دورية أكاديمية
Prevalence and Characterization of PVL-Positive Staphylococcus aureus Isolated from Raw Cow's Milk.
العنوان: | Prevalence and Characterization of PVL-Positive Staphylococcus aureus Isolated from Raw Cow's Milk. |
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المؤلفون: | Sadat A; Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Shata RR; Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Farag AMM; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Ramadan H; Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Alkhedaide A; Clinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21995, Saudi Arabia., Soliman MM; Clinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21995, Saudi Arabia., Elbadawy M; Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Elqaliobiya, Egypt., Abugomaa A; Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Awad A; Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. |
المصدر: | Toxins [Toxins (Basel)] 2022 Jan 25; Vol. 14 (2). Date of Electronic Publication: 2022 Jan 25. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel : MDPI |
مواضيع طبية MeSH: | Bacterial Toxins/*genetics , Exotoxins/*genetics , Leukocidins/*genetics , Methicillin-Resistant Staphylococcus aureus/*isolation & purification , Milk/*microbiology, Animals ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Drug Resistance, Bacterial/genetics ; Food Microbiology ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Methicillin-Resistant Staphylococcus aureus/genetics ; Virulence/genetics |
مستخلص: | The present study aimed to investigate the prevalence, antibiotic susceptibility profiles, and some toxin genes of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus ( S. aureus ) in unpasteurized raw cow's milk collected from retail outlets located at Mansoura, Dakahliya governorate, Egypt. In that context, a total of 700 raw cow's milk samples were investigated for the presence of S. aureus , which was identified in 41.1% (288/700) of the samples. Among the S. aureus isolates, 113 PVL-positive S. aureus were identified and subjected for further analysis. The PVL-positive S. aureus were investigated for the existence of toxin-related genes, including hemolysin ( hla ), toxic shock syndrome toxin-1 ( tst ), and enterotoxins ( sea , seb , sec , see , seg , sei , and selj ). Genotypic resistance of PVL-positive strains was performed for the detection of blaZ and mecA genes. Among the PVL-positive S. aureus , sea , seb , and sec were detected in 44.2, 6.2%, and 0.9%, respectively, while the hla and tst genes were identified in 54.9% and 0.9%, respectively. The blaZ and mecA genes were successfully identified in 84.9 (96/113) and 32.7% (37/113) of the total evaluated S. aureus isolates, respectively. PVL-positive S. aureus displayed a high level of resistance to penicillin, ampicillin, and trimethoprim-sulfamethoxazole. Multidrug resistance (resistant to ≥3 antimicrobial classes) was displayed by all methicillin-resistant S. aureus (MRSA) and 38.2% of methicillin-sensitive S. aureus (MSSA) isolates. The obtained findings are raising the alarm of virulent PVL-positive MRSA clones in retail milk in Egypt, suggesting the requirement for limiting the use of β-lactam drugs in food-producing animals and the importance of implementing strong hygiene procedures in dairy farms and processing plants. |
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فهرسة مساهمة: | Keywords: MRSA; Staphylococcus aureus; antimicrobial resistance; enterotoxins; pvl gene |
المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Bacterial Proteins) 0 (Bacterial Toxins) 0 (Exotoxins) 0 (Leukocidins) 0 (Panton-Valentine leukocidin) |
تواريخ الأحداث: | Date Created: 20220224 Date Completed: 20220323 Latest Revision: 20220323 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8876356 |
DOI: | 10.3390/toxins14020097 |
PMID: | 35202125 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2072-6651 |
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DOI: | 10.3390/toxins14020097 |