دورية أكاديمية
Expression of bta-miR-222 and LHCGR in bovine cultured granulosa cells: Impact of follicle deviation and regulation by FSH/insulin in vitro.
| العنوان: | Expression of bta-miR-222 and LHCGR in bovine cultured granulosa cells: Impact of follicle deviation and regulation by FSH/insulin in vitro. |
|---|---|
| المؤلفون: | Santos PH; São Paulo State University (Unesp), Department of Pharmacology, Institute of Biosciences, Botucatu, SP, Brazil., Nunes SG; São Paulo State University (Unesp), Department of Pharmacology, Institute of Biosciences, Botucatu, SP, Brazil., Franchi FF; São Paulo State University (Unesp), Department of Pharmacology, Institute of Biosciences, Botucatu, SP, Brazil., Giroto AB; University of Western São Paulo (Unoeste), Presidente Prudente, SP, Brazil., Fontes PK; São Paulo State University (Unesp), Department of Pharmacology, Institute of Biosciences, Botucatu, SP, Brazil., Pinheiro VG; São Paulo State University (Unesp), Department of Pharmacology, Institute of Biosciences, Botucatu, SP, Brazil., Castilho ACS; University of Western São Paulo (Unoeste), Presidente Prudente, SP, Brazil. Electronic address: castilho.anthony@gmail.com. |
| المصدر: | Theriogenology [Theriogenology] 2022 Apr 01; Vol. 182, pp. 71-77. Date of Electronic Publication: 2022 Jan 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [New York, N.Y.?] : Elsevier Original Publication: Los Altos, Calif., Geron-X. |
| مواضيع طبية MeSH: | Follicle Stimulating Hormone*/metabolism , Follicle Stimulating Hormone*/pharmacology , MicroRNAs*/genetics , MicroRNAs*/metabolism, Animals ; Cattle ; Cells, Cultured ; Female ; Granulosa Cells/metabolism ; Insulin/metabolism ; Insulin/pharmacology ; Ovarian Follicle |
| مستخلص: | Final antral follicle development and future ovulation are mediated by gonadotropin-induced changes with spatio-temporally regulated expression of genes. Here, we aimed to quantify the relative mRNA abundance of bta-miR-222 and its predicted target, LHCGR, in granulosa cells (GCs) from follicles, after follicle deviation, as well as from GCs cultured in vitro with follicle stimulating hormone (FSH) and/or insulin. Thus, to study the impact of follicle deviation, Nelore heifers (n = 10; Bos taurus indicus) were hormonally synchronized and slaughtered 3 days after ovulation. Then, GCs from the dominant follicle (DF) and its respective subordinate follicle (SF) were recovered for RT-qPCR. For in vitro analysis, small follicles (2-5 mm) were dissected from bovine ovaries collected from a local abattoir. The GCs were isolated and cultured in serum-free medium, or treated with insulin (1 ng/mL or 10 ng/mL) alone or in combination with human recombinant FSH (1 ng/mL), for 6 days. Our findings showed that the relative mRNA abundance of LHCGR in GCs was higher in the DF compared to the SF (p = 0.01). Inversely, bta-miR-222 expression was lower in the DF compared to the SF (p = 0.01). Furthermore, GCs cultured with FSH and insulin together resulted in a higher abundance of LHCGR and a lower abundance of bta-miR-222 (p ≤ 0.05) when compared to GCs cultured with insulin alone. In conclusion, we found that the LHCGR upregulation in GCs from the DF is inversely related to bta-miR-222 expression. We also suggest the involvement of FSH in bta-miR-222 suppression in healthy bovine GCs. Competing Interests: Declaration of interest We declare that there is no conflict of present work. (Copyright © 2022 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antral follicle; Cattle; Follicle divergence; Gene expression; LHCGR; MicroRNA |
| المشرفين على المادة: | 0 (Insulin) 0 (MicroRNAs) 9002-68-0 (Follicle Stimulating Hormone) |
| تواريخ الأحداث: | Date Created: 20220208 Date Completed: 20220223 Latest Revision: 20220223 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.theriogenology.2022.01.034 |
| PMID: | 35131675 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3231 |
|---|---|
| DOI: | 10.1016/j.theriogenology.2022.01.034 |