دورية أكاديمية
BrpNAC895 and BrpABI449 coregulate the transcription of the afflux-type Cd transporter BrpHMA2 in Brassica parachinensis.
العنوان: | BrpNAC895 and BrpABI449 coregulate the transcription of the afflux-type Cd transporter BrpHMA2 in Brassica parachinensis. |
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المؤلفون: | Liu S; Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, China.; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.; Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi 710003, China., Li L; Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China.; Life Sciences College, Zhaoqing University, Zhaoqing, 526061, China., Deng Y; Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, China., Bai Y; Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, China., Sun C; College of Horticulture, Nanjing Agricultural University, No.1 Weigang, 8210095 Nanjing, China., Huang S; Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, China., Zhou J; Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, China., Shi L; Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, China., Yang X; Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, China., Li L; Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China., Chen X; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, USA., Tang Y; Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, China. |
المصدر: | Horticulture research [Hortic Res] 2022 Feb 19. Date of Electronic Publication: 2022 Feb 19. |
Publication Model: | Ahead of Print |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101655540 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2662-6810 (Print) Linking ISSN: 20527276 NLM ISO Abbreviation: Hortic Res |
أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: London : Nature Publishing Group in partnership with Nanjing Agricultural University |
مستخلص: | Brassica parachinensis is a popular leafy vegetable. It is able to accumulate high concentration of Cd, however, the molecular mechanism of Cd accumulation is unknown. This study investigated the function and regulatory mechanism of the Cd-responsive metal ion transporter gene BrpHMA2. BrpHMA2 was induced by Cd stress and specifically expressed in vascular tissues, and the protein was localized in the plasma membrane. Heterologous expression of BrpHMA2 enhanced Cd accumulation and Cd sensitivity in transgenic Arabidopsis and yeast. After Cd stress, the transcriptional factors BrpNAC895 and BrpABI449, which may recognize the ABREs in the BrpHMA2 promoter, were also differentially expressed. The transcriptional regulation of BrpHMA2 was further investigated using ChIP-qPCR, EMSA and LUC reporter activity analysis employing the transient expression system of Brassica parachinensis protoplasts and tobacco leaves and the E. coli expression system. By binding to the promoter, BrpNAC895 induced the transcription of BrpHMA2. BrpABI449 might bind to the BrpHMA2 promoter or interact with BrpNAC895 to interfere with the action of BrpNAC895. The findings suggest that BrpHMA2 is a membrane-based afflux-type Cd transporter involved in the Cd2+ uptake and long-distance transport in plants. BrpNAC895 and BrpABI449, which function as the transcription activator and repressor respectively, coregulate BrpHMA2 expression. (© The Author(s) 2022. Published by Oxford University Press. All rights reserved.) |
References: | Plant Sci. 2011 Feb;180(2):169-81. (PMID: 21421358) Bioessays. 2018 Aug;40(8):e1800018. (PMID: 29938806) Front Plant Sci. 2016 Jul 19;7:1075. (PMID: 27486475) Biochemistry. 2007 Jul 3;46(26):7754-64. (PMID: 17550234) Plant Physiol. 2013 May;162(1):424-39. (PMID: 23530184) Plant Physiol. 2017 Mar;173(3):1565-1573. (PMID: 28073984) Plant Cell Rep. 2018 Sep;37(9):1343-1352. (PMID: 29936635) Plant Cell. 2015 Mar;27(3):787-805. (PMID: 25794933) Plant Physiol Biochem. 2020 Feb;147:280-288. (PMID: 31891862) New Phytol. 2013 May;198(3):721-731. (PMID: 23421478) Plant J. 2010 Feb;61(4):672-85. (PMID: 19947981) Plant J. 2013 Jul;75(1):26-39. (PMID: 23578292) Plant Biotechnol J. 2013 May;11(4):420-31. (PMID: 23294838) Plant Cell. 2013 Aug;25(8):3039-51. (PMID: 23995086) Food Chem Toxicol. 2011 Sep;49(9):2260-7. (PMID: 21693161) Cell Mol Life Sci. 2012 Oct;69(19):3187-206. (PMID: 22903262) Plant Cell. 2004 May;16(5):1327-39. (PMID: 15100400) New Phytol. 2009;181(1):71-78. (PMID: 19076718) Front Plant Sci. 2016 Dec 16;7:1884. (PMID: 28018412) Plant Cell. 2014 Mar;26(3):1294-307. (PMID: 24596241) J Plant Res. 2011 Jul;124(4):509-25. (PMID: 21416314) Plant Cell. 2018 Sep;30(9):2197-2213. (PMID: 30099383) Nat Plants. 2021 Feb;7(2):159-171. (PMID: 33594264) Trends Plant Sci. 2005 Oct;10(10):491-502. (PMID: 16154798) J Biol Chem. 2006 Nov 10;281(45):33881-91. (PMID: 16973620) Plant Cell. 2019 Mar;31(3):663-686. (PMID: 30538157) Plant Methods. 2013 Feb 05;9(1):4. (PMID: 23379342) Sci Total Environ. 2021 Apr 20;766:142663. (PMID: 33153750) Plant Methods. 2005 Dec 18;1:13. (PMID: 16359558) Plant Physiol. 2019 May;180(1):529-542. (PMID: 30782964) Mol Plant. 2012 Jan;5(1):27-42. (PMID: 21873619) J Bacteriol. 1983 Jan;153(1):163-8. (PMID: 6336730) Mol Plant. 2019 Apr 1;12(4):489-505. (PMID: 30639313) Nat Biotechnol. 2020 Jan;38(1):84-89. (PMID: 31844292) Bot Stud. 2019 Aug 9;60(1):16. (PMID: 31399930) Plant Cell. 2021 Jul 2;33(5):1594-1614. (PMID: 33793897) Front Genet. 2020 May 08;11:477. (PMID: 32457808) Plant Cell Physiol. 2012 Jan;53(1):213-24. (PMID: 22123790) J Mol Biol. 2021 Aug 6;433(16):166991. (PMID: 33865868) Plant J. 2021 Feb;105(3):600-618. (PMID: 33119146) Plant Physiol. 2013 Jun;162(2):927-39. (PMID: 23575418) Plant Cell. 2013 Nov;25(11):4708-24. (PMID: 24285786) Plant Physiol. 2004 Nov;136(3):3712-23. (PMID: 15475410) |
فهرسة مساهمة: | Keywords: Brassica parachinensis; ABI449; AREB (ABA responsive element binding proteins); Cadmium (Cd); Heavy metal ATPase 2 (HMA2); NAC895; Transcription factors (TFs); Transporter |
تواريخ الأحداث: | Date Created: 20220220 Latest Revision: 20240220 |
رمز التحديث: | 20240220 |
مُعرف محوري في PubMed: | PMC9045254 |
DOI: | 10.1093/hr/uhac044 |
PMID: | 35184182 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2662-6810 |
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DOI: | 10.1093/hr/uhac044 |