دورية أكاديمية
أعرض في EDS

The transcriptional elongation factor CTR9 demarcates PRC2-mediated H3K27me3 domains by altering PRC2 subtype equilibrium.

التفاصيل البيبلوغرافية
العنوان: The transcriptional elongation factor CTR9 demarcates PRC2-mediated H3K27me3 domains by altering PRC2 subtype equilibrium.
المؤلفون: Chan NT; McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA., Huang J; School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA., Ma G; McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA., Zeng H; McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA., Donahue K; McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA., Wang Y; McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA., Li L; School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA.; Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA., Xu W; McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA.; UW Carbone Cancer Center, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA.
المصدر: Nucleic acids research [Nucleic Acids Res] 2022 Feb 28; Vol. 50 (4), pp. 1969-1992.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Breast Neoplasms*/genetics , Histones*/genetics , Histones*/metabolism , Phosphoproteins*/genetics , Transcription Factors*/genetics , Transcription Factors*/metabolism, Cell Line, Tumor ; Chromatin ; Craniofacial Abnormalities ; Female ; Humans ; Polycomb Repressive Complex 2/metabolism
مستخلص: CTR9 is the scaffold subunit in polymerase-associated factor complex (PAFc), a multifunctional complex employed in multiple steps of RNA Polymerase II (RNAPII)-mediated transcription. CTR9/PAFc is well known as an evolutionarily conserved elongation factor that regulates gene activation via coupling with histone modifications enzymes. However, little is known about its function to restrain repressive histone markers. Using inducible and stable CTR9 knockdown breast cancer cell lines, we discovered that the H3K27me3 levels are strictly controlled by CTR9. Quantitative profiling of histone modifications revealed a striking increase of H3K27me3 levels upon loss of CTR9. Moreover, loss of CTR9 leads to genome-wide expansion of H3K27me3, as well as increased recruitment of PRC2 on chromatin, which can be reversed by CTR9 restoration. Further, CTR9 depletion triggers a PRC2 subtype switch from the less active PRC2.2, to the more active PRC2.1 with higher methyltransferase activity. As a consequence, CTR9 depletion generates vulnerability that renders breast cancer cells hypersensitive to PRC2 inhibitors. Our findings that CTR9 demarcates PRC2-mediated H3K27me3 levels and genomic distribution provide a unique mechanism that explains the transition from transcriptionally active chromatin states to repressive chromatin states and sheds light on the biological functions of CTR9 in development and cancer.
(© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.)
References: Nature. 2017 Nov 9;551(7679):204-209. (PMID: 29088706)
Cell Rep. 2017 Oct 17;21(3):628-640. (PMID: 29045832)
Cell Rep. 2016 Oct 4;17(2):583-595. (PMID: 27705803)
Bioinformatics. 2018 Aug 15;34(16):2867-2869. (PMID: 29608647)
Dev Biol. 2013 Nov 1;383(1):15-27. (PMID: 24036311)
Nat Genet. 2019 Jun;51(6):1060-1066. (PMID: 31152164)
PLoS One. 2013 Dec 19;8(12):e84147. (PMID: 24367637)
Mol Cell. 2011 May 6;42(3):330-41. (PMID: 21549310)
Breast Cancer Res Treat. 2013 Apr;138(3):741-52. (PMID: 23539298)
Mol Cell. 2015 Mar 5;57(5):769-783. (PMID: 25620564)
Nucleic Acids Res. 2016 Jul 8;44(W1):W160-5. (PMID: 27079975)
Cell. 2009 Dec 24;139(7):1290-302. (PMID: 20064375)
PLoS One. 2013 Dec 19;8(12):e83737. (PMID: 24367611)
Mol Cell. 2018 May 3;70(3):422-434.e6. (PMID: 29681499)
Genes Dev. 2019 Aug 1;33(15-16):903-935. (PMID: 31123062)
Nature. 2010 Sep 23;467(7314):430-5. (PMID: 20720539)
Exp Hematol. 2015 Aug;43(8):698-712. (PMID: 26027790)
Cell Rep. 2016 Jul 19;16(3):696-706. (PMID: 27396330)
Nucleic Acids Res. 2013 Jan 7;41(1):e28. (PMID: 23066101)
EMBO Rep. 2007 Sep;8(9):858-63. (PMID: 17721442)
Mol Cell. 2014 Aug 7;55(3):347-60. (PMID: 24999238)
Mol Cell. 2021 Feb 4;81(3):488-501.e9. (PMID: 33338397)
Blood. 2015 Jan 1;125(1):13-21. (PMID: 25320243)
Sci Rep. 2017 May 19;7(1):2193. (PMID: 28526819)
Science. 2015 Dec 11;350(6266):1383-6. (PMID: 26659056)
Elife. 2020 Mar 10;9:. (PMID: 32155117)
ACS Med Chem Lett. 2012 Oct 19;3(12):1091-6. (PMID: 24900432)
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4408-13. (PMID: 19246391)
Lancet Oncol. 2018 May;19(5):649-659. (PMID: 29650362)
Genes Dev. 2005 Jul 15;19(14):1668-73. (PMID: 16024656)
Genet Vaccines Ther. 2011 Apr 17;9(1):9. (PMID: 21496330)
Mol Cell. 2019 Apr 4;74(1):8-18. (PMID: 30951652)
Curr Opin Cell Biol. 2015 Dec;37:42-8. (PMID: 26497635)
Mol Cell. 2019 Nov 7;76(3):437-452.e6. (PMID: 31521505)
Theranostics. 2019 Jan 24;9(3):761-777. (PMID: 30809307)
Mol Cell. 2004 Jul 2;15(1):57-67. (PMID: 15225548)
ACS Chem Biol. 2013;8(6):1324-34. (PMID: 23614352)
Genes Dev. 2019 Oct 1;33(19-20):1428-1440. (PMID: 31488577)
Development. 2016 Aug 1;143(15):2716-23. (PMID: 27317809)
Biochim Biophys Acta. 2013 Jan;1829(1):116-26. (PMID: 22982193)
Genome Biol. 2008;9(9):R137. (PMID: 18798982)
Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286)
Bioinformatics. 2015 Jul 15;31(14):2382-3. (PMID: 25765347)
Mol Cell. 2018 Apr 19;70(2):371-379.e5. (PMID: 29606589)
G3 (Bethesda). 2016 Dec 7;6(12):3849-3857. (PMID: 27678520)
Elife. 2019 Jan 25;8:. (PMID: 30681409)
Mol Cell. 2018 May 3;70(3):408-421.e8. (PMID: 29628311)
Mol Cell Proteomics. 2016 Nov;15(11):3450-3460. (PMID: 27634302)
Biochim Biophys Acta. 2010 May-Jun;1799(5-6):379-88. (PMID: 20060942)
J Biol Chem. 2002 Feb 8;277(6):4324-33. (PMID: 11713257)
Nat Struct Mol Biol. 2021 Oct;28(10):778. (PMID: 34608338)
Nature. 2017 Sep 14;549(7671):287-291. (PMID: 28869966)
Drugs. 2020 Apr;80(5):513-521. (PMID: 32166598)
Nature. 2018 Aug;560(7720):607-612. (PMID: 30135578)
Nat Struct Mol Biol. 2012 Dec;19(12):1257-65. (PMID: 23104054)
Genes Dev. 2015 Oct 15;29(20):2153-67. (PMID: 26494790)
Mol Cell. 2016 Nov 17;64(4):645-658. (PMID: 27863225)
Cell Death Dis. 2019 Jun 26;10(7):502. (PMID: 31243265)
Cancer Res. 2018 Jan 15;78(2):410-421. (PMID: 29180470)
Nat Genet. 2018 Jul;50(7):1002-1010. (PMID: 29808031)
Science. 2018 Feb 23;359(6378):940-944. (PMID: 29348366)
PLoS One. 2012;7(5):e38278. (PMID: 22693611)
Mol Cell. 2018 Jun 21;70(6):1149-1162.e5. (PMID: 29932905)
Trends Biochem Sci. 2017 Jul;42(7):531-542. (PMID: 28483375)
Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. (PMID: 24550272)
Nat Med. 2016 Feb;22(2):128-34. (PMID: 26845405)
Nat Chem Biol. 2017 Apr;13(4):381-388. (PMID: 28135235)
Sci Rep. 2016 Dec 12;6:38970. (PMID: 27941919)
Nat Commun. 2014 Aug 07;5:4398. (PMID: 25099282)
Nucleic Acids Res. 2013 Dec;41(22):10619-29. (PMID: 24038468)
Trends Biochem Sci. 2017 Oct;42(10):788-798. (PMID: 28870425)
Biochim Biophys Acta. 2012 May;1824(5):759-68. (PMID: 22446411)
BMC Genomics. 2016 Nov 9;17(1):902. (PMID: 27829357)
Cell. 2015 Oct 8;163(2):506-19. (PMID: 26451490)
Trends Cell Biol. 2019 Aug;29(8):660-671. (PMID: 31178244)
Nat Commun. 2012 May 15;3:842. (PMID: 22588304)
EMBO J. 2004 Oct 13;23(20):4061-71. (PMID: 15385962)
Nucleus. 2014 May-Jun;5(3):224-36. (PMID: 24879308)
معلومات مُعتمدة: R01 CA268183 United States CA NCI NIH HHS; R01 CA236356 United States CA NCI NIH HHS; R01 CA213293 United States CA NCI NIH HHS; P30 CA014520 United States CA NCI NIH HHS; U01 CA231081 United States CA NCI NIH HHS; S10 RR029531 United States RR NCRR NIH HHS; P41 GM108538 United States GM NIGMS NIH HHS; R01 DK071801 United States DK NIDDK NIH HHS; T32 CA009135 United States CA NCI NIH HHS
سلسلة جزيئية: Dryad 10.5061/dryad.r7sqv9sd7
المشرفين على المادة: 0 (CTR9 protein, human)
0 (Chromatin)
0 (Histones)
0 (Phosphoproteins)
0 (Transcription Factors)
EC 2.1.1.43 (Polycomb Repressive Complex 2)
SCR Disease Name: Preauricular Fistulae, Congenital
تواريخ الأحداث: Date Created: 20220209 Date Completed: 20220415 Latest Revision: 20230126
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8887485
DOI: 10.1093/nar/gkac047
PMID: 35137163
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkac047