دورية أكاديمية
أعرض في EDS

Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need.

التفاصيل البيبلوغرافية
العنوان: Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need.
المؤلفون: Zohny MH; Department of Biochemistry, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 11152, Egypt. Electronic address: munazohny@gmail.com., Cavalu S; Faculty of Medicine and Pharmacy, University of Oradea, P-ta 1 Decembrie 10, 410087 Oradea, Romania. Electronic address: simona.cavalu@gmail.com., Youssef ME; Department of Pharmacology, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 11152, Egypt. Electronic address: mahmoodelsaid@hotmail.com., Kaddah MMY; Pharmaceutical and Fermentation Industries Development Center, City of Scientific Research and Technological Applications, New Borg El-Arab 21934, Alexandria, Egypt. Electronic address: mmkaddah1978@gmail.com., Mourad AAE; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Port Said University, Port Said 42511, Egypt. Electronic address: ahmed.mourad@yahoo.com., Gaafar AGA; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Port Said University, Port Said 42511, Egypt. Electronic address: agafer777@yahoo.com., El-Ahwany E; Department of Immunology, Theodor Bilharz Research Institute, Giza, Egypt. Electronic address: ahwany@aucegypt.edu., Amin NA; Department of Haematology, Theodor Bilharz Research Institute, Giza 12411, Egypt. Electronic address: esmaealn78@gmail.com., Arakeep HM; Department of Anatomy and Embryology, Faculty of Medicine, Tanta University, Tanta, Egypt. Electronic address: dr.hebaarakeep83@gmail.com., Shata A; Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Mansoura, Egypt; Department of Clinical Pharmacy, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, Egypt. Electronic address: ahmedmhes@mans.edu.eg., Saleh S; Department of Clinical Physiology, Faculty of Medicine, Menoufia University, Menoufia, Egypt. Electronic address: safaa_kotb48@yahoo.com., Hafez MM; Department of Biochemistry, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt. Electronic address: mohkhalifa2000@yahoo.com., Elazab ST; Department of Pharmacology, Faculty of Veterinary medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: Sara.taha@ymail.com., Abdelhady R; Pharmacology and Toxicology Department, Faculty of Pharmacy, Fayoum University, Fayoum, Egypt. Electronic address: ram14@fayoum.edu.eg., El Shahat RM; Department of Pharmacology, Faculty of Medicine, Al-Azhar University, Cairo 11884, Egypt. Electronic address: rehab@azhar.edu.eg., Yahya G; Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Al Sharqia, Egypt. Electronic address: galalmetwally2020@gmail.com., Saber S; Department of Pharmacology, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 11152, Egypt. Electronic address: sameh.mohamed@deltauniv.edu.eg.
المصدر: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2022 Apr; Vol. 148, pp. 112723. Date of Electronic Publication: 2022 Feb 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 8213295 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1950-6007 (Electronic) Linking ISSN: 07533322 NLM ISO Abbreviation: Biomed Pharmacother Subsets: MEDLINE
أسماء مطبوعة: Publication: Paris : Editions Scientifiques Elsevier
Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982-
مواضيع طبية MeSH: NF-kappa B*/metabolism , Pulmonary Fibrosis*/chemically induced , Pulmonary Fibrosis*/drug therapy , Pulmonary Fibrosis*/metabolism, Animals ; Bleomycin/toxicity ; Inflammasomes/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Rats ; Rosaniline Dyes
مستخلص: Pulmonary fibrosis (PF) is a life-threatening disorder with a very poor prognosis. Because of the complexity of PF pathological mechanisms, filling such an unmet medical need is challenging. A number of pulmonary diseases have been linked to the activation of NF-κB and the NLRP3 inflammasome. Coomassie brilliant blue G-250 (CBBG) is proved to be a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome. This is the first report to investigate the effect of CBBG on the bleomycin-induced lung fibrosis in rats. Our findings revealed that CBBG resulted in a significant improvement in histological features and oxidative status biomarkers of bleomycin-exposed lung tissue. Additionally, CBBG repressed collagen deposition as indicated after the analysis of hydroxyproline, TGF-β, PDGF-BB, TIMP-1, MMP-9, Col1a1, SMA and ICAM-1. It also exhibited anti-inflammatory potential as revealed by the determination of TNF-α, IL-1β, IL-18, MCP-1 in the lung tissue. In the bronchoalveolar lavage, the total protein and the LDH activity were substantially reduced. The lung protective effects of CBBG might be attributed on the one hand to the inhibition of NLRP3 inflammasome and on the other hand to the inactivation of NF-κB. Decreased levels of phospho-p65 and its DNA-binding activity as well as the analysis of TLR4 confirmed NF-κB inactivation. Caspase-1 activity is suppressed as a consequence of inhibiting NLRP3 inflammasome assembly. To conclude, CBBG may act as a primary or adjuvant therapy for the management of PF and therefore it may pose an opportunity for a novel approach to an unmet medical need.
(Copyright © 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
فهرسة مساهمة: Keywords: Bleomycin; Coomassie brilliant blue G-250; Lung fibrosis; NF-κB; NLRP3
المشرفين على المادة: 0 (Inflammasomes)
0 (NF-kappa B)
0 (NLR Family, Pyrin Domain-Containing 3 Protein)
0 (Nlrp3 protein, rat)
0 (Rosaniline Dyes)
11056-06-7 (Bleomycin)
M1ZRX790SI (coomassie Brilliant Blue)
تواريخ الأحداث: Date Created: 20220224 Date Completed: 20220407 Latest Revision: 20220407
رمز التحديث: 20231215
DOI: 10.1016/j.biopha.2022.112723
PMID: 35202914
قاعدة البيانات: MEDLINE
الوصف
تدمد:1950-6007
DOI:10.1016/j.biopha.2022.112723