دورية أكاديمية
Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens.
| العنوان: | Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens. |
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| المؤلفون: | Ali AR; Department of Medical Microbiology and Immunology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt., Anani HAA; Department of Medical Microbiology and Immunology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt., Selim FM; Department of Medical Microbiology and Immunology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt. |
| المصدر: | Iranian journal of microbiology [Iran J Microbiol] 2021 Dec; Vol. 13 (6), pp. 848-861. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Tehran University of Medical Sciences Country of Publication: Iran NLM ID: 101518404 Publication Model: Print Cited Medium: Print ISSN: 2008-3289 (Print) Linking ISSN: 20083289 NLM ISO Abbreviation: Iran J Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Tehran : Tehran University of Medical Sciences |
| مستخلص: | Background and Objectives: Silver nanoparticles (AgNPs) have been found to have multiple uses as antibacterial, antifungal and anti-biofilm agents because of their biological activities and safety. The present study was aimed to analyze the antimicrobial and anti-biofilm activities as well as the cytotoxic effect of AgNPs against different human pathogens. Materials and Methods: AgNPs were synthesized using cell free supernatants of Escherichia coli (ATCC 25922), Enterococcus faecalis (ATCC 19433), Pseudomonas aeruginosa (ATCC 27856), Enterobacter cloacae (ATCC 13047) and Penicillium oxalicum strain, then were analyzed using UV/Vis Spectral Analysis, Transmission electron microscopy (TEM). Scanning Electron Microscope (SEM) and Energy Dispersive-X-ray Spectroscopy (EDX) analysis. Antimicrobial activities of biosynthesized AgNPs were assessed with selected antimicrobial agents against multidrug resistant bacteria and candida. Anti-biofilm and cytotoxicity assays of these biosynthesized AgNPs were also done. Results: The synthesis of AgNPs were confirmed through observed color change and monitoring UV-Vis spectrum which showed homogeneous (little agglomeration) distribution of silver nanoparticles. TEM and SEM have shown that the particle size ranged from 13 to 34 (nm) with spherical shape and a high signal with EDX analysis. Antibacterial and antifungal efficacy of antibiotics and fluconazole were increased in combination with biosynthesized AgNPs against resistant bacteria and candida. Significant reduction in biofilm formation was found better with Penicillium oxalicum AgNPs against biofilm forming bacteria. Conclusion: Penicillium oxalicum has the best effect towards synthesizing AgNPs, for antimicrobial activities against resistant bacteria and candida, in addition to anti-biofilm activities against biofilm forming Staphylococcus aureus and E. coli and the safest cytotoxicity effect on (MRC-5) cell line. (Copyright © 2021 The Authors. Published by Tehran University of Medical Sciences.) |
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| فهرسة مساهمة: | Keywords: Biofilm; Cytotoxicity; Penicillium oxalicum; Sliver nanoparticles |
| تواريخ الأحداث: | Date Created: 20220228 Latest Revision: 20220502 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8816695 |
| DOI: | 10.18502/ijm.v13i6.8090 |
| PMID: | 35222864 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2008-3289 |
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| DOI: | 10.18502/ijm.v13i6.8090 |