دورية أكاديمية
Sugar nucleotide regeneration system for the synthesis of Bi- and triantennary N-glycans and exploring their activities against siglecs.
| العنوان: | Sugar nucleotide regeneration system for the synthesis of Bi- and triantennary N-glycans and exploring their activities against siglecs. |
|---|---|
| المؤلفون: | Anwar MT; Department of Chemistry, National Tsing-Hua University, Hsinchu, 30013, Taiwan., Kawade SK; Department of Chemistry, National Tsing-Hua University, Hsinchu, 30013, Taiwan., Huo YR; Department of Chemistry, National Tsing-Hua University, Hsinchu, 30013, Taiwan., Adak AK; Department of Chemistry, National Tsing-Hua University, Hsinchu, 30013, Taiwan., Sridharan D; Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan., Kuo YT; Department of Chemistry, National Tsing-Hua University, Hsinchu, 30013, Taiwan., Fan CY; Department of Chemistry, National Tsing-Hua University, Hsinchu, 30013, Taiwan., Wu HR; Instrumentation Center, National Tsing Hua University, Hsinchu, 30013, Taiwan., Lee YS; Department of Chemistry, National Tsing-Hua University, Hsinchu, 30013, Taiwan., Angata T; Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan. Electronic address: angata@gate.sinica.edu.tw., Lin CC; Department of Chemistry, National Tsing-Hua University, Hsinchu, 30013, Taiwan; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan. Electronic address: cclin66@mx.nthu.edu.tw. |
| المصدر: | European journal of medicinal chemistry [Eur J Med Chem] 2022 Mar 15; Vol. 232, pp. 114146. Date of Electronic Publication: 2022 Jan 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 0420510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1768-3254 (Electronic) Linking ISSN: 02235234 NLM ISO Abbreviation: Eur J Med Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Paris : Editions Scientifiques Elsevier Original Publication: Paris, S.E.C.T. [etc.] |
| مواضيع طبية MeSH: | Polysaccharides*/chemistry , Sialic Acid Binding Immunoglobulin-like Lectins*/metabolism, Nucleotides ; Sugars |
| مستخلص: | Enzymatic synthesis that is commenced by the sugar nucleotide regeneration system (SNRS) protocol can minimize 1) the consumption of exorbitant sugar nucleotides, 2) the amount of transferases required, and 3) byproduct feedback inhibition. In this study, LacNAc extensions/modifications of the N-linked mannose core were carried out efficiently with SNRS with high yields and purities on all branches in a uniform manner. In addition, we demonstrate that with SNRS, bacterial glycosyltransferases exhibit a wide acceptor tolerance for bi- and triantennary mannose core structures as substrates for target oligosaccharides. The synthesized small library of mannose core-based glycans and linear O-glycans were screened for their binding affinity against h-Siglecs 2, 4, 7, 9, 14, 15, and m-Siglec-15 to explore their structure-based binding preferences. Microarray data revealed that each Siglec showed few distinct yet overlapping specificities. An increase in branching from mono to di or tri antennary did not necessarily lead to increasing affinity. Glycans with the disialoside sequence α(2,3)α(2,8)/α(2,6)α(2,8) showed high specificity and affinity for Siglec-7, and sLex α(2,3) exhibited a strong affinity for Siglec-9. Explicit recognition of α(2,6)α(2,3)- linear and α(2,3)α(2,6)-branched glycans by Siglecs-2, 4, and 15, respectively, suggests that these structures can act as potential candidates for the further development of high-affinity ligands. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier Masson SAS. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Enzymatic synthesis; Glycan microarray; N-Glycan; Sialoside; Siglec; Sugar nucleotide regeneration |
| المشرفين على المادة: | 0 (Nucleotides) 0 (Polysaccharides) 0 (Sialic Acid Binding Immunoglobulin-like Lectins) 0 (Sugars) |
| تواريخ الأحداث: | Date Created: 20220212 Date Completed: 20220316 Latest Revision: 20220531 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.ejmech.2022.114146 |
| PMID: | 35149460 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1768-3254 |
|---|---|
| DOI: | 10.1016/j.ejmech.2022.114146 |