دورية أكاديمية
أعرض في EDS

Interactive Dynamics of Cell Volume and Cell Death in Human Erythrocytes Exposed to α-Hemolysin from Escherichia coli .

التفاصيل البيبلوغرافية
العنوان: Interactive Dynamics of Cell Volume and Cell Death in Human Erythrocytes Exposed to α-Hemolysin from Escherichia coli .
المؤلفون: Saffioti NA; Laboratorio de Biosensores Avanzados, Instituto de Nanosistemas, Universidad Nacional de San Martín, San Martín, Buenos Aires 1650, Argentina.; Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, Argentina., Lauri N; Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, Argentina.; Instituto de Química y Físico-Química Biológicas 'Prof. Alejandro C. Paladini', UBA, CONICET, Facultad de Farmacia y Bioquímica, Junín 956, Buenos Aires 1113, Argentina., Cané L; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP) CCT-La Plata, CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 60 y 120, La Plata, Buenos Aires 1900, Argentina., Gonzalez-Lebrero R; Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, Argentina.; Instituto de Química y Físico-Química Biológicas 'Prof. Alejandro C. Paladini', UBA, CONICET, Facultad de Farmacia y Bioquímica, Junín 956, Buenos Aires 1113, Argentina., Alleva K; Instituto de Química y Físico-Química Biológicas 'Prof. Alejandro C. Paladini', UBA, CONICET, Facultad de Farmacia y Bioquímica, Junín 956, Buenos Aires 1113, Argentina., Mouro-Chanteloup I; Université de Paris and Université des Antilles, INSERM, BIGR, F-75015 Paris, France., Ostuni MA; Université de Paris and Université des Antilles, INSERM, BIGR, F-75015 Paris, France., Herlax V; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP) CCT-La Plata, CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 60 y 120, La Plata, Buenos Aires 1900, Argentina., Schwarzbaum PJ; Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires 1113, Argentina.; Instituto de Química y Físico-Química Biológicas 'Prof. Alejandro C. Paladini', UBA, CONICET, Facultad de Farmacia y Bioquímica, Junín 956, Buenos Aires 1113, Argentina.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2022 Jan 14; Vol. 23 (2). Date of Electronic Publication: 2022 Jan 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Cell Size* , Models, Biological*, Erythrocytes/*cytology , Erythrocytes/*physiology , Escherichia coli Proteins/*pharmacology , Hemolysin Proteins/*pharmacology, Adenosine Triphosphate/metabolism ; Biomarkers ; Cell Death/drug effects ; Cell Death/immunology ; Dose-Response Relationship, Drug ; Escherichia coli Proteins/immunology ; Hemolysin Proteins/immunology ; Hemolysis ; Host-Pathogen Interactions ; Humans ; Kinetics ; Permeability
مستخلص: α-hemolysin (HlyA) of E. coli binds irreversibly to human erythrocytes and induces cell swelling, ultimately leading to hemolysis. We characterized the mechanism involved in water transport induced by HlyA and analyzed how swelling and hemolysis might be coupled. Osmotic water permeability (P f ) was assessed by stopped-flow light scattering. Preincubation with HlyA strongly reduced P f in control- and aquaporin 1-null red blood cells, although the relative P f decrease was similar in both cell types. The dynamics of cell volume and hemolysis on RBCs was assessed by electrical impedance, light dispersion and hemoglobin release. Results show that HlyA induced erythrocyte swelling, which is enhanced by purinergic signaling, and is coupled to osmotic hemolysis. We propose a mathematical model of HlyA activity where the kinetics of cell volume and hemolysis in human erythrocytes depend on the flux of osmolytes across the membrane, and on the maximum volume that these cells can tolerate. Our results provide new insights for understanding signaling and cytotoxicity mediated by HlyA in erythrocytes.
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معلومات مُعتمدة: 200201701001 52BA University of Buenos Aires; PICT 2019-03218 Agencia Nacional de Promoción Científica y Tecnológica; A15S01 Comité ECOS Sud; Guest Researcher Programm Université de Paris
فهرسة مساهمة: Keywords: RTX toxins; aquaporins; cell volume regulation; purinergic
المشرفين على المادة: 0 (Biomarkers)
0 (Escherichia coli Proteins)
0 (Hemolysin Proteins)
8L70Q75FXE (Adenosine Triphosphate)
تواريخ الأحداث: Date Created: 20220121 Date Completed: 20220203 Latest Revision: 20220203
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8778525
DOI: 10.3390/ijms23020872
PMID: 35055067
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms23020872