دورية أكاديمية
أعرض في EDS

The transient Spt4-Spt5 complex as an upstream regulator of non-coding RNAs during development.

التفاصيل البيبلوغرافية
العنوان: The transient Spt4-Spt5 complex as an upstream regulator of non-coding RNAs during development.
المؤلفون: Owsian D; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland., Gruchota J; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland., Arnaiz O; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France., Nowak JK; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
المصدر: Nucleic acids research [Nucleic Acids Res] 2022 Mar 21; Vol. 50 (5), pp. 2603-2620.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Genome, Protozoan* , Paramecium tetraurelia*/cytology , Paramecium tetraurelia*/genetics , Paramecium tetraurelia*/growth & development, Meiosis ; RNA, Untranslated/genetics ; Transcription Factors/metabolism ; Transcription, Genetic
مستخلص: The Spt4-Spt5 complex is conserved and essential RNA polymerase elongation factor. To investigate the role of the Spt4-Spt5 complex in non-coding transcription during development, we used the unicellular model Paramecium tetraurelia. In this organism harboring both germline and somatic nuclei, massive transcription of the entire germline genome takes place during meiosis. This phenomenon starts a series of events mediated by different classes of non-coding RNAs that control developmentally programmed DNA elimination. We focused our study on Spt4, a small zinc-finger protein encoded in P. tetraurelia by two genes expressed constitutively and two genes expressed during meiosis. SPT4 genes are not essential in vegetative growth, but they are indispensable for sexual reproduction, even though genes from both expression families show functional redundancy. Silencing of the SPT4 genes resulted in the absence of double-stranded ncRNAs and reduced levels of scnRNAs - 25 nt-long sRNAs produced from these double-stranded precursors in the germline nucleus. Moreover, we observed that the presence of a germline-specific Spt4-Spt5m complex is necessary for transfer of the scnRNA-binding PIWI protein between the germline and somatic nucleus. Our study establishes that Spt4, together with Spt5m, is essential for expression of the germline genome and necessary for developmental genome rearrangements.
(© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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المشرفين على المادة: 0 (RNA, Untranslated)
0 (Transcription Factors)
تواريخ الأحداث: Date Created: 20220221 Date Completed: 20220415 Latest Revision: 20220531
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8934623
DOI: 10.1093/nar/gkac106
PMID: 35188560
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkac106