دورية أكاديمية
Essential role of extracytoplasmic proteins in the resistance of Gluconacetobacter diazotrophicus to cadmium.
العنوان: | Essential role of extracytoplasmic proteins in the resistance of Gluconacetobacter diazotrophicus to cadmium. |
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المؤلفون: | Moreira JR; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: juliarosa_93@hotmail.com., Leandro MR; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: marianaramosl@hotmail.com., Vespoli LS; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: lucianovespoli@gmail.com., Andrade LF; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: leandrofean@hotmail.com., Pimentel VR; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: vivian.rpimentel@gmail.com., Soares FS; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: soares_fabiano@hotmail.com., de Souza SA; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: suzaneariadina@hotmail.com., Intorne AC; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: aline.intorne@ifrj.edu.br., Silveira V; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: vanildo@uenf.br., de Souza Filho GA; Laboratório de Biotecnologia (Setor de Biologia Integrativa), Universidade Estadual do Norte, Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: goncalos@uef.br. |
المصدر: | Research in microbiology [Res Microbiol] 2022 May-Jun; Vol. 173 (4-5), pp. 103922. Date of Electronic Publication: 2022 Jan 31. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: France NLM ID: 8907468 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1769-7123 (Electronic) Linking ISSN: 09232508 NLM ISO Abbreviation: Res Microbiol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Paris : Elsevier Original Publication: Amsterdam ; New York : Elsevier, [c1989- |
مواضيع طبية MeSH: | Cadmium*/metabolism , Gluconacetobacter*/genetics , Gluconacetobacter*/metabolism, Plants/microbiology ; Proteomics |
مستخلص: | Cadmium (Cd) is a heavy metal used as raw material for several fertilizers and pesticides. The increase of Cd concentration in soils has been observed in cultivated areas, affecting animals, plants, and microorganisms. Gluconacetobacter diazotrophicus is a plant growth-promoting bacterium able to survive under adverse environmental conditions. Here, we investigated key mechanisms involved with the resistance of G. diazotrophicus to Cd. Proteomic analyses revealed that the main pathways regulated in response to Cd are nutrient uptake, multidrug efflux pumps, response to oxidative stress, and protein quality control system. Extracytoplasmic proteins related to multidrug efflux pumps were up-accumulated, while several proteins related to nutrients uptake were down-accumulated. The relevance of these pathways for bacterial resistance to Cd was investigated by reverse genetic analysis using mutants defective for nutrient uptake (tdbr, ompW, and oprB), multidrug efflux (czcC), response to oxidative stress (ggt), and protein quality control system (clpX). Our data demonstrated the essential role of the tdbr and czcC genes for resistance to Cd in G. diazotrophicus. These results contribute to a better understanding of the resistance mechanisms to Cd in G. diazotrophicus, shedding light on responses associated with extracytoplasmic compartments. Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest. (Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.) |
فهرسة مساهمة: | Keywords: Efflux pump; Heavy metal; Mutagenesis; Nutrients uptake; Plant growth-promoting bacteria; Proteome |
المشرفين على المادة: | 00BH33GNGH (Cadmium) |
SCR Organism: | Gluconacetobacter diazotrophicus |
تواريخ الأحداث: | Date Created: 20220201 Date Completed: 20220524 Latest Revision: 20220524 |
رمز التحديث: | 20231215 |
DOI: | 10.1016/j.resmic.2022.103922 |
PMID: | 35104604 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1769-7123 |
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DOI: | 10.1016/j.resmic.2022.103922 |