دورية أكاديمية

Spatiotemporal expression pattern of miR-205, miR-26a-5p, miR-17-5p, let-7b-5p, and their target genes during different stages of corpus luteum in Egyptian buffaloes.

التفاصيل البيبلوغرافية
العنوان: Spatiotemporal expression pattern of miR-205, miR-26a-5p, miR-17-5p, let-7b-5p, and their target genes during different stages of corpus luteum in Egyptian buffaloes.
المؤلفون: Ibrahim S; Department of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt. sally_rashad2004@yahoo.com., Taqi MO; Central Laboratory for Agricultural Climate, Agricultural Research Centre, Ministry of Agriculture and Land Reclamation, Dokki, Giza, 12311, Egypt., Sosa ASA; Department of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt., El-Naby AAH; Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Banha, Egypt., Mahmoud KGM; Department of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt., Darwish HRH; Cell Biology Department, Institute of Biotechnology Research, National Research Centre, Dokki, 12622, Giza, Egypt., Abd El Hameed AR; Department of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt., Nawito MF; Department of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.
المصدر: Journal, genetic engineering & biotechnology [J Genet Eng Biotechnol] 2022 Feb 25; Vol. 20 (1), pp. 37. Date of Electronic Publication: 2022 Feb 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101317150 Publication Model: Electronic Cited Medium: Internet ISSN: 2090-5920 (Electronic) Linking ISSN: 1687157X NLM ISO Abbreviation: J Genet Eng Biotechnol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2024- : [Amsterdam, Netherlands] : Elsevier B.V.
Original Publication: [Cairo] : Academy of Scientific Research and Technology, Information and Scientific Services Sector, National Information and Documentation Center
مستخلص: Background: No doubt that the corpus luteum (CL) plays a vital role in the regulation of female cyclicity in mammals. The scenarios among microRNAs (miRNAs) and their target genes and steroid hormones {estradiol (E2) and progesterone (P4)} are required for better understanding the molecular regulation of CL during its formation, maturation, and regression. We aimed to (I) study the changes in the relative abundance of miR-205, miR-26a-5p, miR-17-5p, and let-7b-5p and their target genes: LHCGR, CASP3, PCNA, AMH, and PLA2G3, during different stages of corpus luteum in Egyptian buffaloes, and (II) and to address different scenarios between steroid concentrations in the serum and the expression pattern of selected miRNAs and their targets.
Methods: The paired ovaries and blood samples were collected from apparently healthy 50 buffalo cows at a private abattoir. The ovaries bearing CL were macroscopically divided according to their morphological structure and color into hemorrhagic (CLH), developing (CLD), mature (CLM), regressed (CLR), and albicans (CLA). Small pieces from different stages of CL (CLH, CLD, CLM, CLR, and CLA) were cut and immediately kept at - 80 °C for total RNA isolation and qRT-PCR. The serum was separated for steroid level estimation.
Results: The LHCGR was expressed during different stages of CL, and the peak of expression was at the mid-luteal stage. The CASP3 revealed a stage-specific response at different stages of CL. The PCNA has an essential role in cellular proliferation in buffaloes CL. Both expression patterns of PLA2G3 and AMH were found over the various developmental and regression stages. It was noticed that miR-205 is conserved to target LHCGR and CASP3 transcripts. Moreover, CASP3 and AMH were targeted via miR-26a-5p. Additionally, the CASP3 and PLA2G3 were targeted via let-7b-5p. The P4 level reached its peak during CLM. There were positive and negative strong correlations between miRNAs (miR-26a-5p and miR-205), target genes (LHCGR and CASP3) during different stages of CL, and steroid hormones in the serum.
Conclusions: Taken together, the orchestrated pattern among miRNAs, target genes, and steroid hormones is essential for maintaining the proper development and function of CL in buffalo cows.
(© 2022. The Author(s).)
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معلومات مُعتمدة: ID:3/I-3-B-6116-1 This work was financially supported by a grant (ID:3/I-3-B-6116-1) from International Foundation for Science (Sweden) and Organisation of Islamic Cooperation's Standing Committee on Scientific and Technological Cooperation (COMSTECH)
فهرسة مساهمة: Keywords: Buffaloes; Corpus luteum; MicroRNAs; Serum steroids; Target genes
تواريخ الأحداث: Date Created: 20220225 Latest Revision: 20221005
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8881532
DOI: 10.1186/s43141-022-00320-9
PMID: 35212793
قاعدة البيانات: MEDLINE
الوصف
تدمد:2090-5920
DOI:10.1186/s43141-022-00320-9