دورية أكاديمية
Development of a new ultra-high-performance liquid chromatography-tandem mass spectrometry method for the determination of digoxin and digitoxin in plasma: Comparison with a clinical immunoassay.
| العنوان: | Development of a new ultra-high-performance liquid chromatography-tandem mass spectrometry method for the determination of digoxin and digitoxin in plasma: Comparison with a clinical immunoassay. |
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| المؤلفون: | Ballotari M; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy., Taus F; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy., Tolle G; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy., Danese E; Section of Clinical Biochemistry, University of Verona, Verona, Italy., Dorizzi RM; Clinical Pathology Unit, Hub Laboratory, Greater Area, Cesena, Italy., Tagliaro F; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.; World-Class Research Center 'Digital Biodesign and Personalized Healthcare', Sechenov First Moscow State Medical University, Moscow, Russia., Gottardo R; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy. |
| المصدر: | Electrophoresis [Electrophoresis] 2022 May; Vol. 43 (9-10), pp. 1019-1026. Date of Electronic Publication: 2022 Feb 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 8204476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2683 (Electronic) Linking ISSN: 01730835 NLM ISO Abbreviation: Electrophoresis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: [Weinheim, Germany] : Verlag Chemie, [1980- |
| مواضيع طبية MeSH: | Digitoxin*/chemistry , Digoxin*/chemistry, Chromatography, High Pressure Liquid/methods ; Chromatography, Liquid ; Immunoassay ; Tandem Mass Spectrometry/methods |
| مستخلص: | Cardiac glycosides digoxin and digitoxin are used in therapy for the treatment of congestive heart failure. Moreover, these compounds can be responsible for intoxication cases caused by fortuitous ingestion of leaves of Digitalis. Due to the narrow therapeutic range of these drugs, therapeutic drug monitoring is recommended in the clinical practice. In this context, immunoassays-based methods are generally employed but digoxin- and digitoxin-like compounds can interfere with the analysis. The aim of this study was to develop and validate an original UPLC-MS/MS method for the determination of digoxin and digitoxin in plasma. The method shows adequate sensitivity and selectivity with acceptable matrix effects and very good linearity, accuracy, precision, and recovery. A simple liquid-liquid extraction procedure was used for sample clean-up. The method was applied for the analysis of n = 220 plasma samples collected in two different clinical chemistry laboratories and previously tested by the same immunoassay. The statistical comparison showed a relevant negative bias of the UPLC-MS/MS method versus the immunoassay. These results are consistent with an immunoassay overestimation of digoxin plasmatic levels due to cross-reaction events with endogenous digoxin-like substances. (© 2022 The Authors. Electrophoresis published by Wiley-VCH GmbH.) |
| References: | J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jan 26;978-979:138-44. (PMID: 25544010) Toxicol Rev. 2006;25(4):273-81. (PMID: 17288498) Am J Cardiovasc Drugs. 2018 Dec;18(6):427-440. (PMID: 30066080) Sci Rep. 2020 Oct 1;10(1):16200. (PMID: 33004837) Clin Biochem. 2019 Jan;63:102-105. (PMID: 30316751) Biomed Pharmacother. 2020 Mar;123:109813. (PMID: 31924598) Exp Hematol Oncol. 2012 Apr 05;1(1):4. (PMID: 23210930) Eur J Clin Invest. 2001;31 Suppl 2:10-7. (PMID: 11525233) Am J Clin Pathol. 2003 Feb;119(2):298-303. (PMID: 12580002) Glob Cardiol Sci Pract. 2021 Apr 30;2021(1):e202102. (PMID: 34036088) Ther Drug Monit. 2008 Apr;30(2):212-7. (PMID: 18367983) J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jan 15;975:65-76. (PMID: 25438245) Eur J Heart Fail. 2016 Aug;18(8):1072-81. (PMID: 27492641) Drug Test Anal. 2015 Oct;7(10):937-46. (PMID: 25735870) J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 15;869(1-2):126-32. (PMID: 18515196) J Chromatogr A. 2010 Jul 2;1217(27):4535-43. (PMID: 20537342) Biol Pharm Bull. 2007 Sep;30(9):1653-6. (PMID: 17827715) J Chromatogr B Biomed Sci Appl. 1997 Apr 25;692(1):101-9. (PMID: 9187389) Case Rep Cardiol. 2019 Nov 29;2019:9707428. (PMID: 31871798) J Anal Toxicol. 2009 Sep;33(7):372-8. (PMID: 19796507) J Cardiovasc Electrophysiol. 2021 Mar;32(3):878-879. (PMID: 33522637) Biomed Chromatogr. 2008 Jul;22(7):712-8. (PMID: 18317988) Clin Biochem. 2010 Feb;43(3):307-13. (PMID: 19833118) Bioanalysis. 2018 Sep 1;10(17):1401-1411. (PMID: 30182747) In Vivo. 2020 Nov-Dec;34(6):3723-3730. (PMID: 33144490) Anal Chem. 2003 Jul 1;75(13):3019-30. (PMID: 12964746) Bioanalysis. 2009 Apr;1(1):97-113. (PMID: 21083191) |
| فهرسة مساهمة: | Keywords: Digitoxin; Digoxin; Immunoassay; LC-MS/MS |
| المشرفين على المادة: | 73K4184T59 (Digoxin) E90NZP2L9U (Digitoxin) |
| تواريخ الأحداث: | Date Created: 20220208 Date Completed: 20220517 Latest Revision: 20220819 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC9303718 |
| DOI: | 10.1002/elps.202100290 |
| PMID: | 35132652 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1522-2683 |
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| DOI: | 10.1002/elps.202100290 |