دورية أكاديمية
HPLC-DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae).
العنوان: | HPLC-DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae). |
---|---|
المؤلفون: | de Freitas MA; Federal University of Pernambuco (UFPE), Recife, PE Brazil., da Cruz RP; Regional University of Cariri (URCA), Crato, CE Brazil., Dos Santos ATL; Regional University of Cariri (URCA), Crato, CE Brazil., Almeida-Bezerra JW; Regional University of Cariri (URCA), Crato, CE Brazil., Machado AJT; Regional University of Cariri (URCA), Crato, CE Brazil., Dos Santos JFS; Regional University of Cariri (URCA), Crato, CE Brazil., Rocha JE; Regional University of Cariri (URCA), Crato, CE Brazil., Boligon AA; Federal University of Santa Maria (UFSM), Santa Maria, RS Brazil., Bezerra CF; Federal University of Pernambuco (UFPE), Recife, PE Brazil., de Freitas TS; Regional University of Cariri (URCA), Crato, CE Brazil., do Nascimento Silva MK; Regional University of Cariri (URCA), Crato, CE Brazil., Mendonça ACAM; Regional University of Cariri (URCA), Crato, CE Brazil., da Costa JGM; Regional University of Cariri (URCA), Crato, CE Brazil., Coutinho HDM; Regional University of Cariri (URCA), Crato, CE Brazil., da Cunha FAB; Regional University of Cariri (URCA), Crato, CE Brazil., Filho JR; Oswaldo Cruz Foundation, Salvador, BA Brazil., Morais-Braga MFB; Regional University of Cariri (URCA), Crato, CE Brazil. |
المصدر: | 3 Biotech [3 Biotech] 2022 Mar; Vol. 12 (3), pp. 61. Date of Electronic Publication: 2022 Feb 07. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 101565857 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2190-572X (Print) Linking ISSN: 21905738 NLM ISO Abbreviation: 3 Biotech Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Berlin : Springer |
مستخلص: | Spondias mombin is used in the folk medicine for the treatment of diarrhea and dysentery, indicating that extracts obtained from this species may present pharmacological activities against pathogenic microorganisms. The purpose of this work was to investigate the chemical composition and evaluate the antimicrobial activity of extracts obtained from the leaves (aqueous) and bark (hydroethanolic) of S. mombin both as single treatments and in combination with conventional drugs. Following a qualitative chemical prospection, the extracts were analyzed by HPLC-DAD. The antimicrobial activities were evaluated by microdilution. The combined activity of drugs and extracts was verified by adding a subinhibitory concentration of the extract in the presence of variable drug concentrations. The Minimum Fungicidal Concentration (MFC) was determined by a subculture of the microdilution test, while the effect of the in vitro treatments on morphological transition was analyzed by subculture in moist chambers. While the qualitative analysis detected the presence of phenols and flavonoids, the HPLC analysis identified quercetin, caffeic acid, and catechin as major components in the leaf extract, whereas kaempferol and quercetin were found as major compounds in the bark extract. The extracts showed effective antibacterial activities only against the Gram-negative strains. With regard to the combined activity, the leaf extract potentiated the action of gentamicin and imipenem (against Staphylococcus aureus ), while the bark extract potentiated the effect of norfloxacin (against S. aureus ), imipenem (against Escherichia coli ), and norfloxacin (against Pseudomonas aeruginosa ). A more significant antifungal (fungistatic) effect was achieved with the bark extract (even though at high concentrations), which further enhanced the activity of fluconazole. The extracts also inhibited the emission of filaments by Candida albicans and Candida tropicalis . Together, these findings suggest that that the extract constituents may act by favoring the permeability of microbial cells to conventional drugs, as well as by affecting virulence mechanisms in Candida strains . (© King Abdulaziz City for Science and Technology 2022.) |
References: | J Ethnopharmacol. 2007 Apr 4;110(3):391-400. (PMID: 17317057) Antibiotics (Basel). 2020 Jan 27;9(2):. (PMID: 32012710) BMC Complement Altern Med. 2018 Oct 19;18(1):284. (PMID: 30340567) Molecules. 2021 May 03;26(9):. (PMID: 34063685) Biofouling. 2019 Mar;35(3):320-328. (PMID: 31066306) J Fungi (Basel). 2020 Jan 17;6(1):. (PMID: 31963458) Comp Immunol Microbiol Infect Dis. 2019 Aug;65:58-64. (PMID: 31300127) Molecules. 2015 Sep 29;20(10):17903-12. (PMID: 26426003) Afr J Med Med Sci. 2012 Dec;41 Suppl:99-103. (PMID: 23678643) PLoS One. 2018 Mar 9;13(3):e0193300. (PMID: 29522559) J Ethnopharmacol. 2012 Feb 15;139(3):838-57. (PMID: 22222280) Nat Rev Microbiol. 2019 Mar;17(3):141-155. (PMID: 30683887) Antibiotics (Basel). 2019 Nov 28;8(4):. (PMID: 31795107) Pathogens. 2021 May 01;10(5):. (PMID: 34062722) Front Microbiol. 2020 Jun 25;11:1437. (PMID: 32670252) Future Microbiol. 2014;9(4):445-56. (PMID: 24810344) Pharmaceuticals (Basel). 2020 Dec 30;14(1):. (PMID: 33396973) Infect Drug Resist. 2018 Oct 10;11:1645-1658. (PMID: 30349322) Clin Microbiol Infect. 2019 Jul;25(7):792-798. (PMID: 30965100) J Ethnopharmacol. 2020 Mar 1;249:112411. (PMID: 31751651) Antibiotics (Basel). 2020 Aug 31;9(9):. (PMID: 32878266) 3 Biotech. 2021 Jul;11(7):317. (PMID: 34123696) Infect Dis (Lond). 2018 Mar;50(3):175-192. (PMID: 29105519) Biomed Pharmacother. 2020 Nov;131:110716. (PMID: 32920516) J Taibah Univ Med Sci. 2018 Apr 12;13(3):262-271. (PMID: 31435333) Lancet Infect Dis. 2019 Jan;19(1):56-66. (PMID: 30409683) Diagn Microbiol Infect Dis. 1999 Feb;33(2):75-80. (PMID: 10091029) Fungal Biol. 2020 Feb;124(2):83-90. (PMID: 32008756) Microb Pathog. 2016 Jun;95:200-207. (PMID: 27085299) Antibiotics (Basel). 2020 Jan 14;9(1):. (PMID: 31947642) Lancet Infect Dis. 2018 Nov;18(11):1229-1240. (PMID: 30266330) Nature. 2016 Mar 3;531(7592):64-9. (PMID: 26901871) PLoS One. 2020 Nov 6;15(11):e0241304. (PMID: 33156838) Int J Med Microbiol. 2018 Aug;308(6):607-624. (PMID: 29217333) Pathogens. 2020 Sep 16;9(9):. (PMID: 32947768) Antibiotics (Basel). 2020 May 12;9(5):. (PMID: 32408576) J Med Chem. 1996 Aug 2;39(16):3107-13. (PMID: 8759631) An Acad Bras Cienc. 2017;89(3 Suppl):2423-2432. (PMID: 28746624) J Mycol Med. 2017 Mar;27(1):33-45. (PMID: 27743771) Comp Immunol Microbiol Infect Dis. 2019 Jun;64:14-19. (PMID: 31174689) Biomed Res Int. 2015;2015:807670. (PMID: 25821822) Chemotherapy. 2008;54(4):328-30. (PMID: 18698137) |
فهرسة مساهمة: | Keywords: Antibacterial; Antifungal; Antimicrobial resistance; Phytochemicals |
تواريخ الأحداث: | Date Created: 20220221 Latest Revision: 20230302 |
رمز التحديث: | 20230302 |
مُعرف محوري في PubMed: | PMC8818589 |
DOI: | 10.1007/s13205-022-03126-1 |
PMID: | 35186658 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2190-572X |
---|---|
DOI: | 10.1007/s13205-022-03126-1 |