دورية أكاديمية
أعرض في EDS

Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos.

التفاصيل البيبلوغرافية
العنوان: Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos.
المؤلفون: El-Sokary MMM; Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt., El-Naby AAH; Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt., Hameed ARAE; Department of Animal Reproduction and A.I., Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt., Mahmoud KGM; Department of Animal Reproduction and A.I., Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt., Scholkamy TH; Department of Field Investigations, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt.
المصدر: Veterinary world [Vet World] 2021 Dec; Vol. 14 (12), pp. 3164-3169. Date of Electronic Publication: 2021 Dec 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Veterinary World Country of Publication: India NLM ID: 101504872 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0972-8988 (Print) Linking ISSN: 09728988 NLM ISO Abbreviation: Vet World Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: Dist. Morbi (Gujarat), India : Veterinary World
Original Publication: Rajkot (Gujarat), India : Veterinary World, [2008]-
مستخلص: Background and Aim: Despite many trials, buffalo embryos have poor cryosurvivability because of their high lipid content. L-carnitine was found to be a lipid-reducing agent when added to oocyte and embryo culture media. The study aimed to determine the most effective concentration of L-carnitine to improve the oocyte developmental competence and cryotolerance of buffalo embryos.
Materials and Methods: In vitro maturation and embryo culture media were supplemented with four concentrations of L-carnitine: 0 (control), 0.25, 0.5, and 1 mM. Good-quality embryos on 7 days were vitrified using mixtures of dimethyl sulfoxide and ethylene glycol at two concentrations (3.5 and 7 M).
Results: The result showed that the cleavage and morula rates were significantly (p<0.05) higher in the 0.5 mM group. Blastocyst rates were significantly (p<0.05) higher at both 0.5 and 1 mM. The rates of viable embryos directly after thawing were significantly (p<0.05) increased in the 0.5 mM group. No significant difference was found in embryos cultured for 24 h after warming among all the groups.
Conclusion: The addition of L-carnitine at a concentration of 0.5 mM to the culture media improves the oocyte developmental competence and cryotolerance of buffalo embryos directly after warming but not after 24 h of culture. Nevertheless, further studies must identify how L-carnitine exerts its beneficial micromechanisms.
(Copyright: © El-Sokary, et al.)
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فهرسة مساهمة: Keywords: L-carnitine; antioxidant; buffalo embryos; cryotolerance
تواريخ الأحداث: Date Created: 20220214 Latest Revision: 20220501
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8829399
DOI: 10.14202/vetworld.2021.3164-3169
PMID: 35153408
قاعدة البيانات: MEDLINE
الوصف
تدمد:0972-8988
DOI:10.14202/vetworld.2021.3164-3169