دورية أكاديمية
أعرض في EDS

Timothy Grass Pollen Induces Spatial Reorganisation of F-Actin and Loss of Junctional Integrity in Respiratory Cells.

التفاصيل البيبلوغرافية
العنوان: Timothy Grass Pollen Induces Spatial Reorganisation of F-Actin and Loss of Junctional Integrity in Respiratory Cells.
المؤلفون: Bradbury P; Respiratory Technology, Woolcock Institute of Medical Research, Sydney, Australia., Cidem A; Respiratory Technology, Woolcock Institute of Medical Research, Sydney, Australia., Mahmodi H; School of Mathematical and Physical Sciences, University of Technology Sydney, Sydney, Australia., Davies JM; School of Biomedical Science, Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane, Australia., Spicer PT; School of Chemical Engineering, UNSW, Sydney, Australia., Prescott SW; School of Chemical Engineering, UNSW, Sydney, Australia., Kabakova I; School of Mathematical and Physical Sciences, University of Technology Sydney, Sydney, Australia., Ong HX; Respiratory Technology, Woolcock Institute of Medical Research, Sydney, Australia. huixin.ong@mq.edu.au.; Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, Australia. huixin.ong@mq.edu.au., Traini D; Respiratory Technology, Woolcock Institute of Medical Research, Sydney, Australia. daniela.traini@mq.edu.au.; Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, Australia. daniela.traini@mq.edu.au.
المصدر: Inflammation [Inflammation] 2022 Jun; Vol. 45 (3), pp. 1209-1223. Date of Electronic Publication: 2022 Jan 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 7600105 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-2576 (Electronic) Linking ISSN: 03603997 NLM ISO Abbreviation: Inflammation Subsets: MEDLINE
أسماء مطبوعة: Publication: 1999- : New York, NY : Kluwer Academic/Plenum Publishers
Original Publication: New York, Plenum Press.
مواضيع طبية MeSH: Asthma* , Phleum*, Actin Cytoskeleton ; Actins ; Allergens ; Cadherins ; Humans ; Poaceae ; Pollen
مستخلص: Grass pollens have been identified as mediators of respiratory distress, capable of exacerbating respiratory diseases including epidemic thunderstorm asthma (ETSA). It is hypothesised that during thunderstorms, grass pollen grains swell to absorb atmospheric water, rupture, and release internal protein content to the atmosphere. The inhalation of atmospheric grass pollen proteins results in deadly ETSA events. We sought to identify the underlying cellular mechanisms that may contribute towards the severity of ETSA in temperate climates using Timothy grass (Phleum pratense). Respiratory cells exposed to Timothy grass pollen protein extract (PPE) caused cells to undergo hypoxia ultimately triggering the subcellular re-organisation of F-actin from the peri junctional belt to cytoplasmic fibre assembly traversing the cell body. This change in actin configuration coincided with the spatial reorganisation of microtubules and importantly, decreased cell compressibility specifically at the cell centre. Further to this, we find that the pollen-induced reorganisation of the actin cytoskeleton prompting secretion of the pro-inflammatory cytokine, interleukin-8. In addition, the loss of peri-junctional actin following exposure to pollen proteins was accompanied by the release of epithelial transmembrane protein, E-cadherin from cell-cell junctions resulting in a decrease in epithelial barrier integrity. We demonstrate that Timothy grass pollen regulates F-actin dynamics and E-cadherin localisation in respiratory cells to mediate cell-cell junctional integrity highlighting a possible molecular pathway underpinning ETSA events.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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معلومات مُعتمدة: DP190100376 Australian Research Council
فهرسة مساهمة: Keywords: Actin dynamics; Brillouin microscopy; E-cadherin; Hypoxia; Membrane integrity; Thunderstorm asthma; Timothy grass pollen
المشرفين على المادة: 0 (Actins)
0 (Allergens)
0 (Cadherins)
تواريخ الأحداث: Date Created: 20220129 Date Completed: 20220516 Latest Revision: 20220516
رمز التحديث: 20221213
DOI: 10.1007/s10753-021-01614-9
PMID: 35091893
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-2576
DOI:10.1007/s10753-021-01614-9