دورية أكاديمية

Bovine coronavirus in the lower respiratory tract of cattle with respiratory disease.

التفاصيل البيبلوغرافية
العنوان: Bovine coronavirus in the lower respiratory tract of cattle with respiratory disease.
المؤلفون: Rahe MC; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA., Magstadt DR; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA., Groeltz-Thrush J; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA., Gauger PC; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA., Zhang J; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA., Schwartz KJ; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA., Siepker CL; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
المصدر: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2022 May; Vol. 34 (3), pp. 482-488. Date of Electronic Publication: 2022 Feb 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Sage Country of Publication: United States NLM ID: 9011490 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1943-4936 (Electronic) Linking ISSN: 10406387 NLM ISO Abbreviation: J Vet Diagn Invest Subsets: MEDLINE
أسماء مطبوعة: Publication: 2011-: Thousand Oaks, CA : Sage
Original Publication: 1989-2004 : Columbia, MO : American Association of Veterinary Laboratory Diagnosticians
مواضيع طبية MeSH: Cattle Diseases* , Coronavirus Infections*/veterinary , Coronavirus, Bovine* , Respiratory Tract Infections*/veterinary, Animals ; Cattle ; Respiratory System/pathology
مستخلص: Bovine coronavirus (BCoV) is a known cause of enteric disease in cattle; however, its role in bovine respiratory disease (BRD) is poorly understood, with a dearth of evidence of the detection of the virus in respiratory tract lesions. We coupled histologic evaluation of tracheal and lower airway tissues from 104 calves with BRD in which BCoV was detected in the lungs via PCR followed by direct detection of BCoV by immunohistochemistry and an RNA in situ hybridization assay (ISH; RNAscope technology). RNAscope ISH detected BCoV in respiratory epithelium in more cases than did IHC. Using both methods of direct detection, tracheal epithelial attenuation and identification of the virus within lesions were observed commonly. Our results confirm a role of BCoV in respiratory tract infection and pathology, and show that the virus likely plays a role in the development of BRD.
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فهرسة مساهمة: Keywords: RNAscope; bovine coronavirus; histopathology; immunohistochemistry; respiratory disease
تواريخ الأحداث: Date Created: 20220216 Date Completed: 20220503 Latest Revision: 20230217
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9254051
DOI: 10.1177/10406387221078583
PMID: 35168437
قاعدة البيانات: MEDLINE
الوصف
تدمد:1943-4936
DOI:10.1177/10406387221078583