دورية أكاديمية
Deoxyribonucleotide biosynthesis in yeast: assay and properties of ribonucleotide reductase in permeabilized Saccharomyces cerevisiae cells.
| العنوان: | Deoxyribonucleotide biosynthesis in yeast: assay and properties of ribonucleotide reductase in permeabilized Saccharomyces cerevisiae cells. |
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| المؤلفون: | Lammers M, Follmann H |
| المصدر: | Archives of biochemistry and biophysics [Arch Biochem Biophys] 1986 Feb 01; Vol. 244 (2), pp. 430-8. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372430 Publication Model: Print Cited Medium: Print ISSN: 0003-9861 (Print) Linking ISSN: 00039861 NLM ISO Abbreviation: Arch Biochem Biophys Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : San Diego, CA : Elsevier Original Publication: New York, NY : Academic Press |
| مواضيع طبية MeSH: | Deoxyribonucleotides/*biosynthesis , Ribonucleotide Reductases/*analysis , Saccharomyces cerevisiae/*enzymology, Cell Membrane Permeability ; DNA Replication ; DNA, Fungal/analysis ; Genes, Fungal ; Hydroxyurea/pharmacology ; Kinetics ; Mutation ; Phenotype ; Ribonucleotide Reductases/antagonists & inhibitors ; Saccharomyces cerevisiae/drug effects ; Saccharomyces cerevisiae/genetics |
| مستخلص: | Yeast cells permeabilized by freeze-thaw cycles in a sorbitol-containing medium provide an experimentally favorable system for the study of ribonucleotide reduction in a small number of cells or in mutant strains. Ribonucleotide reductase activities determined in such cells are about twice those found in cell extracts but properties of the enzyme, except pH optimum, are closely comparable in both assay procedures. In contrast with other organisms, the activities measured in permeabilized cells from both diploid or haploid strains exceed the demand for deoxyribonucleotide formation during replication of the yeast genome. The method has been applied to yeast cultures growing in the presence of the ribonucleotide reductase inhibitor hydroxyurea and a twofold increase of enzyme activity has been established in such cells. On the other hand, analysis of a series of hus mutants, selected for hydroxyurea sensitivity in the laboratory of Singer and Johnston did not reveal obvious alterations of the enzyme vs the parental strains, suggesting that the hus phenotype may be due to lesions other than in ribonucleotide reductase. |
| المشرفين على المادة: | 0 (DNA, Fungal) 0 (Deoxyribonucleotides) EC 1.17.4.- (Ribonucleotide Reductases) X6Q56QN5QC (Hydroxyurea) |
| تواريخ الأحداث: | Date Created: 19860201 Date Completed: 19860313 Latest Revision: 20190628 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/0003-9861(86)90610-7 |
| PMID: | 3511846 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0003-9861 |
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| DOI: | 10.1016/0003-9861(86)90610-7 |