دورية أكاديمية
أعرض في EDS

Prevalence and Characterization of PVL-Positive Staphylococcus aureus Isolated from Raw Cow's Milk.

التفاصيل البيبلوغرافية
العنوان: Prevalence and Characterization of PVL-Positive Staphylococcus aureus Isolated from Raw Cow's Milk.
المؤلفون: Sadat A; Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Shata RR; Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Farag AMM; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Ramadan H; Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Alkhedaide A; Clinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21995, Saudi Arabia., Soliman MM; Clinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21995, Saudi Arabia., Elbadawy M; Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Elqaliobiya, Egypt., Abugomaa A; Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Awad A; Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
المصدر: Toxins [Toxins (Basel)] 2022 Jan 25; Vol. 14 (2). Date of Electronic Publication: 2022 Jan 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Bacterial Toxins/*genetics , Exotoxins/*genetics , Leukocidins/*genetics , Methicillin-Resistant Staphylococcus aureus/*isolation & purification , Milk/*microbiology, Animals ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Drug Resistance, Bacterial/genetics ; Food Microbiology ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Methicillin-Resistant Staphylococcus aureus/genetics ; Virulence/genetics
مستخلص: The present study aimed to investigate the prevalence, antibiotic susceptibility profiles, and some toxin genes of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus ( S. aureus ) in unpasteurized raw cow's milk collected from retail outlets located at Mansoura, Dakahliya governorate, Egypt. In that context, a total of 700 raw cow's milk samples were investigated for the presence of S. aureus , which was identified in 41.1% (288/700) of the samples. Among the S. aureus isolates, 113 PVL-positive S. aureus were identified and subjected for further analysis. The PVL-positive S. aureus were investigated for the existence of toxin-related genes, including hemolysin ( hla ), toxic shock syndrome toxin-1 ( tst ), and enterotoxins ( sea , seb , sec , see , seg , sei , and selj ). Genotypic resistance of PVL-positive strains was performed for the detection of blaZ and mecA genes. Among the PVL-positive S. aureus , sea , seb , and sec were detected in 44.2, 6.2%, and 0.9%, respectively, while the hla and tst genes were identified in 54.9% and 0.9%, respectively. The blaZ and mecA genes were successfully identified in 84.9 (96/113) and 32.7% (37/113) of the total evaluated S. aureus isolates, respectively. PVL-positive S. aureus displayed a high level of resistance to penicillin, ampicillin, and trimethoprim-sulfamethoxazole. Multidrug resistance (resistant to ≥3 antimicrobial classes) was displayed by all methicillin-resistant S. aureus (MRSA) and 38.2% of methicillin-sensitive S. aureus (MSSA) isolates. The obtained findings are raising the alarm of virulent PVL-positive MRSA clones in retail milk in Egypt, suggesting the requirement for limiting the use of β-lactam drugs in food-producing animals and the importance of implementing strong hygiene procedures in dairy farms and processing plants.
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فهرسة مساهمة: Keywords: MRSA; Staphylococcus aureus; antimicrobial resistance; enterotoxins; pvl gene
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Bacterial Proteins)
0 (Bacterial Toxins)
0 (Exotoxins)
0 (Leukocidins)
0 (Panton-Valentine leukocidin)
تواريخ الأحداث: Date Created: 20220224 Date Completed: 20220323 Latest Revision: 20220323
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8876356
DOI: 10.3390/toxins14020097
PMID: 35202125
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6651
DOI:10.3390/toxins14020097