دورية أكاديمية
When Mosquito HV bites Biomark HD: An automated workflow for high-throughput qPCR.
| العنوان: | When Mosquito HV bites Biomark HD: An automated workflow for high-throughput qPCR. |
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| المؤلفون: | Manjunath HS; Integrated Genomic Services, Research Department, Sidra Medicine, Doha, Qatar., Kalikiri MKR; Integrated Genomic Services, Research Department, Sidra Medicine, Doha, Qatar., Kabeer BSA; System Biology, Research Department, Sidra Medicine, Doha, Qatar., Tomei S; Integrated Genomic Services, Research Department, Sidra Medicine, Doha, Qatar. Electronic address: stomei@sidra.org. |
| المصدر: | SLAS technology [SLAS Technol] 2022 Jun; Vol. 27 (3), pp. 219-223. Date of Electronic Publication: 2022 Jan 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: SAGE Publications Country of Publication: United States NLM ID: 101697564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2472-6311 (Electronic) Linking ISSN: 24726303 NLM ISO Abbreviation: SLAS Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Thousand Oaks, CA : SAGE Publications, [2017]- |
| مواضيع طبية MeSH: | COVID-19*/diagnosis , Culicidae*, Animals ; COVID-19 Testing ; Humans ; SARS-CoV-2/genetics ; Workflow |
| مستخلص: | Automation solutions can significantly improve sample processing efficiency and can be of particular help in core facility settings. Centralized core facilities are being established world-wide aimed at strengthening institutions' clinical and research enterprises and at addressing the need to process large volumes of samples on expensive cutting-edge technologies in a limited time. High-throughput qPCR profiling is a service offered by most genomics facilities. Several platforms have been developed to process large numbers of samples in a short time, including the Fluidigm Biomark HD, which has also been proved useful to increase the SARS-CoV-2 testing capacities. Several automation systems are currently available to miniaturize volumes and improve bioanalytical workflows, including the SPT Labtech Mosquito HV system. Here we have applied the Mosquito HV platform for the automation of the sample preparation of the Fluidigm gene expression workflow. We have successfully automated the pre-amplification and exonuclease cleanup steps with the aim of reducing manual error and sample processing time. We show consistency in the expression of reference genes when assessing pooled RNA control samples for the manual and automated workflows of Fluidigm gene expression profiling. Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest or relationships relevant to the content of this paper. All authors take responsibility for all aspects of the reliability of the data presented and their discussed interpretation. (Copyright © 2022. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Automation; Core Facility; Fluidigm; Mosquito HV; RNA; Transcriptomics; high-throughput |
| تواريخ الأحداث: | Date Created: 20220121 Date Completed: 20220609 Latest Revision: 20220624 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.slast.2021.12.007 |
| PMID: | 35058195 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2472-6311 |
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| DOI: | 10.1016/j.slast.2021.12.007 |