neuberg Str. 1, 30625 Hannover, Germany., Wiegmann B; Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.; Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany.; BREATH (Biomedical Research in Endstage and Obstructive Lung Disease Hannover), German Center for Lung Research (DZL), Carl-Neuberg Str. 1, 30625 Hannover, Germany. |
المصدر: | Membranes [Membranes (Basel)] 2021 Dec 27; Vol. 12 (1). Date of Electronic Publication: 2021 Dec 27. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101577807 Publication Model: Electronic Cited Medium: Print ISSN: 2077-0375 (Print) Linking ISSN: 20770375 NLM ISO Abbreviation: Membranes (Basel) Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI Publishing |
مستخلص: | To provide an alternative treatment option for patients with end-stage lung disease, we aim for biohybrid lung development (BHL) based on hollow fiber membrane (HFM) technology used in extracorporeal membrane oxygenators. For long-term BHL application, complete hemocompatibility of all blood-contacting surfaces is indispensable and can be achieved by their endothelialization. Indeed, albumin/heparin (AH) coated HFM enables initial endothelialization, but as inexplicable cell loss under flow conditions was seen, we assessed an alternative HFM coating using fibronectin (FN). Therefore, endothelial cell (EC) adherence and viability on both coated HFM were analyzed by fluorescence-based staining. Functional leukocyte and thrombocyte adhesion assays were performed to evaluate hemocompatibility, also in comparison to blood plasma coated HFM as a clinically relevant control. To assess monolayer resistance and EC behavior under clinically relevant flow conditions, a mock circulation setup was established, which also facilitates imitation of lung-disease specific blood gas settings. Besides quantification of flow-associated cell loss, endothelial responses towards external stimuli, like flow exposure or TNFα stimulation, were analyzed by qRT-PCR, focusing on inflammation, thrombus formation and extracellular matrix production. Under static conditions, both coated HFM enabled the generation of a viable, confluent, non-inflammatory and anti-thrombogenic monolayer. However, by means of homogenous FN coating, cell retention and physiologic gene regulation towards an improved hemocompatible-and extracellular matrix producing phenotype, was significantly superior compared to the inhomogeneous AH coating. In summary, our adaptable in-house FN coating secures the endothelial requirements for long-term BHL application and may promote monolayer establishment on all other blood contacting surfaces of the BHL (e.g., cannulae). |
References: | J Cell Mol Med. 2017 Aug;21(8):1457-1462. (PMID: 28211170) Arterioscler Thromb Vasc Biol. 2020 Feb;40(2):350-364. (PMID: 31826652) Blood. 2002 Sep 1;100(5):1689-98. (PMID: 12176889) Cytotechnology. 2010 Jan;62(1):17-22. (PMID: 20217226) Histol Histopathol. 2002 Oct;17(4):1163-71. (PMID: 12371144) Biomaterials. 2014 Sep;35(28):8123-33. (PMID: 24961166) Sci Transl Med. 2018 Aug 1;10(452):. (PMID: 30068570) Microvasc Res. 2021 Mar;134:104107. (PMID: 33212112) Clin Res Cardiol. 2016 Apr;105(4):283-96. (PMID: 26608160) Tissue Eng Part A. 2010 Oct;16(10):3043-53. (PMID: 20486793) Int J Mol Sci. 2021 Feb 22;22(4):. (PMID: 33671524) J Tissue Eng Regen Med. 2018 Dec;12(12):2319-2330. (PMID: 30362254) Annu Rev Cell Dev Biol. 1996;12:697-715. (PMID: 8970741) Ann Thorac Surg. 2017 Aug;104(2):412-419. (PMID: 28242078) Cells. 2020 Mar 23;9(3):. (PMID: 32210018) J Am Coll Cardiol. 2005 May 17;45(10):1574-9. (PMID: 15893169) Xenotransplantation. 2011 Mar-Apr;18(2):94-107. (PMID: 21496117) Front Physiol. 2018 Dec 04;9:1739. (PMID: 30564143) Biotechnol Bioeng. 2012 Jul;109(7):1808-16. (PMID: 22359201) Arterioscler Thromb Vasc Biol. 2011 Aug;31(8):1908-15. (PMID: 21474821) Eur J Heart Fail. 2017 May;19(5):595-602. (PMID: 28198133) Beilstein J Org Chem. 2013;9:270-7. (PMID: 23504394) Biomaterials. 2007 Jun;28(16):2547-71. (PMID: 17316788) PLoS One. 2015 Jun 19;10(6):e0130749. (PMID: 26090873) Science. 2019 May 3;364(6439):458-464. (PMID: 31048486) Blood Coagul Fibrinolysis. 2012 Jan;23(1):1-10. (PMID: 22036808) J Heart Lung Transplant. 2021 Nov;40(11):1349-1379. (PMID: 34419372) Biomed Eng Online. 2013 Jan 28;12:7. (PMID: 23356939) Blood. 2006 Nov 1;108(9):3035-44. (PMID: 16835373) J Mech Behav Biomed Mater. 2016 Jul;60:301-311. (PMID: 26919566) Circ Res. 2005 Nov 25;97(11):1093-107. (PMID: 16306453) J Biomed Mater Res A. 2014 Jun;102(6):1909-20. (PMID: 23852964) Biochem Biophys Res Commun. 2007 Mar 30;355(1):228-33. (PMID: 17291452) Cell Tissue Res. 2010 Jun;340(3):471-9. (PMID: 20490867) Adv Compos Hybrid Mater. 2021 May 3;:1-18. (PMID: 33969267) Thorac Cardiovasc Surg. 2005 Apr;53(2):96-102. (PMID: 15786008) Acta Biomater. 2017 Mar 1;50:510-521. (PMID: 27956361) Adv Drug Deliv Rev. 2017 Mar;112:12-23. (PMID: 28042080) Circ Res. 2008 Apr 11;102(7):770-6. (PMID: 18258858) J Artif Organs. 2018 Mar;21(1):68-75. (PMID: 28879605) Tissue Eng Part C Methods. 2012 Sep;18(9):688-96. (PMID: 22429453) Micromachines (Basel). 2021 Aug 19;12(8):. (PMID: 34442603) Annu Rev Cell Dev Biol. 1996;12:463-518. (PMID: 8970735) Ann Am Thorac Soc. 2015 Mar;12 Suppl 1:S45-9. (PMID: 25830835) Semin Thromb Hemost. 2018 Feb;44(1):20-29. (PMID: 28898902) J Vasc Surg. 1990 Aug;12(2):126-30. (PMID: 2381027) Ann N Y Acad Sci. 1978 Jun 20;312:151-9. (PMID: 291359) Lab Chip. 2018 Dec 4;18(24):3780-3789. (PMID: 30421770) Nature. 1984 May 3-9;309(5963):30-3. (PMID: 6325925) Biomaterials. 2004 Nov;25(26):5681-703. (PMID: 15147815) Int J Artif Organs. 2005 Jan;28(1):30-4. (PMID: 15742307) PLoS One. 2014 Dec 02;9(12):e112316. (PMID: 25464516) |
معلومات مُعتمدة: | 347346497 Deutsche Forschungsgemeinschaft; 82DZL00201 German Center for Lung Research |
فهرسة مساهمة: | Keywords: biohybrid lung; endothelialization; hemocompatible surface |
تواريخ الأحداث: | Date Created: 20220121 Latest Revision: 20220128 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC8779364 |
DOI: | 10.3390/membranes12010035 |
PMID: | 35054561 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2077-0375 |
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DOI: | 10.3390/membranes12010035 |