دورية أكاديمية
Binary Toxin Expression by Clostridioides difficile Is Associated With Worse Disease.
| العنوان: | Binary Toxin Expression by Clostridioides difficile Is Associated With Worse Disease. |
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| المؤلفون: | Young MK; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA., Leslie JL; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA., Madden GR; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA., Lyerly DM; TechLab Inc, Blacksburg, Virginia, USA., Carman RJ; TechLab Inc, Blacksburg, Virginia, USA., Lyerly MW; TechLab Inc, Blacksburg, Virginia, USA., Stewart DB; Department of Surgery, Southern Illinois University School of Medicine, Springfield, Illinois, USA., Abhyankar MM; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA., Petri WA Jr; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA.; Department of Microbiology, Immunology and Cancer Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.; Department of Pathology, University of Virginia School of Medicine, Charlottesville, Virginia, USA. |
| المصدر: | Open forum infectious diseases [Open Forum Infect Dis] 2022 Jan 10; Vol. 9 (3), pp. ofac001. Date of Electronic Publication: 2022 Jan 10 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by Oxford University Press on behalf of the Infectious Diseases Society of America Country of Publication: United States NLM ID: 101637045 Publication Model: eCollection Cited Medium: Print ISSN: 2328-8957 (Print) Linking ISSN: 23288957 NLM ISO Abbreviation: Open Forum Infect Dis Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Cary, NC : Published by Oxford University Press on behalf of the Infectious Diseases Society of America, [2014]- |
| مستخلص: | Background: The incidence of Clostridioides difficile infection (CDI) has increased over the past 2 decades and is considered an urgent threat by the Centers for Disease Control and Prevention. Hypervirulent strains such as ribotype 027, which possess genes for the additional toxin C. difficile binary toxin (CDT), are contributing to increased morbidity and mortality. Methods: We retrospectively tested stool from 215 CDI patients for CDT by enzyme-linked immunosorbent assay (ELISA). Stratifying patients by CDT status, we assessed if disease severity and clinical outcomes correlated with CDT positivity. Additionally, we completed quantitative PCR (PCR) DNA extracted from patient stool to detect cdtB gene. Lastly, we performed 16 S rRNA gene sequencing to examine if CDT-positive samples had an altered fecal microbiota. Results: We found that patients with CdtB, the pore-forming component of CDT, detected in their stool by ELISA, were more likely to have severe disease with higher 90-day mortality. CDT-positive patients also had higher C. difficile bacterial burden and white blood cell counts. There was no significant difference in gut microbiome diversity between CDT-positive and -negative patients. Conclusions: Patients with fecal samples that were positive for CDT had increased disease severity and worse clinical outcomes. Utilization of PCR and testing for C. difficile toxins A and B may not reveal the entire picture when diagnosing CDI; detection of CDT-expressing strains is valuable in identifying patients at risk of more severe disease. (© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America.) |
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| معلومات مُعتمدة: | R01 AI152477 United States AI NIAID NIH HHS; F32 DK124048 United States DK NIDDK NIH HHS; T32 AI007496 United States AI NIAID NIH HHS; UL1 TR003015 United States TR NCATS NIH HHS; R01 AI124214 United States AI NIAID NIH HHS; KL2 TR003016 United States TR NCATS NIH HHS; K23 AI163368 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: C. difficile; CDI; CDT; binary toxin; ribotype 027 |
| تواريخ الأحداث: | Date Created: 20220211 Latest Revision: 20240511 |
| رمز التحديث: | 20240511 |
| مُعرف محوري في PubMed: | PMC8825761 |
| DOI: | 10.1093/ofid/ofac001 |
| PMID: | 35146046 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2328-8957 |
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| DOI: | 10.1093/ofid/ofac001 |