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The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.

التفاصيل البيبلوغرافية
العنوان: The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.
المؤلفون: Mahoney BJ; Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, California, USA., Takayesu A; Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA., Zhou A; Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA., Cascio D; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, California, USA., Clubb RT; Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California, USA.
المصدر: Proteins [Proteins] 2022 Jul; Vol. 90 (7), pp. 1457-1467. Date of Electronic Publication: 2022 Feb 28.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8700181 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0134 (Electronic) Linking ISSN: 08873585 NLM ISO Abbreviation: Proteins Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Wiley-Liss
Original Publication: New York : Alan R. Liss, c1986-
مواضيع طبية MeSH: Cellulosomes*/chemistry , Clostridium thermocellum*/metabolism, Bacterial Proteins/chemistry ; Biomass ; Cellulose/metabolism ; Proline/metabolism ; Sigma Factor/chemistry
مستخلص: Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass. An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes. Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors. Here we show that the RsgI9 anti-σ factor interacts with cellulose via a C-terminal bi-domain unit. A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose. Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation. In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface. The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
(© 2022 Wiley Periodicals LLC.)
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معلومات مُعتمدة: P30 GM124169 United States GM NIGMS NIH HHS; S10 OD025073 United States OD NIH HHS; R01-AI052217 United States NH NIH HHS; R01 AI052217 United States AI NIAID NIH HHS; S10OD025073 United States NH NIH HHS; S10 OD016336 United States OD NIH HHS; P30 GM124165 United States GM NIGMS NIH HHS; S10OD016336 United States NH NIH HHS; S10OD018483 NIGMS ALS-ENABLE; S10 OD018483 United States OD NIH HHS
فهرسة مساهمة: Keywords: anti-sigma factor; biofuels; biomass; cellulosomes; crystallography; small-angle X-ray scattering
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Sigma Factor)
9004-34-6 (Cellulose)
9DLQ4CIU6V (Proline)
تواريخ الأحداث: Date Created: 20220223 Date Completed: 20220608 Latest Revision: 20230102
رمز التحديث: 20230102
مُعرف محوري في PubMed: PMC9177573
DOI: 10.1002/prot.26326
PMID: 35194841
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-0134
DOI:10.1002/prot.26326